Calcium Signaling Pathways Involved In Long-Term Potentiation At Excitatory Synapses On Parvalbumin Positive Fast-Spiking GABAergic Neurons In The Mouse Visual Cortex

Document Type : Original Article


1 Brain Science Institute RIKEN, Hirosawa, Japan.

2 Physiology Department, Hamadan University of Medical Sciences, Hamadan, Iran

3 Brain Science Institute RIKEN, Hirosawa, Japan


Objective: Recently, we reported the presence of metabotropic glutamate receptor type 5 (mGluR-5) dependent long-term potentiation (LTP) at excitatory synapses on fast-spiking GABAergic (FS-GABA) cells in the visual cortex. In this study, we report a Ca2+ signaling pathway involved in this LTP type.
Materials and Methods: Brain slices from GAD67-GFP knock-in mice were used. Using whole-cell patch-clamp recording followed by immunohistochemical staining on parvalbumin- positive (PV+) FS-GABA cells, we studied the Ca2+ signaling pathway involved in excitatory LTP of certain visual cortical interneuron subtypes.
Results: U-73122- a phospolipas c (PLC) inhibitor (10 μM), inositol triphosphate (IP3) inhibitors such as 2-APB (3 μM) and heparin (10 IU/ml), and CPA- the internally stored Ca2+ release inhibitor- (5 μM) blocked the mGluR5 signaling pathway to induce LTP at excitatory synapses on PV+ fast-spiking cells in the visual cortex. However, application of the vehicles alone had no effect.
Conclusion: Our results indicate that mGluR-5 at FS-GABA neurons activate PLC and IP3 production. This leads to Ca2+ release, promotes LTP induction, and its maintenance is supported by internal Ca2+ stores. Considering the key role of PV+ FS inhibitory neurons in the visual cortex circuits, we suggest that the metabotropic glutamate receptor-dependent LTP of excitatory synapses to FS cells plays a crucial role in the visual cortex plasticity.