FISHERIES SCIENCES DEPARTMENT, FACULTY OF NATURAL RESOURCES, UNIVERSITY OF KURDISTAN, SANANDAJ, IRAN
Abstract
Objective: Immunohistochemical localization and immunoreactivity of insulin-like growth factor-I (IGF-I) were investigated during the ovarian developmental stages in Persian sturgeon. In addition, the effects of growth hormone and thyroxine were investigated on IGF-I immunoreactivity in vitro. Materials and Methods: Ovarian samples were taken from two brood stocks (caught from the sea and from a river) during their reproductive migration and at three different developmental stages based on their polarization index (PI). The effects of two hormones on IGF-I immunoreactivity were studied at two ovarian developmental stages (PI > 0.1 and PI < 0.07). In both experiments the immunoperoxidase method was performed in order to detect immunohistochemical localization and immunoreactivity of IGF-I. Results: Immunohistochemical localizations of IGFI were detected in the follicular layers. There was no significant immunoreactivity difference between the two brood stocks, however in the river brood stock IGF-I immunoreactivity was more intense in the ovaries with PI < 0.07 than of PI > 0.1 (p < 0.05). Growth hormone (10 ng/ml) increased IGF-I immunoreactivity in ovarian samples from the river brood stock when their PI was less than 0.07, however thyroxine had not such effect (p < 0.05). Conclusion: Our results showed that IGF-I is present in the ovaries of Persian sturgeon and its reactivity is different among their gonadal development stages. This may support a role for IGF-I during reproductive physiology in female brood stocks of the Persian sturgeon. Moreover, growth hormone is a potential hormone to increase IGF-I immunoreactivity in the ovaries of this species.
Kamangar, B. B., Mojazi Amiri, B., Javad Rasaee, M., Abtahi, B., & Bahmani, M. (2010). Immunolocalization Of Insulin-Like Growth Factor-I And Its Immunoreactivity During Ovary Developmental Stages Of Persian Sturgeon Acipenser Persicus. Cell Journal (Yakhteh), 11(4), 432-441.
MLA
Barzan Bahrami Kamangar; Bagher Mojazi Amiri; Mohammad Javad Rasaee; Behrouz Abtahi; Mahmoud Bahmani. "Immunolocalization Of Insulin-Like Growth Factor-I And Its Immunoreactivity During Ovary Developmental Stages Of Persian Sturgeon Acipenser Persicus". Cell Journal (Yakhteh), 11, 4, 2010, 432-441.
HARVARD
Kamangar, B. B., Mojazi Amiri, B., Javad Rasaee, M., Abtahi, B., Bahmani, M. (2010). 'Immunolocalization Of Insulin-Like Growth Factor-I And Its Immunoreactivity During Ovary Developmental Stages Of Persian Sturgeon Acipenser Persicus', Cell Journal (Yakhteh), 11(4), pp. 432-441.
VANCOUVER
Kamangar, B. B., Mojazi Amiri, B., Javad Rasaee, M., Abtahi, B., Bahmani, M. Immunolocalization Of Insulin-Like Growth Factor-I And Its Immunoreactivity During Ovary Developmental Stages Of Persian Sturgeon Acipenser Persicus. Cell Journal (Yakhteh), 2010; 11(4): 432-441.
', './data/cellj/coversheet/cover_en.jpg'))