Document Type : Original Article
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Cardiovascular Engineering Lab, Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran
National CeNational Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iranll Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
Iranian Blood Transfusion Organization Research Center, Tehran, Iran
Objective: Mesenchymal stem cells (MSCs) can be expanded and differentiated into many mature cell types including smooth muscle cells (SMCs). In addition to growth factor, cyclic stretch contributes to differentiation of stem cells. Mechanical stimuli are critical to morphological changes, development, regeneration, differentiation and pathology of
mesenchymal tissues. The aim of this study is to investigate effects of cyclic stretch with differing amplitudes on morphology and differentiation of mesenchymal stem cells.
Materials and Methods: Mesenchymal stem cells are extracted from human bone marrow. Cells are cultured on silicone membrane and exposed to cyclic stretch by a custom made device. Cellular images are captured before and after tests. Effects of 5% and 15% uniaxial strain with 1Hz frequency and 1-8 hour durations on morphology of human mesenchymal stem cells are investigated. It is assumed that environmental factors such as mechanical loading regulate MSCs differentiation to SMCs. Fractal analysis is used to quantify alterations in cellular morphology. An image processing method with a designed code is used for evaluation of fractal dimension parameter.
Results: Results demonstrate statistically significant change in cell morphology due to mechanical stretch. By elevation of strain amplitude and number of load cycles, fractal dimensions of cell images decrease. Such decrease is equivalent to alignment of cells by mechanical stimulus. Cells are differentiated to SMCs purely by cyclic stretch. The initiation and rate of differentiation depend on mechanical conditions.
Conclusion: To produce functional SMCs for engineered tissues, MSCs can be exposed to uniaxial cyclic stretch. The functionality of differentiated SMCs depends on loading conditions.