Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Comparative Study of The Effects of Confounding Factors on Improving Rat Pancreatic Islet Isolation Yield and Quality49149925333610.22074/cellj.2022.8123ENMaedeh MoazenchiDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranAdvanced Therapy Medicinal Product Technology Development Center (ATMP-TDC), Cell Science Research Center, Royan Institute
for Stem Cell Biology and Technology, ACECR, Tehran, IranAnavasadat Sadr Hashemi NejadDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranAdvanced Therapy Medicinal Product Technology Development Center (ATMP-TDC), Cell Science Research Center, Royan Institute
for Stem Cell Biology and Technology, ACECR, Tehran, IranMahmoud IzadiDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranAdvanced Therapy Medicinal Product Technology Development Center (ATMP-TDC), Cell Science Research Center, Royan Institute
for Stem Cell Biology and Technology, ACECR, Tehran, IranMaedeh KhalajDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranZakieh SamsonchiDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranPouya Tavakol RadDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranPayam AminiReproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranYaser TahamtaniDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and
Technology, ACECR, Tehran, IranReproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranEnsiyeh Hajizadeh-SaffarAdvanced Therapy Medicinal Product Technology Development Center (ATMP-TDC), Cell Science Research Center, Royan Institute
for Stem Cell Biology and Technology, ACECR, Tehran, IranReproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR,
Tehran, Iran0000-0003-4933-5250Journal Article20210630<strong>Objective:</strong> Isolated pancreatic islets are valuable resources for a wide range of research, including cell replacement<br />studies and cell-based platforms for diabetes drug discovery and disease modeling. Islet isolation is a complex and stepwise procedure aiming to obtain pure, viable, and functional islets for in vitro and in vivo studies. It should be noted<br />that differences in rodent strains, gender, weight, and density gradients may affect the isolated islet’s properties. We evaluated the variables affecting the rat islet isolation procedure to reach the maximum islet yield and functionality, which would be critical for further studies on islet regenerative biology.<br /><strong>Materials and Methods: </strong>The present experimental study compared the yield and purity of isolated islets from nondiabetic rats of two different strains. Next, islet particle number (IPN) and islet equivalent (IEQ) were compared between males and females, and the weight range that yields the highest number of islets was investigated. Moreover, the influence of three different density gradients, namely Histopaque, Pancoll, and Lymphodex, on final isolated islets purity and yield were assessed. Finally, the viability and functionality of isolated islets were measured.<br /><strong>Results: </strong>The IEQ, IPN, and purity of isolated islets in 15 Lister hooded rats (LHRs) were significantly (P≤0.05) higher than<br />those of the other strains. Male LHRs resulted in significantly higher IEQ compared to females (P≤0.05). Moreover, IPN and IEQ did not significantly vary among different weight groups. Also, the utilization of Histopaque and Pancoll leads to higher yield and purity. In vivo assessments of the isolated islets presented significantly reduced blood glucose percentage in the transplanted group on days 2-5 following transplantation.<br /><strong>Conclusion:</strong> Based on these results, an optimal protocol for isolating high-quality rat islets with a constant yield, purity,<br />and function has been established as an essential platform for developing diabetes research.https://www.celljournal.org/article_253336_eb04db112ef5bd495e95575b1b3dd93e.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Microarray Profile of Circular RNAs Identifies hsa_circ_0001583 as A New Circular RNA Biomarker for Breast Cancer: A Retrospective Study50050525335910.22074/cellj.2022.8200ENMin ZhengDepartment of Oncology, Fujian Key Laboratory of Precision Medicine for Cancer, The First Affiliated Hospital of Fujian Medical
University, Fujian, Fuzhou, P. R. ChinaWei-Hua CaiDepartment of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Hospital of Fujian Medical University, Fujian,
Fuzhou, P. R. ChinaMou-Feng WangDepartment of Oncology, Fujian Key Laboratory of Precision Medicine for Cancer, The First Affiliated Hospital of Fujian Medical
University, Fujian, Fuzhou, P. R. ChinaYu-Jie DengDepartment of Oncology, Fujian Key Laboratory of Precision Medicine for Cancer, The First Affiliated Hospital of Fujian Medical
University, Fujian, Fuzhou, P. R. ChinaLing-ling HuangDepartment of Oncology, Fujian Key Laboratory of Precision Medicine for Cancer, The First Affiliated Hospital of Fujian Medical
University, Fujian, Fuzhou, P. R. ChinaYong-Jin CaoDepartment of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Hospital of Fujian Medical University, Fujian,
Fuzhou, P. R. ChinaJournal Article20210822<strong>Objective:</strong> Breast cancer (BC) is the most common cancer, which is currently the leading cause of cancer death. Circular RNAs (circRNAs) play important roles in cancer, however, circRNAs serving as vital index in BC for guiding treatment have not yet been identified. The aim of our study is to explore a novel kind of potential biomarker for BC.<br /><strong>Materials and Methods:</strong> In this retrospective study, the samples used for assays were two groups of breast tumor tissue obtained from four BC patients, including four pairs of tumor tissues and adjacent nontumor samples. The circRNA expression profiles were detected via microarray and validated by real-time quantitative polymerase chain reaction (PCR).<br /><strong>Results:</strong> The differentially expressed circRNAs in tested samples were screened and analyzed by using human circRNA<br />microarray. After analysis, considering a fold gene expression change of ≥2.0 and P<0.05, results suggested that 256 circRNAs were significantly up-regulated and 277 circRNAs were significantly down-regulated. Besides, the results of the real-time quantitative PCR assay showed that the expression of hsa_circ_0001583 was significantly up-regulated in BC groups (P<0.05) by real-time quantitative PCR. Therefore, we thought hsa_circ_0001583 might serve as a novel kind of biomarker for BC.<br /><strong>Conclusion: </strong>Hsa_circ_0001583 showed significant up-regulation in BC patients with paired adjacent tissues. Many cancer immune pathways were related to has_circ_0001583, including autoimmune thyroid disease, chemokine and T-cell receptor signaling pathways.https://www.celljournal.org/article_253359_0467248c7f6d57e98efa50dc6fd9b334.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds50651425334010.22074/cellj.2022.8005ENShima RahmatiCancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences,
Sanandaj, IranAli JaliliCancer and Immunology Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences,
Sanandaj, IranMehdi BanitalebiDehkordiDepartment of Molecular Medicine, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, IranMichelle PrzedborskiDepartment of Applied Mathematics, University of Waterloo, Waterloo, ON, CanadaJournal Article20210303<strong>Objective:</strong> Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin<br />regeneration in small wounds.<br /><strong>Materials and Methods:</strong> In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimized<br />foreskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility and<br />recellularization of both scaffolds by hucMSC were subsequently determined.<br /><strong>Results: </strong>We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength.<br /><strong>Conclusion:</strong> We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and the<br />mechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small woundshttps://www.celljournal.org/article_253340_56753af0c2d573773de649ea58be0aa5.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Modified Bismuth Nanoparticles: A New Targeted Nanoprobe for Computed Tomography Imaging of Cancer51552125335610.22074/cellj.2022.8348ENMilad MohammadiMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranSara KhademiDepartment of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, IranYazdan ChoghazrdiDepartment of Medical Physics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, IranRasoul IrajiradDepartment of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, IranMohammad KeshtkarMedical Physics and Radiology Department, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, IranAlireza MontazerabadiMedical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, IranJournal Article20211114Objective: Recently, development of multifunctional contrast agent for effective targeted molecular computed<br />tomography (CT) imaging of cancer cells stays a major problem. In this study, we explain the ability of Triptorelin<br />peptide-targeted multifunctional bismuth nanoparticles (Bi2S3@ BSA-Triptorelin NPs) for molecular CT imaging.<br />Materials and Methods: In this experimental study, the formed nanocomplex of Bi2S3@ BSA-Triptorelin NPs<br />was characterized using different methods. The MTT cytotoxicity test was performed to determine the appropriate<br />concentration of nanoparticles in the MCF-7 cells. The X-ray attenuation intensity and Contrast to Noise Ratio (CNR) of<br />targeted and non-targeted nanoparticles were measured at the concentrations of 25, 50, and 75 μg/ml and X-ray tube<br />voltages of 90, 120 and 140 kVp.<br />Results: We showed that the formed Bi2S3@ BSA-Triptorelin NPs with a Bi core size of approximately ~8.6 nm are nontoxic<br />in a given concentration (0-200 μg/ml). At 90, 120, and 140 tube potentials (kVp), the X-ray attenuation of targeted cells were<br />1.35, 1.36, and 1.33-times, respectively, more than non-targeted MCF-7cells at the concentration of 75 μg/ml. The CNR<br />values at 90, 120, and 140 kVp tube potentials were 171.5, 153.8 and 146.3 c/ϭ, respectively.<br />Conclusion: These findings propose that the diagnostic nanocomplex of Bi2S3@ BSA-Triptorelin NPs can be applied<br />as a good contrast medium for molecular CT techniques.https://www.celljournal.org/article_253356_351d3723fcaac951ed5dc3bd8ae78bca.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Ionizing Radiation Promotes Epithelial-Mesenchymal Transition Phenotype and Stem Cell Marker in The Lung adenocarcinoma: In Vitro and Bioinformatic Studiesc52253025335710.22074/cellj.2022.8403ENMehdi RaeiHealth Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, IranMahdi BagheriStudent Research Committee, Baqiyatallah University of Medical Sciences, Tehran, IranSafieh AghaabdollahianDepartment of Nanobiotechnology, New Technologies Research Group, Pasteur Institute of Iran, Tehran, IranMasoud GhorbaniApplied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IranAfshin SadeghiStudent Research Committee, Baqiyatallah University of Medical Sciences, Tehran, IranJournal Article20211231<strong>Objective</strong>: Ionizing radiation (IR) is one of the major therapeutic approaches in the non-small cell lung cancer (NSCLC);<br />however, it can paradoxically result in cancer progression likely through promoting epithelial-mesenchymal transition<br />(EMT) and the cancer stem cell phenotype. Therefore, we aimed to determine whether IR promote EMT/CSC and to<br />investigate the clinical relevance of EMT/CSC hallmark genes.<br /><strong>Materials and Methods</strong>: In this experimental and bioinformatic study, A549 cell line was irradiated with a high dosage<br />(6 Gy) or a fractionated regimen (2 Gy/day for 15 fractions). The EMT-related features, including cellular morphology,<br />migratory and invasive capacities were evaluated using scratch assay and transwell migration/invasion assays. The<br />mRNA levels of EMT-related genes (CDH1, CDH2, SNAI1 and TWIST1), stemness-related markers (CD44, PROM1,<br />and ALDH1A1) and the CDH2/CDH1 ratio were evaluated via real-time polymerase chain reaction (PCR). The clinical<br />significance of these genes was assessed in the lung adenocarcinoma (LUAD) samples using online databases.<br /><strong>Results</strong>: Irradiation resulted in a dramatic elongation of cell shape and enhanced invasion and migration capabilities. These EMT-like alterations were accompanied with enhanced levels of SNAI1, CDH2, TWIST1, CD44, PROM1, and ALDH1A1 as well as an enhanced CDH2/CDH1 ratio. TCGA analysis revealed that, TWIST1, CDH1, PROM1 and CDH2 were upregulated; whereas, CD44, SNAI1 and ALDH1A1 were downregulated. Additionally, correlations between SNAI1-TWIST1, CDH2- TWIST1, CDH2-SNAI1, and ALDH1A1-PROM1 was positive. Kaplan-Meier survival analysis identified lower expression of CDH1, PROM1 and ALDH1A1 and increased expression of CDH2, SNAI1, and TWIST1 as well as CDH2/CDH1 ratio predict overall survival. Additionally, downregulation of ALDH1A1 and upregulation of CDH2, SNAI1 and TWIST1 could predict a shorter first progression.<br /><strong>Conclusion</strong>: Altogether, these findings demonstrated that IR promotes EMT phenotype and stem cell markers in A549<br />cell line and these genes could function as diagnostic or prognostic indicators in LUAD samples.https://www.celljournal.org/article_253357_8284f91a242c25d18203c5debf44e745.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901The Global mRNA Expression Profiles of Inhibiting PHGDH Induced Cisplatin Resistance in Gastric Cancer53153925337110.22074/cellj.2022.8046ENKailing PanCentral Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, Zhejiang Province, ChinaXiaoya ZhaoCentral Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, Zhejiang Province, ChinaWenxia XuCentral Laboratory, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, Jinhua, Zhejiang
Province, ChinaJournal Article20210415<strong>Objective</strong>: Drug resistance is the main hindrance to improve the prognosis of patients with gastric cancer. Amino<br />acid metabolic reprograming is essential to satisfy the different requirements of cancer cells during drug resistance,<br />of which serine deprivation could promote resistance to cisplatin in gastric cancer. As the key enzyme in the de novo<br />biosynthesis of serine, phosphoglycerate dehydrogenase (PHGDH) inhibition could also induce cisplatin resistance in<br />gastric cancer. This study aims to reveal the potential mechanisms of drug resistance induced by PHGDH inhibition via<br />exploring the global mRNA expression profiles.<br /><strong>Materials and Methods</strong>: In this experimental study, the viability and the apoptotic rate of gastric cancer cells<br />were evaluated by using Cell Counting Kit-8 (CCK-8) analysis and flow cytometric determination, respectively. The<br />identification of differentially expressed genes (DEGs) was tested by mRNA-sequencing (mRNA-Seq) analysis. The<br />confirmation of sequencing results was verified using real-time quantitative reverse transcription polymerase chain<br />reaction (RT-qPCR).<br /><strong>Results:</strong> The inhibition of PHGDH significantly increased the viability and decreased the apoptotic rate induced by cisplatin<br />in gastric cancer cells. mRNA-Seq analysis revealed that the combined treatment of NCT503 reduced the number of DEGs<br />induced by cisplatin. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment<br />Analysis (GSEA) showed that unfolded protein response, ECM receptor interaction and cell cycle signaling pathways were<br />modulated by NCT503 treatment. Hub genes were identified by using protein-protein interaction network modeling, of which E1A binding protein p300 (EP300) and heat shock protein family A (Hsp70) member 8 (HSPA8) act as the vital genes in cisplatin resistance induced by the inhibition of PHGDH.<br /><strong>Conclusion:</strong> These findings suggested that the inhibition of PHGDH promoted cisplatin resistance in gastric cancer<br />through various intercellular mechanisms. And appropriate serine supplementation or the modulation of EP300 and<br />HSPA8 may be of great help in overcoming cisplatin resistance in gastric cancer.https://www.celljournal.org/article_253371_6e6ef4da32108b67d1e25608f5b5782f.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Altered Expression of GDF9 and BMP15 Genes in Granulosa Cells of Diminished Ovarian Reserve Patients: A Case-Control Study54054525333210.22074/cellj.2022.8077ENMaryam OmrizadehDepartment of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranPegah MokhtariDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran,IranPoopak Eftekhari-YazdiDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR,
Tehran, IranZahra ChekiniDepartment of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive
Biomedicine, ACECR, Tehran, IranDepartment of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, IranAnahita Mohseni MeybodiDepartment of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran,
IranDepartment of Pathology and Laboratory Medicine, Western University, London, Ontario, CanadaMolecular Genetics Laboratory, Molecular Diagnostics Division, London Health Sciences Centre, London, Ontario, CanadaJournal Article20210523<strong>Objective:</strong> Diminished ovarian reserve (DOR) is a challenging issue encountered during assisted reproductive technology. Growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) belong to the transforming growth factor-beta (TGF-β) superfamily which are essential for folliculogenesis. We aimed to the evaluation<br />of the GDF9 and BMP15 expression in the granulosa cells (GCs) of DOR patients.<br /><strong>Materials and Methods:</strong> This case-control study included 14 women with DOR and 12 controls, who were between 28-40 years of age undergoing controlled ovarian stimulation with a gonadotropin releasing hormone (GnRH) antagonist protocol. DOR patients were selected by the Bologna criteria. The GCs were extracted from the aspirated follicular fluids and RNA isolated from this. The fold change of gene expressions was assessed by real-time polymerase chain reaction (PCR).<br /><strong>Results:</strong> GDF9 expression in patients was 0.23 times lower than the control group, which was significant (P<0.0001). BMP15 expression in patients was 0.32 times lower than the control group, which was significant (P<0.0001). The number of archived oocytes, MII, and two pronuclei (PN) embryos was higher in the control group and these differences were statistically significant (P<0.05).<br /><strong>Conclusion:</strong> Given that GDF9 and BMP15 are specifically involved during follicular recruitmen., we expect expression of these two genes in DOR patients which is greatly reduced by reducing follicular reserve.https://www.celljournal.org/article_253332_17577a2a90d28c9688e6d340f794f9b9.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901Two New Variants in FYCO1 Are Responsible for Autosomal Recessive Congenital Cataract in Iranian Population54655125333510.22074/cellj.2022.8116ENEbrahim ShirzadehDepartment of Ophthalmology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, IranFahimeh PiryaeiDepartment of Molecular Medicine and Genetics, Research Center for Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranHanieh NaddafCore facility of Hamadan University of Medical Sciences, Hamadan, IranZahra Barabadi6517838696, Department of Tissue Engineering, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IranSchool of Medicine, Sabzevar University of Medical Sciences, Sabzevar, IranJournal Article20210622The purpose of this experimental study was to investigate the genetic etiology of congenital cataract (CC) manifesting<br />an autosomal recessive pattern of inheritance in four Iranian families. Affected individuals and their normal first-degree<br />relatives in each family were included in the present study. The genomic DNA of the blood samples was extracted<br />from all participants, and one affected member belonging to each family was subjected to Whole Exome Sequencing<br />(WES). Using bidirectional Sanger sequencing, the identified variants were validated by co-segregation analysis. Two<br />different mutations were detected in the FYCO1 gene encoding FYVE and coiled-coil domain-containing protein. A<br />previously reported missense mutation, c.265C>T (p.Arg89Cys), was found in one Iranian family for the first time,<br />and a combination of two variants in a single codon, c.[265C>T;267C>A] (p.Arg89X), was identified in the three other<br />families. On the other hand, accompanying the c.265C>T mutation, the presence of the c.267C>A polymorphism leads<br />to a premature stop codon. In-Silico Analysis of FYCO1 protein demonstrated that RUN domain will be interrupted<br />so that the large part of functional protein will be eliminated due to this novel variant. FYCO1 has been proved to be<br />involved in human lens development and transparency. Its mutations, therefore, result in CC. Herein, we reported the<br />first autosomal recessive CC patients with c.265C>T (p.Arg89Cys) or c.[265C>T;267C>A] variant in Iranian population<br />for the FYCO1 gene. FYCO1 mutations could be tracked for preventive objectives or even be targeted as therapeutic<br />candidates via treatment approaches in the future.https://www.celljournal.org/article_253335_f170cedf4b0d71be0041ec4f53667c0f.pdfRoyan Institute, Iranian Academic Center for Education Culture and Research (ACECR)Cell Journal (Yakhteh)2228-580624920220901A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova55255425336910.22074/cellj.2022.8444ENHiromitsu TanakaLabaratory of Molecular Biology, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch,
Sasebo, Nagasaki, JapanKeizo TokuhiroDepartment of Genome Editing, Institute of Biomedical Science, Kansai Medical University, Shin-machi, Hirakata City, Osaka, JapanJournal Article20220128HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element<br />(hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studies<br />have shown that Haspin is also expressed in trace amounts in somatic cells; HASPIN also functions in oocytes.<br />Haspin expression is regulated by the tissue-specific methylation of Haspin genomic DNA regions, including somatic<br />cells. This study investigated relationship between 193PE and DNA methylation by examining methylation status of<br />transgenic mice carrying 193PE and a reporter gene. In somatic (liver) cells carrying the reporter gene, 193PE induced<br />methylation as well as trace expression of the reporter gene. In the testis, 193PE induced hypomethylation and intense<br />reporter gene expression. Expression of HASPIN in an egg was assessed using human chorionic gonadotrophin to<br />induce ovulation in female transgenic mice. The results showed that 193PE induced tissue-specific methylation, which<br />resulted in reporter gene expression in a mouse egg.https://www.celljournal.org/article_253369_5059429a9dcb8687172b9df0d42c792e.pdf