Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Volume & Issue: Volume 9, supplement 1, September 2007
250842
EN
Journal Article
2022
03
07
https://www.celljournal.org/article_250842_b25721d796dd0a4824394520998a33a5.pdf
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Assisted Oocyte ActivationUsing Ionomycin Does Not Lead To Subsequent Normal CalciumOscillation Pattern
249749
EN
Heytens
E
Vanhoutte
L
Lierman
S
Cuvelier
C
Gerris
J
Dhont
M
De Sutter
P
Leybaert
L
Journal Article
2022
02
28
<strong>Introduction:</strong> At fertilization sperm triggers oscillations in the cytoplasmic calcium concentration ([Ca+2]i), that are necessary for oocyte activation and normal further embryo development. Artificially raising [Ca+2]i in oocytes injected with round-headed sperm using a Ca+2 ionophore is however sufficient to achieve normal fertilization and embryo development as evidenced by successful human pregnancies and births. However, it is not known yet if assisted oocyte activation can induce the calcium oscillations normally observed after fertilization.
<strong> Materials and Methods:</strong> Mature mouse oocytes collected were loaded with fluo3-AM and injected with round-headed sperm from the consenting patient or sperm from a consenting proven fertile man. Thirty minutes after injection oocytes injected with roundheaded sperm were incubated with the Ca2+ ionophore ionomycin (10μM) for 10 minutes. Changes of fluorescent intensity were recorded immediately after treatment. Extrusion of the second polar body (PB2) and two-cell formation were assessed.
<strong> Results: </strong>None of the oocytes injected with round-headed sperm were activated (0/12) and none of them displayed [Ca2+]i changes. After treatment with ionomycin more than half (42/59) of the round-headed sperm injected eggs were activated and extruded PB2 2 hours later. Ionomycin treatment was associated with a transient [Ca+2]i rise but was not followed by any oscillatory activity over the recording period. By contrast, in almost all of the 19/38 oocytes injected with fertile sperm that extruded PB2, [Ca+2]i oscillations were observed.
<strong>Conclusion:</strong> A single transient rise in [Ca+2]i triggered by ionophore treatment appears to be sufficient to bring about activation of roundheaded sperm injected oocytes, but this does not lead to subsequent [Ca+2]i oscillations. Successful clinical application in the human, however, suggests that this does not seem to prevent the further embryonic development. These experiments thus bring up the question on the role of [Ca+2]i oscillations on embryo development after ICSI with round-headed sperm.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Cryopreservation OfSpermatozoa In Alginic AcidCapsules
249750
EN
Herrler
A
Eisner
S
Bach
V
Weissenborn
U
Beier
H.M
Journal Article
2022
02
28
<strong>Objective:</strong> To develop a method of freezing small amounts of spermatozoa in polymerized alginic acid <font>drop</font>s, which can be liquified after thawing for recovery of the spermatozoa. Design: Prospective clinical study. Setting: Medical School, RWTH Aachen, Aachen Germany.
<strong> Patient(s):</strong> None.
<strong>Intervention(s):</strong> Validation of the encapsulation method with bovine sperm; cryopreservation of human spermatozoa in alginic capsules.
<strong> Main Outcome Measure(s): </strong>We optimized the cryopreservation method by testing different parameters influencing the freezing procedure, such as concentration of alginic acid, size of <font>drop</font>s, time of polymerization, and culture media.
<strong> Result(s):</strong> The final protocol was as follows: encapsulation by 7.3 mg/mL alginic acid forming 10-_L <font>drop</font>s polymerized for 30 seconds and liquefied for 2.5 minutes in sodium citrate. Cryopreservation of human spermatozoa by this protocol resulted in a decreased motility of 18.3% compared with standard protocols but a 19.9% higher vitality of the immotile spermatozoa.
<strong> Conclusion(s):</strong> No difference in viability of spermatozoa after both sperm-freezing procedures could be observed. Further investigation will be undertaken to reduce the amount of immotile but viable sperm after microencapsulation in alginic acid.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ovarian TissueTransplantation: A New Method AndSite For Induction OfFolliculogenesis In Mice As A Modelfor Human Female
249751
EN
Fakhrildin
M.B.A
Al-Rubayae
F.K
Sodani
I.J
Journal Article
2022
02
28
<strong>Introduction:</strong> Ovarian tissue transplantation is a new method of restoring fertility to women whose ovaries are not functioning normally. Young women who undergo chemotherapy or radiotherapy for cancer face serious consequences to their reproductive health and severely affect the ovarian follicular store, especially. Therefore, the aim of this study was to demonstrate induction of the folliculogenesis from ovarian tissue (OT) transplanted under kidney capsule in the presence or absence of gonadotropins support.<br /><strong>Materials and Methods:</strong> Forty eight healthy female mice were anesthetized and abdominal cavity is open. From one side of the body, small piece (~1 X 1 X 1 mm) of OT was transplanted to the subcapsular membrane of kidney at another side, and surgical operation is closed. Then, female mice were classified into three groups according to the time of gonadotropins injection. Group-1: mice injected with sterile normal saline (control group). Group-2: mice injected with gonadotropins directly for four days. Group-3: mice injected with gonadotropins for four days after eight days of surgical operation. Follicular growth, quality of retrieved ova and histological changes for transplanted OT were assessed.
<strong> Results: </strong>In general, no deletion for transplanted OT pieces and no side effects post-operation on mice of all groups were recorded. Best follicular growth of transplanted OT was achieved for groups 1 and 2. Graafian follicles were obtained from transplanted OT of group-2, and less degree for group-1. However, least degree for follicular growth of transplanted OT was reported for group-3 as compared to other groups. Immature and mature oocytes with corona and cumulus cells collected by squashing of transplanted OT.<br /><strong>Conclusion: </strong>The present data demonstrate that the ovarian tissue transplantation is possible to undergo follicular growth subcapsular of the kidney. Also, physiology of the body supports the ovarian follicular growth in another site other than normal position. Further studies are recommended on in vitro Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 26 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 maturation and fertilization of retrieved ova and embryo transfer.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Extract Of Azadirachta Indica(Neem) Leaf Induces Apoptosis InRat Oocytes Cultured In Vitro
249753
EN
Chaube
S.K
Parsad
P.V
Shrivastav
T.G
Journal Article
2022
02
28
<strong>Objective:</strong> To determine whether aqueous neem leaf extract (NLE) could induce degeneration of rat oocytes. If yes, whether apoptosis is involved during NLE -induced degeneration of oocytes cultured in vitro.
<strong>Design: </strong>A controlled prospective study. Setting: Laboratory research setting at Department of Reproductive Biomedicine of the Institute.
<strong> Animal(s):</strong> Fifty four sexually immature female rats that were 24-25 days of age. Intervention(s): The immature female rats were injected with 10 IU pregnant mares serum gonadotropin (PMS) for 48 hrs followed by 10 IU of hCG for 16 hrs. After 16 hrs, rats were euthanized; ovulated cumulus oocyte complexes (COCs) were collected from oviduct. Cumulus-enclosed as well as denuded oocytes were used in the present study.
<strong> Main outcome Measure(s):</strong> Rates of shrinkage, membrane leakage, degeneration, assessment of morphological apoptotic changes, bax protein expression and DNA fragmentation.
<strong>Results: </strong>The NLE induced morphological apoptotic changes such as shrinkage, membrane leakage, cytoplasmic fragmentation prior to degeneration of oocytes. The NLE-treated oocytes that had morphological apoptotic features showed overexpression of bax protein, DNA fragmentation as evidenced by TUNEL positive staining and DNA ladder pattern.<br /><strong> Conclusion: </strong>NLE-induced apoptosis in rat oocytes prior to degeneration in vitro.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Apoptosis In Mouse EmbryosCo-Cultured With Polarized Or Non-Polarized Uterine Epithelial CellsUsing Sequential Culture Media
249754
EN
Azadbakht
M
Rezazadeh Valojerdi
M
Mowla
S.J
Journal Article
2022
02
28
<strong>Introduction: </strong>This study investigated the effects of the in vitro co-culture of mouse embryos with non-polarized or polarized uterine epithelial cells, using sequential culture media, on their development to blastocysts, blastocyst quality (blastocyst diameter and cell number) ,the onset and frequency of apoptosis as well as morphological changes that confirm to the general criteria of apoptosis by using a terminal deoxy nucleaotidyl transferasemediated dUDP nike-end labeling(TUNEL) assay and expression of apoptotic related gene including Bcl-2 and Bax using RT-PCR.
<strong>Materials and Methods:</strong> There were three treatments, all of which used sequential culture media. The treatments were no coculture (control), non-polarized or polarized epithelial cell monolayer co-culture in 24-well tissue culture plates. Mouse uterine epithelial cells were isolated enzymatically and were seeded either on the surface of the culture plate (non-polarized monolayer) or on a Millipore filter insert coated with extra-cellular matrix extract (polarized monolayer) that was then placed in the culture plate. Two-cell mouse embryos were cultured in G-1TMver3 medium to the 8-cell stage when they were randomly assigned to the treatments. The culture medium was G-2TMver3 during the treatment phase of the study. Significances of differences were evaluated by the one way analysis of variance for continuous data.
<strong>Results:</strong> The epithelial cells cultured on Millipore filters became polarized and their morphology compared favorably with those cultured on the surface of the culture plate and in vivo uterine epithelial cells. After 96 h on the treatments, the polarized monolayer had supported the development of significantly more hatched blastocysts (80.0%; p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Three Years Experience WithRoutine Vitrification Of HumanPronuclear Stage Oocytes
249756
EN
Al Hasani
S
Journal Article
2022
02
28
<strong>Introduction:</strong> Cryopreservation of human oocytes and embryos are mandatory tools leading increased cumulative outcome of artifical reproduction techniques while decreasing costs. Vitrification is a cryopreservation technique that leads a glasslike solidification, and rapid freeze of cells and/or tissues. Nowadays it is claimed to be the future of cryopreservation of human embryos due to improved survival rates and clinical outcomes. This study is conducted at a university clinic to asses the safety and efficiency of vitrification of human zygotes as a routine management.
<strong>Materials and Methods: </strong>This study consisted of 106 cycles of cryopreserved embryo transfer (cryoET) and vitrification of 2PN stage zygotes of 82 patients who underwent IVF/ICSI at department of gynaecology and obstetrics, University of Luebeck/Germany between March 2004 and July 2007. Depending on the case additional PN zygotes were prepared for vitrification. The PN zygotes (all zygotes of the same patient together for time saving) were incubated in Equilibration solution (ES) comprising 7.5% Ethylene Glycol (EG) and 7.5% Dimethy Sulfoxide (DMSO) in Ham´s F-10 medium supplemented with 20% serum for 8-10 min (according to the time needed for reexpansion of the zygote) at room temperature. After an initial shrinkage and recovery, they were then aspirated and placed into the Vitrification Solution (VS) (15% EG + 15% DMSO+0.5M Sucrose) in Ham´s F-10 medium supplemented with 20% serum for a period not more than 60sec at room temperature. After having observed that Cytoplasmic shrinkage has been taken place, zygotes were aspirated and placed on the tip of the Cryotop. No more than two zygotes were placed on each Cryotop. Cooling of the zygotes was done by direct contact with fresh clean liquid nitrogen (LN2). The Cryotops were capped under the LN2 to seal and protect the vitrified material before cryostorage for at least two months. Warming of zygotes was performed by placing the Cryotop in Thawing Solution (TS) (1M Sucrose) for a period not more than 60sec. at a temperature of 37 C and then in to Dilution Solution (DS) (0.5M Sucrose) for three min. followed by another DS of 0.25M Sucrose for additional 3 min. at room temperature. The rewarmed zygotes were washed 8-10 times in culture medium before incubation or culture Sage medium under oil for 24 hours prior to embryo transfer. The survival was assessed morphologically at the day of embryo transfer.
<strong>Results: </strong>The mean age of the patients was 32.18 (standard deviation+4.47) and the cause of infertility was either male factor infertility or in combination with tubal infertility who were not complicated with hydrosalpinx. A total of 849 PN stage zygotes were vitrified between March 2004 and July 2007. During this era 103 cycles of cryopreserved embryo transfer were completed. Totally 339 PN zygotes were warmed and warming procedure resulted in a 91.15% survival rate (309 PN zygotes). A three times higher pregnancy rate (36.8%) was obtained when compared to the results of slow rate freezing method (10.2%) which was previously used for a long time in the same centre. In conclusion vitrification of Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 24 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 human zygotes at PN stage seems to be a successful and reliable method with favourable outcomes which can be recommended as a routine technique of cryopreserved of human embryos.
<strong>Conclusion:</strong> Routine vitrification of human zygotes is an efficient and reliable method of cryopreservation of zygotes and seems to improve clinical outcomes of artificial reproductive techniques.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effects Of Varicocele OnChromatin Condensation And DNAIntegrity Of Spermatozoa
249757
EN
Talebi
A.R
Journal Article
2022
02
28
<strong>Introduction:</strong> Varicocele is the most common causes of poor serum production and decreased serum quality.It is demonstrated that patients with varicocele possessed a higher DNA fragmentation index and sperms with nuclear anomalies than healthy fertile men. This may be correlated with an increase of reactive oxygen species in their semen samples. But the effect of varicocele on sperm chromatin condensation is poorly understood. So, the aim of this study is the evaluation of sperm chromatin integrity in these patients.
<strong>Materials and Methods:</strong> sixty men referring to andrology laboratory were categorized into 3 different groups. First a group of 20 infertile patients with varicocele, second a group of 20 infertile patients with abnormal semen parameters .finally a group of 20 fertile men who had normal spermatogram as control group. Semen analysis was performed according to WHO criteria. To evaluate sperm chromatin quality and DNA integrity,Aniline blue, Toluidine blue, Chromomycin A3 and finally Acridine orange stainings were done in all of groups.The slides were analyzed and to determine the percentage of mature or immature sperms, 200 spermatozoa were counted in each slide. The data were analyzed by SPSS(13) software and are presented as mean± standard deviation.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
In Vitro Spermatogenesis
249758
EN
Sofikitis
N
Journal Article
2022
02
28
Induction of meiotic and post-meiotic alterations of male germ cells in vitro has been the target of several research efforts since 1960. However, to date, the establishment of an ideal culture system in which spermatogonial stem cells can be maintained and directed to proliferate and undergo meiosis and complete spermiogenesis does not exist. This is attributed to the difficulties concerning the isolation and purification of defined subpopulations of germ cells and the establishment of male germ cell lines. In addition, there is no adequate knowledge regarding the optimal biochemical conditions that promote the survival and differentiation of germ cells in long-term cultures. This lecture focuses on the methodologies that have been proved sufficient to achieve differentiation of cultured male germ cells. Furthermore, the factors regulating spermatogenesis and the technical prerequisites to achieve differentiation of cultured male germ cells will be described. Finally, the role of in vitro cultures of immature diploid germ cells in the therapeutic management of men negative for haploid cells in their testes and the subsequent potential genetic and epigenetic risks will be discussed.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Experimental Animal Modelsof Varicocele
249759
EN
Sofikitis
N
Journal Article
2022
02
28
Varicocele models can be induced in rats by partially ligating the left renal vein. Induction of left varicocele in Wistar rat results in a bilateral increase in testicular temperature and a bilateral decrease in epididymal sperm content and motility. The above detrimental effects of left varicocele on bilateral testicular spermatogenesis and epididymal sperm maturation process can be counteracted by the administration of factors inducing Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 22 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 testicular vascularization. The administration of indomethasin or hCG to varicocelized rats improves bilateral testicular function. Induction of left varicocele in rabbits has shown that there is a defect in glucose metabolism in the varicocelized testis. This experiment tends to suggest that varicocele is a metabolic disease. Further experiments in varicocelized rats or rabbits have shown that left varicocele affects detrimentally the oxidative status of DNA in sperm nucleus. This may be the reason that embryos developed from the fertilization of oocytes with spermatozoa recovered from varicocelized animals have a lower potential for early development and implantation. Performance of varicocelectomy in experimental animals with left varicocele can ameliorate the detrimental consequences of left varicocele on bilateral spermatogenesis, epididymal sperm maturation, and sperm nucleus oxidative status.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Philosophy Of TesticularDescent
249761
EN
Sofikitis
N
Journal Article
2022
02
28
Mammalian male sex determination is an active process involving complex interactions among several genes. SRY and SOX9 are both responsible for testis formation by initializing and maintaining, respectively, Sertoli cell differentiation. Male sexual differentiation is governed by testicular hormones. Testicular descent (TD) and scrotal evolution occur exclusively but not universally in mammals. Although still debatable, this evolutionarily costly process aims at least to secure lower (than core body) testicular temperatures essential for viable sperm production and storage. TD in scrotal mammals is a multistaged process involving interplay of several anatomical structures and hormonal factors. The gubernaculum appears to play a key role, especially in transabdominal TD (TTD). Androgens and Mullerian inhibiting substance have a rather limited, if any, role during TTD. Leydig cell-derived insulin-like 3-hormone acting directly upon the gubernaculum and proteins encoded by homeobox genes represent good candidate controllers of TTD. Inguinoscrotal TD is mediated by androgens probably acting indirectly upon the gubernaculum, in conjunction with mechanical (abdominal pressure) factors. There is a general agreement on the seasonality of testicular maldescent (TMD) at least in the northern hemisphere. Epididymal malformations, impaired testicular histology due to intrinsic testicular defects, mild hypogonadal state, or increased germ cell apoptotic rate mainly due to abnormal testicular temperature may account for the impaired fertility in individuals with TMD. The theory of an intrinsic testicular pathologic process might plausibly explain the association of TMD with the testicular cancer.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of LIF On LowMotility Sperms In Men WithAsthenospermi
249762
EN
Sajadian
F
Saki
Gh
Kalantar Mahdavi
R.A
Sobhani
A
Journal Article
2022
02
28
<strong>Introduction:</strong> From the past until now extensive study has been taken to deal with the infertility problems in men. Some of the causes are related to men infertility can be defined as reduced number of sperms abnormal morphology and weakness in motility of sperm. Recently improvement in quality of sperm such as number and morphology by in vitro experiments is not possible at the present time. Improvement in quality of sperm motility by means of increasing motility with drugs is possible. Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 21 Investigations have shown that closed relation exist between sperm motility and fertility rate by adding progesterone, platelet activating factor, follicular fluid, cytokines & pentoxyphilline by in vitro experimentation which increasing the ability of the sperm motility and survival time. On the other hand, some studies has been done on the effect of LIF on normal sperms motility and shown that this factor could improve normal sperm motility. In this project by knowing the idea that there is no report about the effect of LIF on low motility sperms in men with asthenospermi, we decided to determine effect of this factor on low motility sperms.
<strong> Materials and Methods:</strong> In this investigation the semen sample of 15 infertile men who have already referred to IVF department of Imam Khomeini Hospital were collected and then preparing them by successive stages for culturing in Ham's F-10 with different concentrations 3, 5, 10, 50ng/ml in incubator on the condition 37 , %5 co2 incubated and samples evaluate for motility and survival time at 6, 24, 48 hours, after culturing, collected data with ANOVA & LSD method by means of SPSS software were analyzed.
<strong>Results: </strong>The results showed that this factor doesn't effect own motility and survival time of sperm with the duration of 6 hours but after 24 hours of culturing in 10ng/ml and 48 hours of culturing in 50ng/ml concentration of LIF respectively the results show improvement in both motility and vitality of sperm.
<strong> Conclusion:</strong> Our data showed that LIF has positive effects on motility and vitalaity of human sperm in dose dependant.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Increased Expression OfInterleukin-1Beta And Interleukin-1? Is Associated With ExperimentalVaricocele
249763
EN
Sahin
Z
Celik-Ozenci
C
Akkoyunlu
G
Turkay Korgun
E
Acar
N
Erdogru
T
Demir
R
Ustunel
I
Journal Article
2022
02
28
<strong>Objective:</strong> To describe the effect of varicocele, in an experimental rat model, on the levels of IL-1β and IL-1 β proteins in testis tissue. Design: Comparative and controlled study. Setting: Experimental research. Animal(s): Wistar male rats in experimental and control groups. Intervention(s): The control group underwent sham operation (n_6). Experimental groups underwent partial ligation of the renal vein to induce experimental varicocele and were then killed at 9(n_6), 11(n_6), and 13(n_6) weeks after induction of varicocele. Main Outcome Measures: Histologic evaluation of the varicocele model was determined by periodic acid-Schiff staining of paraffin-embeded testicular tissues. Levels of cytokines were assessed by mmunohistochemistry and Western blot analysis.
<strong>Results:</strong> Varicocele caused testicular damage, especially in 11- and 13-week-old varicocele groups. In sham-operated rats, Golgi complexes of round spermatids expressed especially the_form of IL-1. By the progression of varicocele, the IL-1_xpression increased temporally in Sertoli cells, spermatogonia, primary spermatocytes, spermatids, and Leydig cells. The expression of IL-1_was seen in Leydig cells in shamoperated rats. The IL-1_ expression was also increased upon progression of varicocele in Leydig cells, Sertoli cells, and spermatogonia.
<strong> Conclusion:</strong> We suggest that IL-1_ and IL-1_ are the regulators of testicular function. Certain pathologic conditions, e.g., varicocele, cause an increase in the expressions of such proinflammatory cytokines. The increased expression of IL-1_ and IL-1_ in varicocele shifts the balance in favor of inflammatory and immune responses and causes detrimental effects in testis tissue, which may cause male infertility.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Cloning And SequenceAnalysis Of A Novel Gene EncodingA Testis Lipid Binding Protein(PERF15) In Human Testis
249774
EN
Pouresmaeili
F
Tehrani
M.K
Journal Article
2022
02
28
PERF15 is a testicular germ cell specific fatty acid-binding protein (FABP) isolated from mammals, originally, rat. It encodes for one of the most abundant proteins of the rat spermatozoa localized in perinuclear theca. It is a small protein of 15 060 molecular weight. Northern blot analysis demonstrated that the rat PERF15 mRNA is transcribed in meiotic and post meiotic cells, exclusively. In this study, we have cloned the human PERF15 gene. For this purpose, we have designed two specific primers to amplify the human PERF15 gene according to the open reading frame of automated computational analysis of Homo sapiens similar to testis fatty acid binding protein 9 with accession #XM_378035. After performance the PCR, a unique band of ~3kb was obtained. Restriction digest using PvuII restriction enzyme, confirmed that the fragment is Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 20 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 related to the same gene. Then we gel extracted the ~3kb band. We sequenced this fragment by direct sequencing using Automatic sequencer (version 3130XL Genetic Analyzer, ABI Applied Biosystem) and 2 specific primers to the gene. Alignment showed 100 % similarity between our gene sequence and the mentioned computational data. The human PERF15 gene contains four exons and three introns. Exon 1 codes for 24 amino acids, exon 2 codes for 57 amino acids, exon 3 codes for 34 amino acids and exon 4 codes for 17 amino acids, respectively. The existing three introns are composed of 2113, 461, and 168 nucleotides. In spite of the homology between exonic regions and exonintron boundaries of the human PERF15 gene and the animal’s (rat& mouse PERF15 genes), the human PERF15 gene is different in size and sequence of the corresponding introns with rat and mouse PERF15.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Role Of Cyclooxygenases InMale Reproduction
249775
EN
Pallu
R
Reddy
P.R.K
Reddy
G.V
Roy
R
Mallikarjuna Reddy
M
Sreenath
A
Neeraja
S
Subhashini
J
Mahipal
S.V.K
Reddy
M.C
Journal Article
2022
02
28
Arachidonic acid, the major plolyunsaturated fatty acid (PUFA) in cell membrane phospholipids of mammalian systems, is mainly oxygenated by two important pathways, the cyclooxygenase (COX) pathway to generate prostaglandins, thromboxanes and prostacyclin and the lipoxygenase (LOX) pathway to generate leukotrienes and lipoxins. These oxygenated metabolites, collectively termed as eicosanoids, are extremely potent biologically active molecules with bewildering variety of actions. There is no system in the body that is not effected by eicosanoids in one way or the other. In the present study we are working in understanding the role of cyclooxygenases in reproduction. Cyclooxgenase, the rate limiting enzyme in the production of Prostaglandins, exitsts in two isoforms, the constitutive cyclooxygenase-1 (COX-1) and the inducible cyclooxygenase-2 (COX-2). Even though COX-2 is known broadly as the inducible isoform expressed in response to inflammatory and mitogenic stimuli. We have demonstrated for the fisrs time the consitutive expression of COX-2 in rat testis. The COX-2 mRNA in the testis is smaller (2.8 kb) than that of the inducible COX-2. Hormone treatment (Testosterone/follicle stimulating hormone) regimes increased the levels of COX-2 protein suggesting that sustained levels of COX-2 protein in testis can be influenced by gonadotrophins and androgens. A novel functional association of rat testicular GSTs with cyclooxygenases in vitro was demonstrated. These studies reveal a reversible functional interaction between membrane-associated cytosolic glutathione Stransferases (mac GSTs) and COX in vitro, with possible interactions between them at GSH binding site. It is proposed that the constitutive COX-2 may be involved in spermaogoneal renewal. Also the role of COX-2, iNOS and oxidative stress in male infertility during endotoxin-induced acute inflammation was elucidated. Inflammationinduced COX-2 was shown to decrease steroidogenic acute regulatory (StAR) protein levels and thus affect testosterone biosynthesis and stermatogenesis in rats.These studies have lead to the basic understanding on the role of constitutive and inducible isoforms of COX-2 in male reproduction.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Effects Of Different Doses OfHyaluronan On Human SpermMotility, Vitality And Morphology
249776
EN
Mohammadi
Roushandeh A
Sobhani
A
Sohrabvand
F
Amanpour
S
Pasbakhsh
P
Salehi
M
Sargolzaei
F
Journal Article
2022
02
28
Background: Important aspect of sperm function such as motility and capacitation appear to be mediated at least partially though hyaloronic acid (HA). The present study investigated effects of different doses of hyaluronan on sperm motility, vitality and morphology in human. Materials and Methods: Motility, vitality and morphology of raw semen was analyzed according to WHO criteria before and 4 hour after treatment with different doses of hyaluronan (0, 750, 1000 and 1250μg/ml). Results: The results of present study showed in the group treated with 1000 μg/ml hyaluronan compare to control group there was an increase in the percentage of stages 3 and 4 but a decrease in the number of stages 1 and 2. In the group treated with 1250 μg/ml stages 1 and 2 increased while stages 3 and 4 decreased. Vitality in all groups decreased except of the group treated with 1000 μg/ml hyaloronan. The group decreased. With 1250 μg/ml hyaloronan showed significantl Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 19 decrease in vitality compared to fresh group (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ultrastructural Study OnApoptotic Effects Of Myleran InSpermatogenic Cells In Adult MiceTestis
249777
EN
Mohammad Ghasemi
F
Soleimani Rad
J
Ghanbari
A.A
Journal Article
2022
02
28
<strong>Introduction: </strong>Myleran is known to have testicular toxicity leading to male infertility. Little is known about ultrastructural effects of myleran on male germ cells. In this study, we examined the possible involvement of apoptosis in the induction of germ cells degeneration following treatment with different doses of myleran using transmission electron microscopy (TEM) and light microscopy (LM).
<strong>Materials and Methods:</strong> At the present study 8 weeks old NMRI mice were divided in 5 groups. Control animals were treated with DMSO. However the other animals in the 2nd, 3rd, 4th and 5th groups were treated intraperitoneally with different doses of myleran: 5, 10, 20 and 40 mg/kg respectively. All animals were dissected after35 days and their testis was evaluated for detection of apoptosis using TEM and LM.
<strong>Results:</strong> In all myleran treated groups except in 2nd group, the nucleous of spermatogonia was marginal hyperchromatin with a crescent form. Primary spermatocytes showed cellular shrinkage and vacuolization inside the cytoplasm. Presence of large spaces between adjacent cells was another sign. Few round spermatids were affected and all signs were increased with the increasing of the dose. In LM study spermatogonia numbers were significantly reduced in the 4th and 5th groups (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Reactive Oxygen Species(ROS) Level In Seminal Plasma OfInfertile Men And Healthy Donors
249778
EN
Moein
M.R
Tabibnejad
N
Dehghani
V
Vahidi
S
Journal Article
2022
02
28
<strong>Introduction:</strong> Reactive oxygen Species (ROS) are a group of free radicals that in excessive amounts showed to have negative influence on sperm quality and function. Some Clinical entities such as varicocele, spinal cord injury and genital infections showed to be associated with high ROS levels in semen.In this study we assessed ROS levels in seminal plasma of infertile men and compare with its level in healthy donors. We also determined the ROS level in semen of infertile patients according to the etiology of infertility, and also the effect of smoking on its level.
<strong>Materials and Methods:</strong> We selected 95 infertile patients and 63 healthy donors as control. Complete physical examination, semen analysis, scrotal sonography and hormone assay were done for all patients. Azoospermic patients were excluded from the study. ROS level in semen was measured by chemiluminescence assay in both groups. Patients also divided in two groups according to the etiology of infertility.
<strong>Results:</strong> We were excluded 32 infertile patients from the study. Semen specimens from 126 individuals, 63 samples from infertile men and 63 from healthy controls were studied. The mean age of normal subjects and infertile men were 30.78±3.73 years and 31.43±6.60 years respectively. The mean Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 18 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 ROS level in normal men was 180.05 RLU, and 1852.04 RLU in infertile patients, which is significantly higher in case group (p=0.000). Fifty patients had varicocele and for the other 13 patients no any specific etiology was found. The mean ROS level in varicocele patients was 2215.42 and in unknown group was 454.44 (p=0.048).
<strong>Conclusion: </strong>Our study showed that level of ROS in seminal fluid of infertile men is significantly higher than fertile donors and also in infertile patients with varicocele is higher than patients with unknown cause.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Expression Of StressInducible Protein 1 (Stip1) In TheMouse Testis
249779
EN
Mizrak
S.C
Bogerd
J
De Rooij
D.J
Del Mazo
J
Parraga
M
Lopez-Casas
P.P
Journal Article
2022
02
28
Phthalate esters are considered endocrine disruptors that interfere with the endocrine balance and development of the mammalian testis. Mono-2-ethylhexyl phthalate (MEHP), the active metabolite of the ubiquitously used plasticizer di-2-ethylhexyl phthalate (DEHP), acts upon Sertoli cells as initial target. By subtractive cDNA libraries we identified genes deregulated as response to MEHP in primary cultures of mouse Sertoli cells. The expression of mouse stress inducible protein 1 (Stip1) was detected as upregulated as a result of MEHP exposure. Stip1 is a cochaperone protein that is homologous to the human heat shock cognate protein 70 (hsc70)/heat shock protein 90 (hsp90)- organizing protein (Hop). To assess the presence and localization of Stip1 in mouse testis and its potential role in stress defense, we studied the expression pattern of the Stip1 protein by immunohistochemistry and of the mRNA by in situ hybridization. Both the protein and the mRNA of Stip1 were mainly found in the cytoplasm of all types of spermatogonia and spermatocytes up till zygotene, the expression decreased during late pachytene and was very weak in diplotene spermatocytes and round spermatids. Interestingly, this expression pattern resembled the pattern of stress sensitivity of spermatogenic cells in that the most sensitive cell types show the weakest expression of Stip1. This suggests an important role for Stip1 in the ability of germ cells to survive in stress conditions including high temperatures.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Origin, Localization AndBinding Abilities Of Boar DQHSperm Surface Protein Tested BySpecific Monoclonal Antibodies
249780
EN
Ma??skov?
P
P?knicov?
J
Jon?kov?
V
Elzeinov?
F
Tich?
M
Journal Article
2022
02
28
<strong>Introduction:</strong> Seminal plasma proteins bind the sperm surface at ejaculation and may modulate several aspects of sperm activity during reproduction. DQH sperm surface protein, present in boar seminal plasma, shows affinity to phoshorylcholine, acidic polysaccharides, oviductal epithelium and zona pellucida glycoproteins.
<strong>Materials and Methods:</strong> Monoclonal antibodies (MAbs) against DQH protein were prepared and used for determination of the DQH protein origin in boar reproductive organs, its localization on boar spermatozoa, and for investigation of its binding abilities in the porcine oviduct and to the zona pellucida of the oocyte.
<strong>Results: </strong>The mRNA transcript of DQH protein was found in seminal vesicles, not in the testis, epididymis and prostate. Its translated products were immunodetected by MAbs in Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 17 seminal vesicle extract and fluid, on seminal vesicle tissue sections and on the membraneassociated acrosome part of ejaculated spermatozoa.
<strong>Conclusion: </strong>These results confirmed the ability of DQH protein to bind the sperm surface at ejaculation and to participate in the formation of the sperm reservoir in the porcine oviduct. Moreover, monoclonal antibodies reduced binding of sperm to oocytes and proved the role of DQH protein in the spermzona pellucida primary binding.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Demonstration Of Follicle-Stimulating Hormone Receptor InCauda Epididymis Of Rat
249781
EN
Lekha Dahia
C
Rao
A.J
Journal Article
2022
02
28
FSH receptor has been shown to be specifically expressed only in the Sertoli cells in males. In one of our studies that consisted of deprival of endogenous FSH in immature rats and adult bonnet monkeys, atrophy of the epididymis was observed, cauda region being the most affected. Although epididymis is an androgen-dependent tissue, the changes in histology of the cauda region were observed without any associated change in the levels of testosterone in FSH-deprived animals. Considering this, it was of interest to evaluate the possibility of epididymis being a direct target for FSH action. In the present study, we have examined the expression of FSH receptor in the epididymis of rat and monkey. In the cauda region of rat epididymis, FSH receptor expression was demonstrated by RTPCR and Northern and Western blot analyses. FSH receptor was found to be functional as observed by its ability to bind 125IoFSH, by an increase in cAMP production, and by BrdU incorporation following addition of FSH under in vitro conditions. These results suggest the possibility of a role for FSH in regulating the growth of the epididymis. Episismis, follicle-stimulation hormone, follicle-stimulating hormone receptor, male reproductive tract.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Effect Of L-Ascorbic AcidSupplementation On TesticularOxidative Stress And EndocrineDisorders In Mature Male RatsExposed To Intensive SwimmingExercise
249782
EN
Jana
K
Samanta
P.K
Manna
I
Ray
B.R
Journal Article
2022
02
28
In order to investigate the ameliorative potential of L-ascorbic acid on intensive swimming exercise induced testicular oxidative stress, 18 Wistar male rats (age: 3 months, weighing: 127.5±5.3g) were randomly divided into: 1) control group (CG, n=6); 2) experimental group (EG, n=6); 3) supplemented group (SG, n=6). An exercise protocol of 3 hour swimming/day, 5 days/ week was followed for 6 weeks in EG and SG with no exercise in CG. In SG L-ascorbic acid was supplied orally at a dose of 25-mg/kgbody weight/day for 6 weeks. A significant diminution (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparative Effect OfAminoglycosides (Gentamycin,Neomycin, Streptomycin) AndFluoroquinolones (Ofloxacin)Antibiotics On Sperm Parametersand Testis Apoptosis In Rats
249783
EN
Khaki
A
Ghaffari Novin
M
Heidari
M
Bazi
P
Nouri
M
Journal Article
2022
02
28
<strong>Introduction:</strong> Aminoglycosides (Gentamycin, Neomycin, Streptomycin) and fluoroquinolones (ofloxacin) are synthetic antibacterial agent antibiotics with a very broad spectrum against microbial pathogens, especially the Gram-negative. The aim of this study was to investigate the comparative effects of these drugs on testis apoptosis and sperm parameters in rats.
<strong>Materials and Methods:</strong> The fifty male Wistar rats were selected and randomly divided into control (n=10) and experimental (n=40) groups. The experimental groups split into four groups. First, second, third and fourth experimental groups received 5 mg/kg (IP) Gentamycin, 50mg/kg (IP) Neomycin, 40mg/kg (IP) Streptomycin and 72mg/kg (PO) ofloxacin daily for fourteenth day, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and sperm removed from cauda epididymis and analyzed for sperm motility, morphology, and viability. Testis tissues were also removed and prepared for TUNNEL assay for detection of apoptosis.
<strong>Results:</strong> There was a significant decrease in sperm count, viability and motility in all of experimental groups when compared with control group (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Raised InflammatoryMarkers In Semen From Men WithAsymptomatic Chlamydial Infection
249784
EN
Kokab
A
Eley
A
Akhondi
M
Sadeghi
M.R
Modarressi
M.H
Jennings
R
Pacey
A.A
Journal Article
2022
02
28
<strong>Introduction:</strong> Chlamydia trachomatis infection is the most common bacterial sexually transmitted infection throughout the world. In females infections may lead to tubal factor infertility, pelvic inflammatory disease, salpingits and ectopic pregnancy. In men, it is associated with a wide clinical spectrum which may lead to infertility. Both males and females can suffer asymptomatic infection. C. trachomatis infections can be transmitted from either symptomatic or asymptomatic males or females to the opposite sex. The way to diagnose these symptoms less infections is by laboratory testing. The aim of this study was to evaluate the relationship between the presence of Chlamydia trachomatis and inflammatory markers in semen from males of infertile couples.
<strong>Materials and Methods:</strong> Concentrations of leukocytes, interleukin (IL)-8 and IL-6 were determined in seminal plasma (SP) from 255 male partners of asymptomatic infertile couples undergoing diagnostic semen analysis as part of ongoing infertility investigations. Semen analysis was performed according to WHO (1999) methods. In addition, strand displacement amplification (SDA) and polymer conjugate enhanced enzyme immunoassay (IDEIA PCE) were undertaken on semen and first-void urine samples to detect the presence of C. trachomatis-specific DNA and Chlamydia genus-specific antigen respectively. Nested plasmid PCR and Direct Immunofluorescence (DIF) were used to confirm positive SDA and EIA. Sperm motility and viability were assessed both initially and after three hours incubation at 37°C. Results: A total of 14 men (5.5%) were found to meet our criteria of genital chlamydial infection by having at least two positive samples (semen and urine) and/or two positive tests (SDA and IDEIA PCE) for Chlamydia. Men with chlamydial infection had a significantly (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Limitations Of The SemenAnalysis And The Importance OfDiagnosis In The Evaluation OfInfertility In The Male Patient
249785
EN
Jequier
A.M
Journal Article
2022
02
28
Semen analysis is used both as an indicator of infertility as well as a basis for treatment. However a semen analysis gives poor discrimination between infertility and fertility and is only of real value in indicating infertility when the variables within the semen analysis are at their extremes. The reason that a semen analysis is a poor discriminator of fertility is clear as the difference between a conception and a non conception cycle involves one single sperm: in a conception cycle all sperm are lost while in Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 14 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 a conception cycle all but one sperm are lost. Therefore the number of motile and morphologically normal sperm can only give the clinician a probability of pregnancy. Thus wether the count is 10% more or 10% less can make little difference clinically. For this reason accurate sperm counts are unnecessary in clinical andrology. Only when all the sperm in an ejaculate show an abnormality can a semen sample truly be said to be infertile. One important aspect of infertility management is to arrive at a clinical diagnosis. Only with a clinical diagnosis can both the pathophysiology underlying patients infertility and the generation of an abnormal semen analysis be determined and understood. Only with an understanding of the causation of infertility can any preventative measures be taken, can any rational treatment be provided and the frequently unwarranted use of IVF be avoided. In the male, treatment often relates solely to the sperm count and not its underlying cause. Much of the causation of male infertility in clinics today goes undiagnosed. In my recently completed (but unpublished) study of testicular ultrasound among a group of 749 men attending an infertility clinic, a large of amount, much of male infertility will remain misunderstood.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison BetweenReactive Oxygen Spieces (ROS)Concentration In Seminal Plasmaand Semen Parameters In Partnersof Patients Who Became Pregnantafter IVF/ICSI And Those Who DidNot
249786
EN
Hammadeh
M.E
Radwan
M
Al-Hasani
S
Lorenz
M
Schmidt
W
Journal Article
2022
02
28
The aim of this study was to determine and compare the level of ROS in seminal plasma and other sperm parameters (count, morphology, membrane integrity, maturity, DNA-fragmentation) in order to find out the relationship between ROS concentration and spermatozoa quality and their effect on fertilization and pregnancy of patients who became pregnant and those who did not after IVF/ICSI treatment. 26 IVF and 22 ICSI patients were included in this study, the ROS level in seminal plasma and sperm concentration, vitality (Eosin-test), motility, morphology, membrane integrity (HOS-test), maturity (Chromomycine CMA3) and DNA Fragmentation (TUNEL) results and their relationship to fertilization and pregnancy were analysed. ROS concentrations were at the same level in the seminal plasma of male partner of patients who became pregnant and those who did not. The other semen parameters, concentration, motility, vitality, membrane and DNA integrity were similar in both groups. However, in both groups a negative correlation could be found between ROS concentration and sperm vitality, membrane integrity and morphology. Besides an inverse correlation, could be found between TUNEL, vitality, and membrane integrity. In Conclusions: ROS concentration in seminal plasma affects spermatozoa’s quality but can not affect the fertilization rate in IVF/ICSI programme.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of SemenParameters And Sperm ChromatinStatus Before And PostVaricoclelctomy On Fertility
249787
EN
Haft Baradaran
B
Nasr Esfahani
M.H
Razavi
Sh
Haji Mirza Alian
F
Sadeghy
M
Abasi
H
Journal Article
2022
02
28
<strong>Introduction:</strong> Different methods have been used to evaluate the beneficial effect of varicocelectomy; these include semen parameters and pregnancy rate. Due to high biological variability of semen parameters, sperm functional tests have been considered as an efficient end point in assessment of fertility. Therefore, the aim of this study was to evaluate the effect of varicocelectomy on semen parameters, sperm chromatin and membrane integrity.
<strong>Materials and Methods:</strong> In 35 patients over a 2 year period protamine deficiency, presence of excessive histone, chromatin stability, Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 13 ability of sperm to undergo decondensation and membrane integrity were assessed by chromomycin A3, aniline blue staining , SDS, SDS+EDTA and HOST, respectively.
<strong>Results:</strong> The results of this study showed that among semen parameters only sperm motility, sperm membrane integrity and sperm chromatin integrity showed significant improvement post surgery. The cumulative pregnancy rate in this was 25.7%. Comparing the results of the aforementioned parameters between patients whom became pregnant compared to those who did hot benefit from varicocelectomy show that patient may benefit from varicocelectomy that have improved sperm morphology, decreased sperm protamine deficiency and decreased presence of excessive histone at 3 month post surgery (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison Of The Number OfSpermatogonia And Sertoli Cells InFetal And Neonatal TestesAutopsied Between 1958-1964 And1989–1998 In Tokyo
249788
EN
Takemura
T
Endo
H
Fukayama
M
Tsutsumi
O
Journal Article
2022
02
28
<strong>Background:</strong> Studies in several countries have reported a decline in human sperm quality similar to that observed in wild animals. To quantify whether the number of sperm in humans has decreased and whether humans are affected by similar environmental influences, we compared the number of spermatogonia and Sertoli cells in human fetal and neonatal testes autopsied at two institutions in Tokyo between 1958–1964 (term A) and 1989–1998 (term B), with special attention to chronological changes during gestation.
<strong> Materials and Methods:</strong> We used an immunohistochemical method with antibody against neuron-specific enolase to determine the percentage of seminiferous tubules containing spermatogonia in the formalin-fixed tissue samples, and a morphometrical method using a dissector to count the number of spermatogonia.
<strong>Results: </strong>There were no significant statistical differences between the two time periods in the regression parameters compared for the number of spermatogonia and Sertoli cells, nor was there a remarkable difference in the estimated number of Leydig cells.
<strong>Conclusion:</strong> The results indicate that even if there has been a deterioration in human semen quality, it is not necessarily caused by endocrine disruption of fetal testicular development.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Correlation Between SeminalPlasma Glutathione PeroxidaseEnzyme Activity And SemenParameters
249789
EN
Eidi
M
Pouyan
O
Eidi
A
Fazaeli
R
Shahmohammadi
P
Dadgar
M
Journal Article
2022
02
28
<strong>Introduction:</strong> Sperm cell membranes are susceptible to peroxidative damage by an excess of reactive oxygen species (ROS). Antioxidative defence systems consisting of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) physiologically control the balance between ROS production and neutralization.
<strong> Materials and Methods:</strong> In the present study, correlations between seminal plasma glutathione peroxidase enzyme activity and Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 12 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 semen parameters are evaluated in 240 males. Semen analysis was performed according to World Health Organization guidelines. The 240 males were subdivided into 5 groups as normospermia, oligozospermia, asthenozospermia, azospermia and varicocele according to their spermiogrames. Seminal plasma glutathione peroxidase enzyme activity was determined by Kit (Randox, Germany).
<strong>Results: </strong>The result showed that glutathione peroxidase enzyme activity is higher in normospermic than oligozospermia, asthenozospermia, azospermia and varicocele groups. Also, there are significant and negative correlations between glutathione peroxidase enzyme activity and seminal plasma fructose concentration, white blood cell, tail defects of sperm, coiled tail sperms and short tail sperms. On the other hand, the present data showed that significant and positive correlations between vitality, sperm count, motility and normal morphology.
<strong>Conclusion: </strong>So, the present study showed that measurement of glutathione peroxidase enzyme activity could be a good marker for evaluation of male infertility.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Hyaluronan Binding Protein(HABP1) As Diagnostic Marker ForMale Infertility And Its Use In IVF
249790
EN
Datta
K
Chand Thakur
S
Ghosh
I
Bharadwaj
A
Journal Article
2022
02
28
The lack of effective diagnosis of testicular disorders leading to infertility is a major problem in reproductive biomedicine. A better understanding of male infertility related to abnormal spermatogenesis associated with differential gene expression is of interest. We are working on a novel hyaluronan binding protein (HABP1) that interacts specifically with HA and facilitates HA mediated processes including sperm-oocyte interaction1 and sperm motility2. Sequence analysis of HABP1 from human fibroblasts reveal its identity with SF2/p32, the protein copurified with alternate splicing factor3 and globular head of C1Q thus represented in human Chromosome 17p13.34 as synonym HABP1/p32/C1QBP. HABP1 is synthesized as a proprotein which forms mature protein by cleavage of initial 73 amino acids. This proprotein form is extremely labile and detected only in pachytene spermatocytes and round spermatids in germ cells in adult testis5. Further analysis demonstrates that though mature form of HABP1 is present in the testis, its precursor form was not found in testis of 7, 14, 21 and 28 day old rat, but is present only in pachytene spermatocytes and round spermatids of testis of 21 day and 28 day old rats. With spermatogenic arrest, HABP1 is lost from pachytene and round spermatids suggesting that the expression of HABP1 proprotein may be crucial for spermatogenesis6. Earlier we reported on the reduction in the level of HABP1 on sperm surface in asthenozoospermic and oligospermic patient7. In continuation, we have also demonstrated the interaction of HABP1 with zona pellucida of buffalo and shown that the supplementation of IVF medium with rHABP1 can promote the capacity of sperm binding to oocytes under invitro fertilization conditions even in presence of D-mannosylated albumin (DMA), known to inhibit sperm oocyte interaction8. Thus we propose to use HABP1: 1. as a diagnostic marker for male infertility and spermatogenic arrest in testicular biopsy. 2. in IVF medium to promote sperm oocyte interaction.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Signal TransductionMechanisms Involved In In VitroRam Sperm Capacitation
249791
EN
Blanco
M.T.M
Grasa
P
Cebri?n Pérez
J
Journal Article
2022
02
28
This article represents a step forward in our study on ram sperm functioning and the relationship with fertility. Artificial insemination in sheep has not been widely adopted, probably due to the low fertility rate obtained with frozen-thawed semen, possibly due to premature capacitation-like changes. Therefore, a good knowledge of the sperm capacitation process could help in the formulation of better diluents that prevent these changes during sperm freezing or storage, and, therefore, improving the sperm fertilizing capacity. In previous studies we reported that in vitro capacitation and acrosome reaction induced a decrease in the content and the redistribution of P14 and P20, two ram seminal plasma proteins that protect spermatozoa against coldshock. Our results suggested that the protective effect of these proteins could be related to their decapacitating role (JAndrol2005). Likewise, we showed that membrane protein tyrosine phosphorylation is related to the capacitation state of ram spermatozoa (MRD2001). However, there had not been any report about the molecular regulation mechanism of this process in ram. Therefore, in this study we investigated basic aspects of the signal transduction pathways that are activated during capacitation. Our results demonstrated that in ram sperm, capacitation and the associated protein tyrosine phosphorylation is not absolutely dependent on the presence of BSA and calcium, and that the PKA/cAMP pathway is, at least, partially implicated in the tyrosine phosphorylation of some proteins. Our data indicate that the signal transduction mechanisms of capacitation in ram sperm differ from those in other mammals, which suggests that species specificities might exist Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 11 with respect to this process. Our findings might benefit our understanding of the biochemical mechanisms involved in mammalian sperm capacitation and ultimately, fertility. Furthermore, in this article we validate the chlortetracycline (CTC) technique for the assessment of ram sperm capacitation state, performing a specific determination in viable cells exclusively.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Modern Management Of MaleInfertility
249792
EN
Baker
H.W.G
Journal Article
2022
02
28
Males suspected to be infertile should have a detailed medical history and physical examination. Semen analysis is the main investigation. Unless the clinical picture is clear, several semen analyses need to be done because of the day-to-day variability. Measurement of gonadotrophin and Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 10 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 testosterone levels is helpful in distinguishing primary from secondary testicular failure. Testis biopsies are useful for confirming obstructive azoospermia and determining the type of spermatogenic defect with primary seminiferous tubule disorders. Other investigations: karyotype, genetic tests for Yq microdeletions, cystic fibrosis, imaging for pituitary tumour or ejaculatory duct obstruction, are performed where indicated. A number of conditions are untreatable and cause sterility, in particular primary spermatogenic disorders where no live sperm are produced. These patients need to consider other alternatives for having a family by donor insemination or adoption. Over the last 15 years it has become clear that sperm or elongated spermatids that can be used for intracytoplasmic sperm injection (ICSI) may be found in a proportion of patients with severe testicular disorders such as Klinefelter syndrome and Sertoli cell only syndrome, either in the semen or in testis biopsy specimens. Conditions that may be treatable to increase the chances of natural conception include: gonadotropin deficiency or suppression, sperm autoimmunity, genital tract obstruction and reversible toxin exposures or illness effects and some coital disorders. However ICSI is often a better alternative for conditions such as sperm autoimmunity and genital tract obstruction. The remaining patients have less severe abnormalities of the semen, ranging from oligospermia to normal standard semen analyses but defective sperm function. These patients may have varicoceles, features of low grade genital tract inflammation, increased abnormal DNA in the sperm and increased production of reactive oxygen species by their sperm. There are no good controlled trials which prove treatment of these problems will increase natural conception rates. These patients are subfertile rather than sterile and pregnancies may occur but at lower than normal rates. Infrequent or mistimed coitus and female factors such as ovulatory disorders may be contributing. Thus the male and female partners should be treated as a couple and reversible factors treated where possible. The estimation of prognosis for natural conception is also important. If this is low, ICSI is usually effective.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Sperm-OocyteInteraction Testing
249793
EN
Liu
D.Y
Baker
H.W.G
Journal Article
2022
02
28
During natural conception and standard in vitro fertilisation (IVF), motile capacitated sperm with intact acrosomes bind to the surface of the zona pellucida (ZP) and this binding triggers the acrosome reaction (AR). The sperm then passes through the ZP and binds to the oolemma via the plasma membrane that persists over the equatorial segment. The sperm is then engulfed into the ooplasm where decondensation of the nucleus occurs to form the male pronucleus. We have developed tests for human sperm- ZP binding, the ZP-induced AR and spermoolemma binding using oocytes which failed to fertilise in the clinical IVF program. The patients consent to the use of this material for testing or research. Usually the oocytes maintain their ability to bind sperm and stimulate the AR. The ZP can be preserved in concentrated salt solution at 4oC for months. Using these assays we have found defective sperm-ZP binding and disordered ZP-induced AR are common causes of failure of IVF when there are sperm defects, but can also occur with normal semen analysis. These defects of sperm-oocyte interaction could account for about 25% of patients with idiopathic infertility and if diagnosed before IVF is attempted would allow the patients to be treated by ICSI and avoid an IVF cycle with low or zero fertilisation. In contrast, oolemma binding defects appear to be rare. Using experimental conditions in which the amount of ZP was not limiting, we showed that only a small proportion
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Semen Analysis-WHO Manual5th Edition And The Future OfAutomation
249794
EN
Clarke
G.N
Garrett
C
Liu
D.Y
Journal Article
2022
02
28
WHO has made a significant contribution by producing successive revisions of the semen analysis manual. The 5th edition to be published soon has a number of improvements with clearer and simpler instructions for standard analysis particularly for sperm concentration and motility. Morphology continues to be done by the strict method. There are tables for comparing duplicates. New reference values (weighted 5th percentiles) from about 1600 fertile men (recent fathers) from different countries are included. The section on sperm preparation has been expanded to cover common methods of isolation of sperm for assisted reproductive technology. A chapter on sperm cryopreservation has been added. The quality control chapter has been rewritten. There is less material in appendices but there is a new appendix on the basics of microscopy. The aim of the manual is to standardise semen analysis but experience with external quality assurance indicates this is difficult to achieve. This is particularly the case with sperm morphology. Clinicians will continue need to know the reference ranges for their own laboratories. We have experience with routine use of computer assisted semen analysis (CASA) for sperm concentration, percent progressively motile sperm and sperm kinematics: straight line velocity (VSL), using the Hamilton Thorn CASA with IDENT fluorescent staining of the sperm. This requires dilution of samples with high sperm Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 9 concentration with seminal plasma and selection of fields across the counting chamber to achieve accuracy. For sperm concentrations above 2x106/mL the quality control is very good. We have a sperm morphometry system based on automated focusing and slide movement that provides 32 morphometric measurements of the sperm head and upper neck regions including features related to density of staining that allow orientation of the sperm head and assessment of the acrosomal region. An index (%Z) derived from features selected by the sperm-ZP binding process was related to natural conception rates in about 1200 subfertile couples. VSL and female age were also significant in regression analysis models. We believe these and other advances in CASA will greatly improve semen analysis.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Role Of Color DopplerUltrasound In Prediction Of TheOutcome Of MicrosurgicalSubinguinal Varicocelectomy
249795
EN
Alayman
H
Journal Article
2022
02
28
<strong>Objective: </strong>Over-diagnosis and under treatment of varicocele may be responsible for poor outcome of varicocelectomy. In this study, we used Color Doppler ultrasound for accurate diagnosis and grading of varicocele and to predict the outcome of microsurgical subinguinal varicocelectomy.
<strong>Materials and Methods:</strong> A total of 104 patients undergoing microsurgical subinguinal varicocelectomy for treatment of infertility were included in this study. Patients were evaluated with routine history, physical examination, semen analysis, hormonal assessment and scrotal ultrasound and Doppler. After varicocelectomy, improvement index, in sperm concentration, was calculated by dividing the difference between the postoperative and preoperative sperm concentration by the preoperative sperm concentration. Improvement Index greater than 0.5 is considered a good outcome. Statistical analysis was done to study the correlation between microsurgical varicocelectomy outcome and testicular vein diameter at the inferior pole of the testis and the degree of reflux measured by color Doppler ultrasound.
<strong>Results:</strong> Improvement index in sperm concentration, motility and morphology more than 0.5 was achieved in 58.8%, 27.3% and 17.6% of cases respectively. We found that patients with testicular vein diameter, at the inferior pole of the testis, more than 2.5 have significantly higher improvement index in sperm concentration, motility and morphology than in patients with testicular vein diameter less than 2.5mm (p=0.006, 0.016, 0.041 respectively). We also found that patients with clear reflux detected by color Doppler ultrasound at the inferior pole of the testis have a significantly higher improvement index in sperm concentration, motility and morphology than patients with reflux detected only in the supratesticular venous channels (p=0.013, 0.015 and 0.045 respectively).
<strong>Conclusion: </strong>Color Doppler ultrasound is a useful tool for accurate diagnosis and grading of varicocele and to predict the outcome of varicocelectomy. We recommend doing varicocelectomy in cases of testicular vein diameter more than 2.5mm and in cases of reflux detected at veins at the lower pole of the testis.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-86: Endometrial Inflammatory Factors And Embryonic Implantation
249796
EN
Farina
M
Journal Article
2022
02
28
Chronic endometritis (CE) represents a chronic inflammation of the endometrial lining. In most of cases diagnosis is casual on histological specimen taken for different gynecological indications (AUB, infertility etc.). CE in most of cases is asymptomatic or accompanied by mild disturbances like spotting or bleeding (94% of cases), mild and undefined pelvic pain, mild leucorrhoea. Its prevalencerange from 0.5 to 15% in general population, while is 70% in women referring to centres for sexual transmitted diseases, often representing an hidden pathology. The gold standard for the clinical definition and diagnosis of CE is represented by histology. The diagnosis of chronic endometritis relies on identification of plasma cells in the endometrial stroma. Lymphocytes, neutrophils, histiocytes, and eosinophils, are generally not diagnostic for endometritis, because they are normal constituents of the stroma. Inflammatory cells may infiltrate the wall of glands and may concentrate around vessels. The inconvenient factor is that these aspects may normally be present at menstruation and so diagnosis at histology may be missed. As regards the pathophysiology, an abnormal uterine bleeding (AUB) can be observed from 70 to 90% of cases in which histology results positive for CE (Greenwood, 1995), accompanied by symptoms such as chronic pelvic pain, infertility and, less frequently, leucorrhoea and/or fever. In a recent Italian study it was reported that the indication at hysteroscopy in 438women diagnosed with CE and 100 controls was 42% and 22% for dysfunctional uterine bleeding, respectively for CE and controls; 10% and 38% for positive transvaginale ultrasonography for endometrial polyp; 36% and 8% for infertility; 6% and 23% for submucous myoma and only 4% and 19% for mullerian abnormalities. These results show the significative relation between CE and AUB, and between CE and infertility. According to the pathophysiology of the endometrial bleeding, in accord with the studies by Ferenczy, during a normal menstruation, in which the upper 2/3 of the endometrial mucosa detach, the endometrial cells and inflammatory cells produce proteolytic lysosomial enzymes causing a tissue breakdown whit consequent tissue necrosis, disruption of microvasculature, migration of leukocytes and platelet/fibrin thrombosis in microvessels. On the other hand, during AUB, only the superficial layer (subsurface) detaches, diffusely (withdrawal bleeding) or focally (breakthrough bleeding). In the presence of the second evenience, CE and/or microerosions or vascular fragility due to structural abnormalities of microvessels could be frequent. However endometritis and microerosions may occur in otherwise normal endometrium, polyps, submucosal leiomyomata, atrophy and cancer (organic causes). In that condition, Gram negative bacteria, mycoplasma and other infective agents may evoke a Th-1 response with endotoxin, macrophages, IL-1, and TNF-alfa local secretion, determining a pro-inflammatory response, responsible of damage of the conceptus, implantation failure, spontaneous abortion and preterm delivery, when acting at endometrial level, and hypercontractility and pain when acting at myometrial level. The clinical diagnosis of CE is quite difficult. Physical signs are represented by uterine tenderness, spontaneous and at mobilization, leucorrhoea, cervical cyanosis, easy intracervical bleeding, but in most of cases is totally asymptomatic. Ultrasounds not specific signs are usually represented by endometrium out of phase, increased endometrial thickness and presence of endometrial fluid. In consideration of that, the role of hysteroscopy appears not only important but necessary. At CO2 hysteroscopy CE is Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 50 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 0 5 10 15 20 25 30 35 E. coli Strept. Staphyl. Enter. Faec. Chlam. Ureapl. Yeast % characterised by areas of red endometrium, flushed, with a white central point, localized or scattered out the cavity with the typical "strawberry aspect“described by Cravello. More recently for that purpose, Cicinelli proposed the fluid diagnostic minihysteroscopy which characterizes for being painless and safe, performable also in case of bleeding (continuous-flow), easy to perform (learning curve shorter than with gas hysteroscopy), smooter distention of the uterine cavity (less pain), low costs and floating of ingrowths, with possible detection of subtle lesions. In detail, while saline solution allows floating of ingrowths while CO2 causes flattening of ingrowths against the endometrial surface and, therefore, the diagnostic images obtained at fluid hysteroscopy may do not correspond to those described at traditional CO2 hysteroscopy. Cervical signs of CE in course of fluid mini- Hysteroscopy may be represented by hyperemia, easy bleeding, cervical polyps, micropolyps, adhesions and leucorrhoea. On the other hand, endometrial signs are endometrial edema in the follicular phase (out of phase endometrium), diffuse or focal hyperemia (periglandular), endometrial polyps, adhesions and micropolyps, often representing, however, subtle lesions of difficult identification. Endometrial micropolyps are described as an atypical aspect of endometrial surface characterized by the presence of very small (less that 1 mm of size) peduncolatous polyps showing a vascular axis. These subtle lesions may be sporadic or may cover most of the endometrial surface. According to Cicinelli, 93.7% cases of micropolyps showed CE at histology and endometrial micropolyps are detected in 53.6% of all cases of CE at histology. The presence of micropolyps shows a sensitivity of 54% and a specificity proxime to 99%, and the likelihood to have histological diagnosis of CE for women with micropolyps is very high (O.R. 124.2, C.I. 50.3 to 205.4). As regards the etiology, the prevalence as percentage of infectious agents detected at vaginal (blue bars), at endocervical (red bars) and at endometrial cultures (yellow bars) in women with signs of chronic endometritis at hysteroscopy, according to Cicinelli et al. is represented in the following figure. These studies have been prevalently oriented at the definition of CE in order to investigate its role in the blastocyst implantation process. The human reproduction represents, in fact, a classical paradox, according to which it is critical to the survival of the species but relatively inefficient. Maximal fecundity (the probability of conception during one menstrual cycle) is approximately only 30% (Zinaman et al., Fertil Steril 1996); only 50 to 60% of all conceptions advance beyond 20 weeks of gestation (Wilcox et al., N Engl J Med 1988); of the pregnancies that are lost, 75% represent a failure of implantation and are therefore not clinically recognized as pregnancies (Wilcox et al., N Engl J Med 1988); failed implantation is also a major limiting factor in assisted reproduction (Spandorfer & Rosenwaks, 1999); delivery rate per retrieval is 29.4%. The implantation process occurs 6-7 days after conception (fertilization) and, in primates, includes 3 stages: Apposition: initial adhesion of the blastocyst to the uterine wall. Microvilli on the apical surface of syncytiotrophoblasts interdigitate with microprotrusions from the apical surface of the uterine epithelium (pinopodes). Adhesion: characterized by increased physical interaction between the blastocyst and the uterine epithelium. Invasion: syncytiotrophoblasts penetrate the uterine epithelium. By then, the blastocyst is oriented with its embryonic pole toward the uterine epithelium. The uterine receptivity represents the state during the period of endometrial maturation when the blastocyst can become implanted and the optimal period for implantation ranges between days 20 to 24 of a regular 28-day menstrual cycle. Uterine receptivity features include: endometrial histologic changes, more vascular and edematous glands, enhanced secretory activity; pinopodes develop on the Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 51 luminal surface of the epithelium and myometrial activity changes. Successful implantation is the end result of complex molecular interactions between the hormonally primed uterus and a mature blastocyst and the failure to synchronize the component processes involved in these interactions may results in a failure of implantation. Implantation factors, apart from the hormonal influence by estradiol and progesterone, include peptide hormones, growth factors and cytokines acting with autocrine/paracrine activity, involved in the cross-talk between endometrium and blastocyst. According to these new acquisitions, leukemia inhibiting factor (LIF) could be considered as a new markers of endometrial receptivity. In detail its levels in both the luminal and glandular epithelium of the uterus rise dramatically in the midsecretory phase of the menstrual cycle and a diminished secretion of this factor is associated with recurrent pregnancy loss. Similarly, also adhesion molecules (integrins) and mucins are being considered valuable markers of endometrial receptivity. Mechanisms that enable the blastocyst to actively initiate implantation include: catecholestrogens, leukemia inhibiting factor, transforming growth factor, platelet-derived growth factor, insulin-like growth factor II, colony-stimulating factor 1, interleukin-1, interleukin-6, prostaglandin E2, plateletactivating factor and epidermal-growth-factor receptors and heparan sulfate proteoglycans able to interact with factor–like ligands. The finest understanding of the processes involved in inflammatory factors could be helpful to better manage the embryonic implantation failures in assisted reproduction and could pave the way to innovative diagnostic and therapeutic protocols which, in the next years, could be responsible of a pregnancy rate increase, in opposition to the “human reproduction paradox” quoted before.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of Necrotic Blastomere Removal On Vitrified Warmed 4-Cell Stage Mouse Embryos Development
249797
EN
Fathi
R
Eftekhari-Yazdi
P
Rezazadeh Valojerdi
M
Journal Article
2022
02
28
<strong>Introduction:</strong> The cryopreservation of embryos has become an integral part of ART. The aim of this study was evaluating the necrotic blastomere removal effect on vitrified –warmed 4-cell stage mouse embryos development.
<strong>Materials and Methods:</strong> The retrieved 2-cell mouse embryos were cultured in G1TM ver3 medium until the 4-cell stage, then the embryos were vitrified with pretreatment and vitrification solutions. After warming the embryos were transferred to G1TM ver3 medium, and divided into intact (control) and partially (25%, 50% and 75%) damaged groups. In the partially damaged groups, the necrotic blastomeres were removed after zona pllucida laser hatching and embryos of two groups (control and removal) after reached to 8-cell stage were cultured for additional two days into G2TM ver3 medium. Finally, the rate of blastocyst formation, number of total blastomeres, number of blastomeres in each part of the inner cell mass (ICM) and trophoectoderm (TE) and apoptotic cells were compared statistically using c 2 and ANOVA tests. <strong>Results: </strong>The blastocyst formation was increased in all partially damaged groups after removal of necrotic blastomeres. This increase was only significantly (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Structure Of Cartilage Generated By MMSCs In Vitro Somewhat Differs From That Of In Vivo Cartilage
249798
EN
Baghaban Eslaminejad
M.R
Taghiyar
L
Piryaei
A
Journal Article
2022
02
28
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Mature Chondrocyte Promotes Cartilage Differentiation Of MMSCs In Co-Culture Systems
249846
EN
Baghaban
Eslaminejad M.R
Taghiyar
L
Piryaei
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison Of Potential Proliferation And Growth Of Mouse Urothelial Cells Cultivated On Three Individual Matrixes Include Human Amniotic, Peritoneal And Omentum Membranes
249847
EN
Sharifi Aghdas
F
Hamzehi
N
Moghadasali
R
Ghaemimanesh
F
Baharvand
H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Alpha-Fetoprotein Promotes Growth Activity Of Three Bone Marrow Hematopoietic Stem Cell Subpopulations Ex Vivo
249848
EN
Rybakova
T.M
Fomychova
E.V
Dubeshko
S.Yu
Belyaev
N.N
Bogdanov
A.Yu
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Autologuse Serum Could Significantly Improve The Viability And Proliferation Index Of Rat Mesenchymal Stem In Vitro
249849
EN
Hamrahi
D
Baharvand
H
Arab Njafi
S.M
Baghban Eslami
Nejad M.R
Rouhi
L
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ultrastructural Features Of Apoptosis In Gametogenic Cells Exposed To Environmental And Chemical Factors
249850
EN
Nikzad
B
Soleimani Rad
J
Roshangar
L
Mohammadnejad
D
Aazami
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Selective Growth Of Epithelial Basal Cells From Human Prostate In A Three-Dimensional Organ Culture
249851
EN
Papini
S
Rosellini
A
Revoltella
RP
Campani
D
DeMatteis
A
Selli
C
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Differentiation Unrestricted Somatic Stem Cells (USSCs) Into Cartilage
249852
EN
Kaviani
S
Soleimani
M
Nejhad Dehbashi
F
Heidari
S
Atashi
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Effect Of Silk Proteins On Osteoblast Differentiation Of Rat Bone Marrow Stromal Cell Cultures
249853
EN
Morsali
F
Nikbakht
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Induction Of Arterial Calcification In The Vascular Smooth Muscle Cell Cultures By A Potent Calcium-Channel Blocker Drug
249854
EN
Morsali
F
Nikbakht
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comprehensive Effect Of Different Growth Factors For Differentiation Of Rat Bone Marrow Mesenchymal Stem Cells Into Hepatocyte-Like Cells
249856
EN
Kaviani
S
Sepehri
H
Soleimani
M
Nasiri
Z
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
A New Human Feeder Layer For Expand Embryonic Stem Cell In Vitro
249857
EN
Atashi
A
Heydari
S
Solimani
M
Bahmani
M
Kaveani
S
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Umblical Cord Blood- Derived Unrestricted Somatic Stem Cell, A New Human Feeder Layer For Expand Embryonic Stem Cell In Vitro
249858
EN
Dehbashi
F
Solimani
M
Heydari
S
Kaveani
S
Rezaei Taverani
M
Bahmani
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Application Of Proteomics To Identify Human Embryonic Stem Cell-Associated Proteins
249859
EN
Gurabi
H
Pakzad
M
Baharvand
H
Fathi
A
Hosseini Salekdeh
Gh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Nuclear And Total Proteome Analyses Of Monkey Embryonic Stem Cell Differentiation
249860
EN
Taei
A
Baharvand
H
Nasrabadi
D
Hosseini Salekdeh
Gh
Mollamohammadi
S
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
OCT-4, An Embryonic Stem Cell Marker, Is Gighly Expressed In Bladder Cancer
249873
EN
Bahrami
A.R
Ziaee
S.A.M
Atlasi
Y
Mowla
S.J
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Attachment And Spreading Kinetics Of Stem Cells Using Optical Waveguide Lightmode Spectroscopy
249875
EN
Ramsden
J.J
Horvath
R
McColl
J
Aref
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of 5-Azacytidine On Bone Marrow Stromal Cells Differentiation Into The Cardiomyocyte-Like Cells
249876
EN
Fathi
F
Harighi
M.J
Amini
A
Azadfar
N
Azadbakht
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cells In Ophthalmology
249877
EN
Sayyahmelli
S
Morsali
S.M
Alipanahi
R
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Developmental Congenital Ocular Tumors: A Rare Clinicopathologic Report Of Globe Xantoma In 8 Month Child
249878
EN
Sayyahmelli
S
Morsali
S.M
Alipanahi
R
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Meiotic Competence And DNA Damage Of Porcine Oocytes Within Follicles After Exposure To Elevated Temperature
249879
EN
Wongsrikeao
P
Agung
B
Barati
F
Otoi
T
Nagai
T
Taniguchi
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ultrastractural Effects Of Superovulatory Drugs On Human Glandular Endometrial Epithelium At LH+6
249880
EN
Mohammadnejad
D
Roshangar
L
Bazy
P
Soleimani
Rad J
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Risk Factors For IUD Failure: Results Of A Large Multicentre Case- Control Study
249881
EN
Maria
B
De La Rochebrochard
E
Almont
T
Thonneau
P
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of Temperature On The Outcome Of Intrauterine Insemination
249882
EN
Ghalambor Dezfouli
F
Saharkhiz
N
Nikbakht
R
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison Of Twin Pregnancy Outcome After Assisted Reproductive Technology With And Without Embryo Reduction
249883
EN
Mostajeran
F
Gharavi
H
Ahmadi
S.M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effect Of Prednisolone Therapy On Delivery Outcome In Women With The Past History Of Idiopathic Spontaneous Abortion
249884
EN
Niroomand
N
Alimadad Tafreshi
A
Moinfar
Z
Mirzaaghaee
F
Davari Tanha
F
Ghaseminejad
A
Akbari Asbagh
F
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Cesarean Scar Pregnancy Misdiagnosed As Gestation In Bicornate Uterus
249885
EN
Moinfar
Z
Davari Tanha
F
Mirzaaghaei
F
Khalilpour
Z
Niroomand
N
Samimi Sede
S
Mohamadpour
J
Khanmohamadi
F
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Predictive Value Of Basal Antaral Follicle On Intra Cytoplasmic Sperm Injection (ICSI) Outcome
249886
EN
Mehrafza
M
Mohammad Tabar
Z
Hoseeieni
A
Nikpuri
Z
Oudi
M
Asgharnia
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
An In Vitro Comparative Study Of Follicle Stimulating Hormone (FSH) And Activin A Effects On The Maturation Of Preantral Follicle-Enclosed Oocytes From Immature Syrian Mice
249887
EN
Javed
A
Zarchi
S
Rezaei Zarchi
S
Kalantar
S.M
Jamil
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Neonatal Birth Weight After Assisted Reproduction
249888
EN
Zhang
H
Steimann
S
De Geyter
M
De Geyter
C
Holzgreve
W
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Galectin-9: Spatial Expression And Subcellular Localization In The Human Endometrium
249889
EN
Kabir-Salmani
M
Azadbakht
M
Iwashita
M
Rezazadeh Valojerdi
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison Of The Accuracy Of Out-Patient Sonohystrography With Transvaginal Sonography For The Screening Of Causes Leading To Abnormal Uterine Bleeding
249890
EN
Ravanbakhsh
R
Mahmoodian
N
Alborzi
S
Dehbashi
S
Alborzi
M
Ghotbi
Sh
Parsanezhad
M.E
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Repeated Spontaneous Abortion (RSA): A Challenge To Find A Clue Within The Cytokine Network
249891
EN
Samsami
A
Naimi
S
Ghaderi
A
Amani
D
Zolghadri
J
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Health Of Children Born Using Assisted Reproductive Technology– Especially ICSI
249892
EN
Tabatabaei
V
Alizade Nouri
R
Rezvani Far
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Secret Of ART Children
249893
EN
Djalalinia
Sh
Peikari
N
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Various Sttitudes About ART Ethics
249894
EN
Djalalinia
Sh
Peikari
N
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Relationship Between Exercise And Quality Of Life In Menopausal Women
249895
EN
Anderson
D
Mirzaii Najmabadi
Kh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ethical Issues In Embryo Research
249896
EN
Peikari
N
Djalalinia
Sh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Bioethics In Maternity Research
249897
EN
Peikari
N
Djalalinia
Sh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Attitudes Toward Menopause, Quality Of Life, And Decreased Sleep Quality Among Middle-Aged Women: A Community Survey In An Island Of Taiwan
249898
EN
Fuh
J.L
Wang
P.H
Wang
S.J
Cheng
M.H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Affirmation Of Embryo Transfer At Blastocyst Stage In Assisted Reproductive System
249899
EN
Keshavarze
H
Bazzi
P
Zahiri
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Vitrification Of Human Oocyte Using Cryoloop
249900
EN
Ghalambour
F
Saki
G
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Inhibitory Effects Of Müllerian Inhibitory Substance (AMH) On FSH-Induced In Vitro And In Vivo Growth And Development Of Mouse Follicles
249901
EN
Javed
A
Rezaei-Zarchi
S
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Evaluation Of The Spindle Apparatus Of In Vitro Matured Mice Germinal Vesicle Oocytes After Vitrification
249902
EN
Sharifi
A
Nasiri
E
Abbasi
M
Mahmoudi
R
Sobhani
A
Dehghani
M
Roozbehi
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Effect Of Different Concentrations Of Bovine Preovulatory Follicular Fluid And Bovine Fetal Cord Serum On The Development Of Two-Cell Mouse Embryos In Vitro
249903
EN
Soleimani Rad
J
Tajik
P
Beheshti
R
Mohamadi Roshandeh
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
In Vitro Meiotic Maturation Of Mouse Oocytes: Role Of Nitric Oxide
249904
EN
Amidi
F
Abolhassani
F
Sato
E
Abbasi
M
Mohammadi
A
Mahmoudi
R
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Semen Changes After Extracorporeal Shockwave Lithotripsy For Distal Ureteral Stones
249905
EN
Sayed
M.A.B
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Assessment Of Cytotoxic Effects Of I.V. Injection Of Omnipaque 350 On Spermatogenesis In Mouse Model
249906
EN
Salouti
M
Mozdarani
H
Parvizi
Sh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Effect Of Alpha-Tocopherol Supplementation On Human Semen Quality And Sperm Parameters
249907
EN
Amanpour
S
Movahedin
M
Nazm Bojnordi
M
Ghasemi
H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Hormonal Abnormalities In Azoospermic Men In Kano, Northern Nigeria
249908
EN
Mohammed
A.Z
Uadia
P.O
Emokpae
M.A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Correlation Between Seminal Plasma Glutathione Peroxidase Enzyme Activity And Semen Parameters
249909
EN
Fazaeli
R
Eidi
M
Eidi
A
Shahmohammadi
P
Bahar
M
Dadgar
M
Pouyan
O
Saeidi
H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Is Seminal Plasma Fructose Concentration Associated With Determinants Of Semen Quality?
249910
EN
Pouyan
O
Eidi
M
Shahmohammadi
P
Bahar
K
Saeidi
H
Bahar
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Varicocele And Antisperm Antibody: Fact Or Fiction?
249911
EN
Rezazade
M
Mehrsai
A
Djaladat
H
Pourmand
G.R
Djaladat
Y
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
In Vitro Effect Of Clomiphene Citrate On Motility And Vitality Of Sperm In Mouse
249912
EN
Khazaei
M
Ghorbani
R
Bakhtiari
M
Khazaei
MR
Purmotaabbed
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Effects Of Nitric Oxide Synthase Inhibitor (L-NAME) On Epididymal Sperm Count And Morphology In Varicocelized Rat
249913
EN
Abulhassani
F
Abbasi
M
Bahmanzadeh
M
Ejtemaiimehr
SH
Takzaree
N
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Umbilical Cord-Derived Cell Products For Treatment Of Congenital Heart Disease
249914
EN
Stamm
Ch
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Autologous Cultivated Limbal Stem Cell Transplantation In Cases With Unilateral Limbal Stem Cell Deficiency
249916
EN
Javadi
M.A
Mirbaei
F
Reaei Kanavi
M
Ebrahimi
M
Baharvan
H
Baradaran Rafiei
A
Einollahi
B
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-117: Functional Genomics Of Stem Cell Therapy
249917
EN
Sanati
M.H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
DNA Decays And Their Repair In The Oocyte
249918
EN
Menezo
Y
Benkhalifa
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Embryo Methabolism And Culture Media: Impact On Apoptosis And Imprinting
249919
EN
Menezo
Y
Benkhalifa
M
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-88:Embryology And 3d Sonoembryology
249920
EN
Kurjak
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
3D And 4D Sonography Inthe Assessment Of Infertile Women
249921
EN
Kurjak
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-85:GnRH Antagonist: The NewStandard For COS Protocols
249922
EN
Esmat
Kh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-84:Entering New Era InReproductive Medicine
249923
EN
Esmat
Kh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-83:Optimization Of OvarianStimulation For ChronicAnovulation
249924
EN
Hugues
J.N
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Recombinant FSH Filled By Mass: A Scientific Step For Clinical Improvement
249925
EN
Hugues
J.N
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-80:Modern Etiopathogeneticand Diagnostic Criteria For PCOS
249926
EN
Cianci
A
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Reconciling Donor AndRecipient In Oocyte DonationProgrammes
249927
EN
Pennings
G
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
An Ethical Framework Forthe Use Of Humanoocyte For StemCell Research
249928
EN
Pennings
G
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Implication Of Explanatory Model Of Iranian Women Sexualityin Development Of Sexual EducationProgram
249929
EN
Merghati Khoei
E
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Cloning And HumanDignity: Catholic And IslamicPerspectives
249930
EN
Eslami
S.H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
PGD Of Hereditary Cancers:Ethical Aspects
249931
EN
De Wert
G
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Future (R)Evolution OfPGD/HLA- Testing; Ethical Reflections
249932
EN
De Wert
G
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stress And Infertility AndUsing Stress ManagementTechniques
249933
EN
Alizadegan
Sh
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ethical Aspects Of Regulationof Assisted Reproduction In ArabSpeaking World
249934
EN
Al-Inany
H
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Blastocyst Culture AndTransfer-What Are The Essentials Tobe Successful
249935
EN
Wiemer
K
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
ART Lab Quality Control
249936
EN
Wiemer
K
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
A Unique Human Blood- Derived Cell Population Displays Embryonic Markers And High Potential For Producing Insulin
249937
EN
Lazzarini
P
Huang
Z
Zhao
Y
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Microgravity Potentiates Stem Cell Proliferation While Sustaining The Capability Of Differentiation
249938
EN
Ide
T
Kataoka
K
Asashima
M
Yamaoka
K
Wu
S.L
Yoshimoto
R
Umeda
C
Kawahara
Y
Tahara
H
Kajiume
T
Rui
Y
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
NPC1 Gene Deficiency Leads To Lack Of Neural Stem Cell Self-Renewal And Abnormal Differentiation Through Activation Of P38 Mitogen-Activated Protein Kinase Signaling
249939
EN
Yang
S.R
Kim
S.J
Byun
K.H
Hutchinson
B
Lee
B.H
Michikawa
M
Lee
Y.S
Kang
K.S
Journal Article
2022
03
02
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Developmental Plasticity Of Luteinizing Granulosa Cells Cultured Over Prolonged Time Periods In Culture Medium Supplemented With The Leukemia-Inhibiting Factor
249943
EN
Tomaszczuk
K
Zhang
H
Martin
I
De Geyter
M
Gao
S
Scherberich
A
De Geyter
Ch
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Pharmacoproteomics: Strategies For Identifying New Diagnostic And Prognostic Markers For Colorectal Cancer
249944
EN
Moritz
R.L
Ahn
S.M
Bernhard
O.K
Kapp
E
Goode
R.J.A
Lim
J
Greening
D
Ji
H
Simpson
R.J
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cells In Reproductive Medicine
249945
EN
Sim?n
C
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Genomics Of Endometrial Receptivity
249946
EN
Sim?n
C
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Basic Fibroblast Growth Factor Controls Migration In Human Mesenchymal Stem Cells
249947
EN
Klausmann
U
Brixius
K
Klinz
F.J
Ulrichs
Ch
Mehlhorn
U
Desai
B
Arnhold
S
Blocha
W
Addicks
K
Staib
P
Schwinger
R.H.G
Schmidt
A
Ladage
D
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Review Of The Methodologies For Differentiation Of Embryonic Stem Cells Into The Distal Airway Epithelium
249948
EN
Samadikuchaksaraei
A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Alpha-Fetoprotein Action On Clonogenic Activity, Self- Renewal And Differentiation Of Three Bone Marrow Hematopoietic Stem Cell Subpopulations Ex Vivo
249949
EN
Rybakova
T.M
Fomychova
E.V
Dubeshko
S.Yu
Belyaev
N.N
Bogdanov
A.Yu
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Enriched NCAM Positive Cells Form Functional Dopaminergic Neurons In The Rat Model Of Parkinson´S Disease
249950
EN
Ravindran
G
Rao
H.S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Primate Embryonic Stem Cells Create Their Own Niche While Differentiating In Three-Dimensional Culture Systems
249951
EN
Franceschini
V
Michelini
M
Revoltella
R.P
Sihui Chen
S
Ciani
F
Rosellini
A
Papini
S
Margolis
L
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Multilineage Differentiation Of Rhesus Monkey Embryonic Stem Cells In A Three-Dimensional Culture System
249952
EN
Revoltella
R.P
Chen
S.S
Zimmerberg
J
Margolis
L
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
RNA-Mediated Induction Of Hereditary Epigenetic Modification
249953
EN
Rassoulzadegan
M
Grandjean
V
Ghanbarian
H
Wagner
K
Wagner
N
Gounon
P
Cuzin
F
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cells Biology: PU.1 (Sfpi1), A Pleiotropic Regulator Expressed From The First Embryonic Stages With A Critical Function In Germinal Progenitors
249954
EN
Rassoulzadegan
M
Olive
V
Wagner
N
Chan
S
Kastner
P
Vannetti
Ch
Cuzin
F
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Induction Of Mixed Chimerism By Embryonic Stem Cells In Mice: Do Infused ES Cells Truly Differentiate?
249955
EN
Zavazava
N
Scoville
H
Bonde
S
Pedram
M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Generation Of Feeder-Free And LIF-Independent Bovine ES Cell Lines
249956
EN
Pashai Asl
M
Richings
N.M
Holland
M.K
Verma
P.K
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
BIO, GSK3 Specific Inhibitor, Promote Dopaminergic Differentiation Of Unrestricted Somatic Stem Cell
249957
EN
Mahmoodinia
M
Zeynali
B
Soleimani
M
Kaviani
M
Tafreshi
A.P
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Successful Stem Cell Therapy Using Umbilical Cord Blood-Derived Multipotent Stem Cells For Buerger’s Disease And Ischemic Limb Disease Animal Model
249958
EN
Kim
S.W
Han
H
Chae
G.T
Lee
S.H
Bo
S
Yoon
J.H
Lee
Y.S
Lee
K.S
Park
H.K
Kang
K.S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Generation And Phenotypic Analysis Of Klf13-/- Mice
249959
EN
Kemp
P
Metcalfe
J
Grace
A
Keramatipour
M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Differentiation Of Human Bone Marrow Derived Mesenchymal Stem Cell (HBMMSCs) To Hepatocyte In PCL Scaffold
249960
EN
Kazemnejad
S
Allameh
A
Soleimani
M
Gharebageian
A
Amirizadeh
N
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Mesenchymal (Skeletal) Stem Cells: Basic Biology And Clinical Applications For Bone Tissue Regeneration
249961
EN
Kassem
M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Differentiation Of Human Embryonic Cells Into Osteoblastic Cells
249962
EN
Kassem
M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Mesothelial Stem/ Progenitor Cells Of The Peritoneum Clonal Characterization, Isolation And Differentiation
249963
EN
Foroutan
T
Moallem
S.A
Jahangiri
L
Nazemia
F
Hosseini
A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
In Vitro Reprogramming Of Fibroblasts Into A Pluripotent ES Cell-Like State
249964
EN
Foreman
R
Meissner
A
Wernig
M
Jaenisch
R
Brambrink
T
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cells, Pluripotency And Epigenetic Reprogramming
249966
EN
Jaenisch
R
Boyer
L
Wernig
M
Meissner
A
Lee
T
Medeiros
L
Zeitlinger
J
Brambrink
T
Lander
T
Mikkelsen
T
Guenther
M
Levine
S.S
Young
R
Bernstein
B
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
In Vivo Neurogenesis Potentials Of Adult Mesenchymal Stem Cells
249967
EN
Izadpanah
R
Kriedt
Ch
Hood
J
Alvarez
X
Bunnell
B.A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Embryonic Stem Cells, Actual Applications And Challenge
249968
EN
Inzunza
J
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Differentiation Of Embryonic Stem Cell Derived Neurons To Cholinergic Neurons In Vitro; And In Vivo After Transplantation In The Rat Model Of Alzheimer’s Disease
249969
EN
Homayouni Moghadam
F
Baharvand
H
Nasr Isfahani
M.H
Alaie
H
Karbalaie
K
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Assessment Of The Cationic Colloidal Silica Method Of Plasma Membrane Enrichment For Use In Proteomic-Based Discovery Of Cell Surface Biomarkers
249970
EN
Goode
R.J.A
Ji
H
Simpson
R.J
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Exploiting Proteomics For Stem Cell Research
249971
EN
Hosseini Salekdeh
Gh
Baharvand
H
Fathi
A
Nasrabadi
D
Shekari
F
Momeni Moghaddam
M
Farokhi
A
Totonchi
M
Shahhosseini
M
Taei
A
Pakzad
M
Mollamohammadi
S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Polyamines Modulate Nitric Oxide Production And Cox-2 Gene Expression In Response To Mechanical Loading In Human Adipose Tissue- Derived Mesenchymal Stem Cells
249972
EN
Tjabringa
G.S
Vezeridis
P.S
Zandieh Doulabi
B
Helder
M.N
Wuisman
P.I.J.M
Klein-Nulend
J
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Microanatomical Study Of Effects Of Mesenchymal Stem Cells On Striatal Lesion By Quinolinic Acid
249974
EN
Edalatmanesh
M.A
Bahrami
A.R
Behnam Rasuli
M
Moghadam Matin
M
Moghimi
A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Spermatogonial Stem Cells: Effects Of Shorttime Exposure To High Temperature
249975
EN
Setchell
B.P
De Rooij
D.G
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Culture Of Spermatogonial Stem Cells
249976
EN
Mizrak
C.S
Aponte
P
De Rooij
D.G
Van Pelt
A.M.M
Ardekani
H.S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Transplantation Of Undifferentiated Mesenchymal Stem Cell In Distraction Osteogenesis In A Canine Model: A Histological And Histomorphometrical Study
249977
EN
Dehghan
M.M
Baghaban Eslaminejad
M
Motallebizadeh
N
Ashrafi Halan
J
Soroori
S
Kazemi Mehrjerdi
H
Ezadi
S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Role Of Notch Signaling In Normal And Neoplastic Hematopoietic Stem Cells, And Clinical Application Of Notch Signal Modifiers
249978
EN
Suzuki
T
Lee
S.Y
Saito
T
Nakagawa
M
Kumano
K
Chiba
S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Is There A Place For Peripheral Blood Hematopoietic Stem Cell Transplant In Solid Tumors?
249979
EN
Brusamolino
E
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Peripheral Blood Hematopoietic Stem Cell Transplant In Malignant Lymphoma Biology, Clinical Indications And Results
249980
EN
Brusamolino
E
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cells: From Bench To Bedside
249981
EN
Bongso
A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Embryonic Stem Cells: The Mother Of All Cells
249982
EN
Bongso
A
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Transcriptional Profiling Of Mammary Gland Side Population Cells Reveals New Roles For This Putative Mammary Gland Stem Cell Sub-Population
249983
EN
Rosen
J.M
Behbod
F
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Keratolimbal Allograft Surgery
249984
EN
Baradaran-Rafi
A
Abdolahi
A
Einollahi
B
Zare
M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Generate Retinal Cells From Retinal Stem Cells To Repair The Retina
249985
EN
Arsenijevic
Y
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Pluripotent Stem Cell Epigenetics During Development And Cancer
249986
EN
Silvan
U
DiezTorre
A
Andollo
N
Boyano
M.D
Arechaga
J.M
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Cancer Stem Cell
249987
EN
Alimoghaddam
K
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
High Resolution Mapping Of Ribosomal DNA In Early Mouse Embryos By Fluorescence In Situ Hybridization
249988
EN
Romanova
L
Korobova
F
Noniashvilli
E
Dyban
A
Zatsepina
O
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Murine Dnali1 Gene Encodes A Flagellar Protein That Interacts With The Cytoplasmic Dynein Heavy Chain 1
249989
EN
Neesen
J
Doerwald
N
Geisler
S
Hupe
M
Breckle
R
Rashid
S
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Preimplantation Genetic Screening (PGS)
249990
EN
Platteu
P
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Recent Concern On Genetic Of Infertility
249992
EN
Kalantar
SM
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Application Of Single-Needle Blastomere Biopsy In Human Preimplantation Genetic Diagnosis
249993
EN
Hrlund-Richter
I
Nordenskj?ld
M
Jones
B
Blennow
E
Hillensj?
T
Sj?blom
P
Rosenlund
B
Fridstr?m
M
Iwarsson
E
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Novel Mutations And (TG) M (T) N Polymorphism In Iranian Males With Congenital Bilateral Absence Of The Vas Deferens
249994
EN
Gourabi
H
Sedighi
M.A
Vosough
A
Radpour
R
Journal Article
2022
03
03
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Genetic Aspects Of Infertility And Its Treatment
250004
EN
Geraedts
J
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Regeneration Potency In The Animal Kingdom
250005
EN
Geraedts
J
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Poly (ADP-Ribose) Polymerase-2 Contributes To The Fidelity Of Male Meiosis I And Spermiogenesis
250006
EN
Dantzer
F
Mark
M
Quenet
D
Scherthan
H
Huber
A
Liebe
B
Monaco
L
Chicheportiche
A
Sassone Corsi
P
De Murcia
G
Me Nissier-de Murcia
J
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Chromosome Polymorphism In Infertile And Sub-Fertile Patients: A Pilot Study
250007
EN
Saremi
A
Shafaghati
Y
Ghasemi Firouzabadi
S
Bagheizadeh
I
Najmabadi
H
Kahrizi
K
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Assisted Reproductive Technology And Pre-Implantation Genetic Diagnosis For Genetic Disease-Free Children
250008
EN
Abbas
S
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Ovarian Interstitial YAGLaser: An Efftive New Method To Manage Anovulation In Women With Polycystic Ovary Syndrome
250009
EN
Zhu
W.J
Li
X
Chen
X
Lin
Z
Zhang
L
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Clinical Study Of Different Form Of Hysteroscopic Surgery For Endometrial Polyps
250010
EN
Yan
D
Gu-Qun
Sh
Jun-Qi
MA
Xing-Zi
AI
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Human Embryonic Implantation: Mechanisms And Consequences
250011
EN
Taylor
R.N
Journal Article
2022
03
05
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-100:Endometrial Angiogenesis:Physiology And Pathology
250012
EN
Taylor
R.N
Journal Article
2022
03
05
The functionalis layer of human endometrium is a highly dynamic tissue, undergoing dramatic cyclical remodeling throughout a woman’s reproductive life. The uterine lining typically grows from a thickness of 0.5 mm in the postmenstrual phase to >5 mm in the late proliferative and secretory phases of the endometrial cycle. The robust proliferation of endometrial epithelial and stromal cells is accompanied pari passu by extensive vascular and capillary growth, which provides the oxygen and nutrients needed to support the cyclic tissue expansion. Excessive angiogenesis is associated with endometrial polyps and neoplasia, whereas reduced angiogenesis appears to lead to defects in endometrial receptivity, embryonic loss and pregnancy complications associated with failed placentation. Our laboratory has been interested in the endocrine factors that mediate capillary proliferation in the human uterus and we have focused predominantly of the role of vascular endothelial growth factor (VEGF). This gene product is the most potent mitogen for capillary endothelial cells and it is highly expressed in endometrial epithelial and stromal cells. We have determined that the human VEGF gene is under direct transcriptional control by estrogen receptor complexes liganded to 17-beta estradiol (E2) or synthetic estrogens. We identified a unique variant estrogen responsive element in the human VEGF gene promoter that mediates the stimulatory effects of estrogens on VEGF production in endometrial cells. Progesterone (P4) also imposes proangiogenic effects on endometrial cells via the upregulation of VEGF gene and protein expression, although its mechanism of action is more mysterious. Unbalanced progestins may contribute to abnormal uterine bleeding associated with contraceptives. The combination of E2 and P4, as observed in the midluteal window of implantation appears to optimize the vasculature for embryonic receptivity. By contrast, anti-angiogeneic therapeutics may be useful in conditions associated with excessive endometrial vascular growth, such as polyps, endometriosis or contraceptive breakthrough bleeding.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-99:Inflammatory Mediators OfEndometriosis Pathogenesis
250013
EN
Taylor
R.N
Journal Article
2022
03
05
Endometriosis affects about 70 million women world-wide and accounts for 1-2 billion US dollars in medical and surgical health expenses annually. A variety of theories has been promulgated regarding the etiology of endometriosis, yet the precise pathogenic mechanisms responsible for its associated pelvic pain and infertility remain unknown. The establishment of endometriosis lesions in extrauterine locations (eg, peritoneum, ovary or rectovaginal septum) requires the invasion and neovascularization of endometrial stromal and gland cells within the extracellular matrix of the ectopic foci. Recruitment of innate immune cells into endometriotic lesions and the peritoneal cavity is a key pathophysiological feature of the syndrome. Neutrophils and macrophages are attracted from the circulation via chemokines, including ENA-78, eotaxin, IL-8, MCP-1 and RANTES. This presentation will focus on the latter, a C-C or beta-chemokine with chemoattractant properties for monocytes, T cells and eosinophils that has been extensively characterized in our laboratory. Concentrations of RANTES are elevated in the peritoneal fluid of women with endometriosis and correlate with disease stage. Stromal cells in ectopic implants appear to be the primary cellular source of RANTES. Analyses of the human RANTES gene promoter in endometrial and endometriotic cells indicate that proinflammatory cytokines (eg, IL-1-beta and TNF-alpha) regulate RANTES expression via the activation of the transcription factor NF-kappaB. Estrogens and dioxins can increase RANTES production and appear to exacerbate endometriosis. Agents that inhibit NF-kappaB signaling reduce RANTES mRNA and protein production. Two general classes include certain nonsteroidal anti-inflammatory drugs (NSAIDs) and thiazolidinediones (TZDs). These pharmaceuticals have been tested using in vitro cultures of human endometriosis cells and also in mouse and baboon models of endometriosis. These compounds show promise in the reduction of lesion volume. The findings of these preclinical studies promise that new opportunities for drug discovery exist and that refinement of treatment choices for endometriosis will emerge.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-98:Two Novel Techniques ToDetect Follicles In Human OvarianCortical Tissue
250015
EN
Soleimani
R
De Vos
W
Van Oostveldt
P
Lierman
S
Van Den Broecke
R
De Sutter
P
Dhont
M
Van Der
ElstJ
Journal Article
2022
03
05
<strong>Background:</strong> Ovarian tissue cryopreservation and transplantation are becoming increasingly important issues for preserving female fertility as shown by recent successes in restoring ovarian activity and even fertility. Primordial follicle content before transplantation is a key issue for success. We investigated two novel methods to detect primordial follicles in human ovarian cortical tissue strips.
<strong>Materials and Methods: </strong>The first method used the fluorescent mitochondrial stain rhodamine 123 in combination with laser scanning confocal microscopy (LSCM). The second used a simple stereomicroscopic method with glass-bottomed dishes for detecting primordial follicles in cortical ovarian tissue strips. Potential toxic effects of R123 and of the exposure to confocal laser were investigated in a mouse ovarian allograft model.
<strong>Results:</strong> Follicles were visible as white spots in thin cortical strips using LSCM in single and fast scanning at low magnification, allowing a fair estimation of the number of primordial follicles present. Using the second method, ovarian follicles were also visible using glassbottom dishes under the stereomicroscope, although tissue thickness and density were limiting factors of its success.
<strong>Discussion:</strong> Follicles can be visualized in human cortical ovarian strips with R123 in combination with LSCM. Stereomicroscopy using glass-bottomed dishes and transmitted illumination is a simple alternative method and has the advantage of allowing further safe clinical use of the analysed tissue.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-97:Challenges Of OvarianFollicle Culture
250016
EN
Smitz
J
Journal Article
2022
03
05
Follicle culture systems are interesting models to learn about animal and human reproductive physiology. The choice of the culture technique is based upon: the size of the ovary (the species), the developmental stage of the follicle of interest, and the duration of folliculogenesis. The success of a culture system is judged by its ability to produce developmentally competent oocyte. Until today only the mouse follicle culture is a successful system. This follicle culture model will be described. Problems associated with the large size of the ovary (human and domestic species) can be solved by culturing thin slices of tissue. Unfortunately, adult ovarian tissue is not the ideal material to attempt to develop a human follicle culture system as their follicle density is low. The stromal tissue in the ovaries of larger mammals is fibrous and dense, and therefore does not facilitate the development of a successful system for isolation and culture of follicles. More research still needs to be accomplished on small follicles. The major requirements for normal growth of follicles at the earliest stages are still unknown and must be determined if developmentally competent oocyte are to be finally obtained. Fetal ovaries are useful experimental material because of their high follicle density and the softness of the tissue. Healthy T layered follicles in culture grown from primordial fetal follicles were morphologically comparable to those grown in vivo in the vast majority of cases. However, cellular development under culture conditions might be more susceptible to genetic changes. Long-term culture could result in inappropriate cellular differentiation, genetic modification and formation of tumour cells.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-96:Differences In Endocrine AndParacrine Milieu Induced By HPhMG(MENOPUR) Compared TorFSH (GONAL-F) Influencing LifeBirth Rate
250017
EN
Smitz
J
Journal Article
2022
03
05
It has been demonstrated in several models that the LH activity brings a dimension of quality in the cohort of follicles recruited by FSH. Evidence is accumulating that a supplementation of LH-activity independent of endogenous LH levels might be beneficial for pregnancy outcome. Recent data suggest differences in efficacy of treating women with follicle-stimulating hormone (FSH) alone and gonadotrophins containing LH-activity. LH plays a dual role in folliculogenesis. It promotes follicular development and maturation through the production of androgen and growth factors in theca and granulosa cells. Additionally, LH appears to be involved in the negative selection of smaller follicles within the first few days of the stimulation–probably via a shift in the androgen/estrogen balance. Unlike recombinant follicle-stimulating hormone (rFSH), highly purified human menopausal gonadotrophin (MENOPUR) contains both FSH and human chorionic gonadotrophin (hCG), a source of long-acting LH-activity. Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 57 The Menotrophin versus Recombinant FSH in in vitro fertilisation Trial (MERiT®) showed a trend towards a reduced number of oocytes at retrieval, which nonetheless, developed into a significantly higher number of top quality embryos per oocyte retrieved with MENOPUR versus rFSH. The hCG-driven LH-activity in MENOPUR happens to be the key differentiator driving the selection towards better quality follicles for in vitro fertilisation cycles. The different gonadotrophins lead to an endocrine profile with a major impact on the endometrium. MERiT found that progesterone levels were significantly lower on the days of hCG and oocyte retrieval with MENOPUR compared with rFSH and these lower progesterone levels were associated with higher implantation and ongoing pregnancy rates. Further analysis of cumulus-corona cells in patients treated with MENOPUR or GONAL-F showed a significant difference in gene expression on the day of oocyte retrieval in approximately 500 genes. Some of the genes changed by Menopur are known quality genes expressed in cumulus after stimulation. This data (unpublished from the authors' laboratory) might lead to a better understanding of cumulus-corona cell function in relation to oocyte quality and is an interesting subject of research. Together, these data suggest that the hCGdriven LH-activity present in MENOPUR brings a significant change in the close environment of the oocyte and in the hormonal balance that might influence endometrial receptivity, compared with rFSH.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-95:A New Proposed Techniquefor Chorionic Villus Samling
250018
EN
Journal Article
2022
03
05
<strong>Introduction:</strong> One of the techniques used for prenatal diagnosis is CVS. In contrast to amniocentesis (AC) carried out between 16 to Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 56 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 18 weeks gestation (second trimester), CVS can be done before 12 weeks of gestation (first trimester). Currently CVS can be done using both trans-abdominal (TA) and Transcervical (TC) techniques. Both methods are done between 9th and 11th weeks gestation under the ultrasound guidance. There is however controversy as to which technique is better, although TC is preferred when placenta is in a posterior position. Compared to amniocentesis, there is a higher miscarriage rate following CVS. Brambati et al (1991), in a randomized study showed that miscarriage rate in CVS done by TA was 16.5% compared to 15.5% in TC method. Various studies have demonstrated that CVS done by TC encounters more bleeding and sampling might need repeating. Since the current CVS methods inherit some problems such as bleeding and miscarriage, there is therefore a need for a safer method. In this study a new technique for CVS using transvaginal myometrium (TVM) has been introduced.
<strong>Materials and Methods:</strong> we investigated 362 patients referred to Sarem hospital from different genetic centers in Tehran. CVS was carried out on the patients using either TC or TVM randomly. All the patients were monitored by ultrasound and those with demised fetus, unsuitable anatomy, occurrence of bleeding in the previous week, twin pregnancies, and cervical fibroma were excluded from the study. The patients were aware of the CVS technique and its disadvantages. For TVM procedure the patients were placed in a lithotomic position. The vagina was cleaned using sterile swab and physiological saline. The position of fetus and placenta was monitored using ultrasound with vaginal probe. After positioning placenta in the movement path of a needle placed in a special guide and over the vaginal probe, the needle was directly inserted in the placenta through adjacent furuncles and myometrium. A 20 ml syringe containing 5cc culture medium and heparin was attached to the needle. Using negative pressure the villus was aspirated inside the syringe and with the same pressure, the needle was removed from the placenta and myometrium. Using dissection microscope the villus was checked and then the patient was transferred to the recovery room. CVS using TC was done using standard techniques.
<strong>Results:</strong> For three patients CVS was repeated on the same day. The average age of the patients was 28.9± 7.4. years. CVS was carried out at 10.8± 1.5 weeks. Following CVS with TC method, there was one miscarriage, one oligohydramnio, and one case with bleeding. In contrast TVM technique had no adverse effect.
<strong>Conclusions: </strong>CVS using TVM technique seems to be a safe technique and a better alternative to TC. However for a more conclusive result, further studies using a bigger sample size is warranted.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-94:A Study Of OvarianAutitransplantation WithoutVascular A Pedicle In Rats
250019
EN
Risvanli
A
Timurkan
H
Akpolat
N
Gulacti
I
Ulakoglu
E
Journal Article
2022
03
05
<strong>Objective: </strong>The study aimed to determine effects of FSH applications on follicle survival, development and hormone output and antigenicity of rat ovarian tissue autografts placed at subcutaneous or subperitoneal sites.
<strong>Materials and Methods:</strong> A total of sixteen female rats were used in the study. The animals were divided into three groups. Ovaries were dissected and then transplanted under the peritoneum in the first group animals (n = 5) or under the skin in the second group animals (n = 6). And the animals in the third group (n = 5) were sham operated. Following operations, intramuscular injection of 8 IU of rhFSH were made daily to the animals in first and second groups from the first day of operation through thirty days. Vaginal irrigation samples were prepared daily from the animals for 30 days. The concentrations of serum estradiol and antiovarian antibodies in the blood were determined using ELISA on the last day of vaginal irrigations. Histopathological examination of the ovaries that were transplanted was made.
<strong>Results: </strong>Results showed that cyclic variations were noticed in the samples of vaginal irrigation by day 30 in the animals of first and second groups. However, no significant differences were seen between groups. The concentration of blood serum estradiol was higher in the animals of first group. Decrease in numbers of primary follicles were found in the animals of second group and lesser corpus luteum were found in the animals of control group on the histopathological examinations of transplanted ovaries. All rats in the first and second groups were defined as seropositive for antiovarian antibodies. When the OD values were compared between first and second groups, it was identified that the OD values of rats in the first group was higher than it was seen in the second group.
<strong>Conclusion: </strong>The ovarian transplantation without vascular pedicle in rats is characterized by follicular hyperplasia endocrinologically functional. Being seropositive of all rats in first and second groups in terms of antiovarian antibodies is an indicator to these antibodies does not affect the functions of transplanted ovaries. It is believed that the highness of OD values in the group which is transplanted beneath the peritoneum is based on the highness of estradiol concentrations in these animals.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-93:In Vitro Maturation OfOocytes
250020
EN
Ramezanzadeh
F
Journal Article
2022
03
05
In vitro maturation (IVM) of human oocytes is technically mastered for a decade. The patients groups included in the studies have been PCOS, male factor infertility and other causes of infertility. Both women with normal ovaries and PCO have been included. This method was developed in which pre-ovulatory immature oocytes were aspirated without gonadotrophin stimulation or low hormonal stimulation and matured in an incubator. To increase the pool of potentially competent oocytes and to make the oocyte pick-up easier, FSH priming has been used in some selected patients, primarily in women with PCOS. The results from randomized studies have shown conflicting results, some reporting better pregnancy rates with FSH priming and some showing no beneficial effect. In vivo, gonadotropin plays an important role in the regulation of oocyte growth and maturation to trigger the meiosis resumption and nuclear maturation of oocytes in vivo. Recent studies reported that hCG priming before oocyte retrieval not only could enhance the oocyte maturation rate but also could improve the developmental potential of oocytes in vitro and increase clinical pregnancy rates. hCG priming also may aggravate nonsynchronization of oocyte nuclear and cytoplasmic maturation and which is considered to be a main reason of the poor development competence of IVM oocytes. So the effects of human chorionic gonadotropin (hCG) on oocyte maturation and development in vitro are still controversial. Of course use of hCG before immature oocyte retrieval in unstimulated ovaries make the maturation process faster and the number of MII oocytes will be higher, but there is no statistically demonstrable difference in the on-going pregnancy rate, endometrial parameters such as endometrial thickness, endometrial pattern ,or endometrial and uterine blood flow. In some centers hCG is generally added to culture medium when mammalian and human immature oocytes are cultured in vitro but recent studies indicated that the addition of hCG to in vitro culture medium did not improve the maturation rate or development potential of immature oocytes. For the IVM and development of immature oocytes from women with PCOS, hCG appears to be unnecessary. In fact in IVM method oocytes are typically retrieved from cycles which are nearly similar to natural cycles, then matured in vitro and fertilized by intracytoplasmic sperm injection. But reported implantation and pregnancy rates of this method are much lower than that achieved by IVF after controlled ovarian hyperstimulation (COH). It can be expected that up to a certain extent, the in-vitro environment could be improved in a way to better support the final maturation stages of the oocyte. Nevertheless, the stages of oocyte development before oocyte-cumulus retrieval are equally important: it is during the earliest stages of follicle development that RNA and protein stores are foreseen for later development of the oocytes until implantation. Therefore the destiny of part of the oocytes is already determined at their retrieval. It is hypothesized that cumulus cells could reflect oocyte health and bear the markers for developmental competence. Rapid measurements for these markers could be of help to the embryologist to select the developmentally competent oocytes to include in culture. Recent investigations have focused on the structural characteristics of chromatin and meiotic spindle of IVM oocytes, but little research was done concerning their chromosomal abnormalities, so their safe clinical use is still questionable. Recent Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 55 studies show the aneuploidy rate of IVM GVoocytes is comparable to the aneuploidy rate of in vivo matured oocytes and first polar bodies, regardless of the length of maturation period. Among young women affected by malignancy, the window of opportunity for fertility preservation is often limited by inadequate time to undergo ovarian stimulation or contraindication to hormonal stimulation in certain type of cancer. For these patients immature oocyte retrieval and IVM were performed prior to commencing cancer treatment. This is a new indication of IVM and embryo cryopreservation. Therefore immature oocyte retrieval followed by in vitro maturation (IVM) opens a new horizon for modern assisted reproductive technologies (ART), and recent studies in IVM make it a feasible alternative to in vitro fertilization.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
P-92:Clinical Implications Of HCGfor Ovarian Stimulation
250021
EN
Platteau
P
Journal Article
2022
03
05
The gonadotrophin preparations commonly used in assisted reproductive technology (ART) procedures contain similar folliclestimulating hormone (FSH) concentrations but differ extensively in their luteinizing hormone (LH)-activity1. MENOPUR (highly purified human menotrophin) has the highest concentration of human chorionic gonadotrophin (hCG) – a source of long acting LH-activity – of all commercially available gonadotrophin preparations. The hCG-driven LH-activity found in MENOPUR has been demonstrated to positively influence ART outcomes at the level of the embryo and endometrium. The European and Israeli Study Group (EISG) investigation and, more recently, the Menotrophin versus Recombinant FSH in vitro fertilization Trial (MERiT) established the impact of hCG-driven LH-activity in in vitro fertilization (IVF)2,3. In patients undergoing IVF, MENOPUR has shown equivalent safety profiles and a significantly higher live birth rate compared with recombinant FSH (rFSH)4,5. MERiT showed that the hCG-driven LHactivity in MENOPUR resulted in different hormone profiles compared with rFSH6. Progesterone levels at the end of stimulation Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 54 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 and on the day of oocyte retrieval were significantly lower in the MENOPUR patients compared with the rFSH group6. This has a probable impact on the quality of the endometrium and subsequently, embryo implantation. Additionally, it was shown that hCG levels on day 6 were highly predictive for ongoing pregnancy after IVF7. This suggests that hCG should be given from the beginning of stimulation, and possibly at higher doses, in patients who have reduced hCG levels on day 6 in order to increase the likelihood of pregnancy.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-91:Hysteroscopic Metroplasty Ofthe Complete Uterine Septum,Duplicate Cervix, And VaginalSeptum
250022
EN
Parsanezhad
M.E
Alborzi
S
Zarei
A
Dehbashi
S
Ghasempour Shirazi
L
Rajaeefard
A
Schmidt
E.H
Journal Article
2022
03
05
<strong>Objective: </strong>To determine if sectioning of cervical septum in hysteroscopic metroplasty of the complete uterine septum is associated with intra-operative bleeding, cervical incompetence, and secondary infertility. Design: Multicentric, randomized, controlled clinical trial. Setting: University hospitals. Patients: Twenty-eight women with the diagnosis of complete uterine septum who had history of pregnancy wastage or infertility. They were randomized into two groups; Group A; underwent metroplasty including section of the cervical septum.Group B: Underwent the same procedure with preservation of the cervical septum. Interventions: Hysteroscopic metroplasty performed for all patients in the two groups. Main outcome measures: Operating time, Distending media deficit, total distending media used, intra-operative bleeding, complications, and reproductive outcome. Results: Operating times were 36.40±10.67 minutes and 73±14.40 minutes in group A and group B respectively (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-90:Dendritic Cells And Pregnancy:A Bidirectional Relationship ToProtect The Semiallogenic Fetus
250023
EN
Moazzeni
S.M
Journal Article
2022
03
05
Dendritic cells (DCs) constitute a system of antigen presenting cells (APC) that are key regulators of immune responses. DCs are not only critical for induction of primary immune responses, but depending on their subsets and their maturation state may also be important for the induction of immunological tolerance, as well as for the regulation of T cell – mediated cytokine profiles. Regarding the importance of Th2 immunity as well as induction of tolerance to fetal allograft during pregnancy and having considered the mutual role of DCs in induction of immunity vs. tolerance and Th1 vs. Th2 immune responses, it seems that these cells could be potentially suitable candidate that mediate the balance of maternal immune responses to support pregnancy. To address this issue we set out a series of studies concerning the role of DCs in indispensable immuneregulation during successful pregnancy and the effects of pregnancy related soluble factors (hormones, cytokines etc.) on DCs. First of all we evaluated the frequency, localization and immunophenotype of murine endometrial DCs during different stages of estrous cycle. In addition, to address the systemic effect of hormonal fluctuations during estrous cycle, the same variables were studied in splenic DCs. The second study was performed to evaluate the kinetics of endometrial DC subsets at different stages of murine pregnancy. To address the systemic effect of pregnancy on DC kinetic, the same variables were also studied in splenic DC populations. The third study was set out to show the effects of soluble factors released in the supernatant of murine decidual cell cultures on the capacity of dendritic cells to present antigens in-vivo and on their ability to induce cytokine production by primed lymphocytes. We also investigated the immunosuppressive effect of pregnant mouse serum on allostimulatory activity of DCs to determine how activity of DCs could be affected during pregnancy. Regarding the pivotal role of Indoleamine 2, 3 dioxygenase (IDO) enzyme in pregnancy and considering that DCs are one of the main sources of this enzyme, the next studies were performed to investigate the effects of pregnant serum and decidual microenvironment on IDO induction in DCs and the role of IDO in DCs induced immunoregulation. Various methods including: cell separation methods (mostly splenic DCs and lymph node T cells), cell and tissue culture methods, Mixed leukocyte reaction(MLR), lymphocyte transformation test(LTT), ELISA, cytochemical and immunohistochemical methods, morphometric analysis, flow cytometry, HPLC etc. were used to perform these studies. Our data demonstrate that the balance of DCs subsets is finely tuned throughout estrous cycle and pregnancy. The frequency of endometrial DCs is highest at estrus, a phase in estrous cycle in which mating occurs and copulation is associated with further recruitment of large numbers of DCs in to the early deciduas, pointing an eminent immunoregulatory role of DCs in maintenance of pregnancy. It seems that soluble factors produced by decidual cells are important mediators of immunoregulation at the fetomaternal interface which provide the two fundamental requirements for protection of semiallogenic fetus, namely immunologic tolerance and predominance of Th2 immunity, through modulation of DCs function. Pretreatment of DCs with supernatant from decidual cultures but not pregnant serum significantly induces IDO expression while both treatments inhibit their capacity to induce production of TH1 cytokines and allogenic T cell allostimulatory capacity. Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 53 In conclusion, it can be postulated that DCs are key regulators of immune system during pregnancy and bidirectional relation of DCs and pregnancy protects the semiallogenic fetus from immunologic rejection. Such information may provide the basis for better understanding of female genital tract-immunity and may shed light on the way through which immunologically supporting microenvironment is organized for pregnancy maintenance.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-89:Expression Of MelatoninergicReceptors In Human Placenta AndChoriocarcinoma Cells
250024
EN
Lanoix
D
Ouellette
R
Villancourt
C
Journal Article
2022
03
05
<strong>Background:</strong> Melatonin concentrations increase in maternal blood during pregnancy, reaching a maximum at term. Moreover, experimental data suggest a possible influence of melatonin on placental function and fetal development in humans. To date, the expression and role of melatonin receptors in human placenta choriocarcinoma cell lines and in human term placental tissues remain to be elucidated. METHODS AND Materials and Result: Results from RT–PCR, western blotting and confocal microscopy demonstrated that the MT1, MT2 and RORa1 melatonin receptors are expressed in the human term placental tissues and in choriocarcinoma cell lines JEG-3 and BeWo (both in vitro models of human trophoblast). Furthermore, enzyme-linked immunosorbent assay showed that 6-chloromelatonin (a melatonin agonist) inhibits, in a dosedependent manner, forskolin-stimulated hCG- β secretion in JEG-3 (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-81:Management Of Hirsutism
250025
EN
Cianci
A
Farina
M
Journal Article
2022
03
05
Androgens constitute an important physiologic component for the female, representing the principal precursors of estrogens and directly participating to the secondary sexual characters determinism and to the sexual identity and behaviour definition. Androgens play several and important metabolic effects, acting, moreover, in the regulation of a number of the female organism functions. A regular and adequate ovarian and adrenal production of that hormonal fraction is essential for the activation and the modulation of important physiologic functions as: 1) Maintaining and regulation of the normal steroidogenic processes, acting as fundamental precursors for the global production of sexual steroids; 2) Induction of the process of follicular atresia following the selection of the dominant follicle; 3) Trophism of some tissues (especially of skin, bone and muscles); 4) Modulation of libido and sexual procectivity, in synergism with psycho-emotive and socialbehavioural factors. However, a condition of hyperandrogenism, due to excess of the steroid production or to increase of its biological activity, may determinate serious negative consequences which, often, impair not only the aesthetic aspect but also the psychic area and the interpersonal relationships. Not less important are the repercussions on the metabolic asset and, in successive phases of the life, on the cardiovascular risk. That situation appears more and more important considering that up to 10% of adolescent women complain with a symptomatology related to an excessive androgen activity, even if the correct prevalence of the phenomenon could be largely affected by the diagnostic criteria utilized. With the term of “hyperandrogenism” are identified all these clinical-pathologic conditions characterized by the presence of over-physiologic levels of androgenic steroids and/or the increased receptor sensitivity at the level of target tissues with consequent exaltation of the final biological effect . On the other hand, with “hyperandrogenic state” are identified all these clinicalpathologic conditions, with multifactorial aetiology characterized by: 1) Increased body hair growth in normally smooth skin areas and/or with only little hair follicles (face, thorax, abdomen, gluteus, alba line) and increased growth speed of the hair structures; 2) Increased sebaceous production of the skin (in areas normally rich in sebaceous glands) and at the level of the scalp; 3) Alopecia; 4) Variable degrees of insulin resistance and/or hyperinsulinemia; 5) Reduction of the circulating levels of HDL cholesterol and modification of its rate with total cholesterol; 6) Alteration of the normal processes of ovarian follicle genesis with increasing of follicular atresia and failure on the selection and dominance of pre-ovular follicle; increase of ovarian stroma, albuginea thickening and formation of follicular micro-cysts. Excluding neoplastic and hyatrogenic aetiology the so called functional causes of Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 48 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 hyperandrogenism are usually characterized by a multifactorial aetiopathogenesis. Policystic ovarian syndrome (PCOS) represents the most frequent cause of hyperandrogenism in post-pubertal age. This complex disendocriny, which presents chronicity and self-maintaing aspects, is characterized by a severe disarray of the principal intra- and extra-ovarian feed-back mechanisms, presenting with a wide clinical, morphological and endocrine-metabolic variability. Among all the causes of hyperandrogenism, PCOS is the most important under the etiophatogenic and prognostic aspect, often associating with characteristic dismetabolisms as dislipidemia (low levels of HDL cholesterol, alteration of total cholesterol/HDL cholesterol rate), impaired carbohydrates tolerance, insulin-resistance, overweight and obesity. According to that condition several therapeutic options have been proposed over the years. General measures are represented by eliminate causative factors, optimizing weight and manage hair with bleaching, cutting or shaving, electrolysis, laser epilation, but nowadays the most utilized and efficacious are represented by the etiphatogenetic therapy: 1) Ovarian andorgens production inhibitors (oral contraceptives, GnRH analogues); 2) Androgens receptor antagonists (ciproterone acetate, flutamide); 3) 5-alpha-reductase inhibitors (finasteride, dutasteride); 4) Insulin sensitizers (metformin, acarbose). Oral contraceptives can be administered alone or in association with antiandrogens, insulin sensitizers or GnRH analogues. Ciproterone acetate has been largely utilized with great success. It can be administered in low dosage being effective on acne/seborrhoea more than in hirsutism. The inverted sequential regimen according to Hammerstein, in association with low dose of estrogens, seems to be effective with Ferriman & Gallway hirsutism scores higher than 15, in presence of androgenic alopecia or impaired tolerance to estradiol (>20mcg). Low dosage of ciproterone acetate in association with GnRH analogues are often utilized in the treatment of poor responsive women in assisted reproduction protocols. Finally, low dosage in association with estrogens show same effectiveness vs GnRHA characterizing as secondary choice in severe cases, for the absence of pituitary suppression. Long term administration of ciproterone acetate plus contraceptive pill may, however, determine side effects as headache, nausea, depression, weight increase, hepatic function impairment and increase in triglycerides blood level. More innovative appears the approach with insulin sensitizers. Several research demonstrated the key pathogenetic role played by hyperinsulinemia in the ovarian androgen overproduction of PCOS. On that purpose the reduction of hyperinsulinemia ameliorate the androgen excess.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-79:Polycystic Ovary Syndrome:Disease Or Adaptation?
250026
EN
Berga
S.L
Journal Article
2022
03
05
The etiology of polycystic ovary syndrome (PCOS) remains uncertain. The three most commonly accepted causes are: a metabolic alteration that results in insulin resistance; an enzymatic defect in the steroidogenic pathway that increases androgen production; and a primary defect of the hypothalamic GnRH pulse generator that increases LH and decreases FSH. Some have argued that PCOS is invariably linked to insulin resistance, but this explanation does not fully account for the gonadotropin aberrations and resulting anovulation that are so characteristic of this syndrome. Part of the uncertainty regarding the etiology of PCOS relates to its variable presentation. For instance, thin women with PCOS are less likely to display insulin resistance and more likely to have excessive androgen levels. If ultrasound criteria are used to make the diagnosis of PCOS, even thin, eumenorrheic women who are ovulatory may be diagnosed as having PCOS. Our research objective has been to determine the etiology of the anovulation characteristic of PCOS so as to refine therapeutic approaches. In an effort to find a parsimonious explanation, we are seeking to determine the role of insulin resistance and hyperandrogenism in the genesis of the gonadotropin aberrations characteristic of PCOS. Indeed, available data suggest that both androgens and insulin increase GnRH drive and that increased GnRH drive secondarily reduces FSH to levels insufficient to fully support folliculogenesis to the point of ovulation. These data argue for more than one cause of PCOS. These insights form the basis for individualizing treatment approaches. For those not seeking immediate fertility, common treatments include anti-androgens, insulin action modifiers, metformin, and oral contraceptives alone or in combination. For those seeking fertility, the use of metformin alone or in combination has been advocated. A recent randomized trial suggested that clomiphene was superior to metformin for the treatment of infertility due to PCOS (Legro RS et al. New Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 47 Engl J Med 2007; 356: 551). Treatment efficacy varies widely and likely reflects etiologic nonhomogeneity. Recent advances in our understanding of the link between metabolism and aging suggest that PCOS may represent an adaptation to low fuel environments. If so, then it will be especially important to focus on nutritional counseling as a means of ameliorating symptom progression.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-78:Stress-Induced Anovulation
250027
EN
Berga
S.L
Journal Article
2022
03
05
Functional hypothalamic amenorrhea (FHA) is characterized by a reversible reduction in GnRH drive and mild hypercortisolemia. The behavioral antecedents leading to the development of non-organic forms of chronic hypothalamic anovulation and amenorrhea are variable. Dieting and excessive exercise are often initiated to cope with subtle psychogenic challenges. It is accepted that what is deemed stressful is to a large extent idiosyncratic and individualistic. Further, women with FHA rarely report a single isolated stressor and FHA can develop in the absence of significant metabolic imbalance. We wondered, therefore, if metabolic and psychogenic stressors would be more deleterious to reproductive function when combined. We developed a monkey model to test the hypothesis that a combination of metabolic and psychosocial stressors would synergistically disrupt reproductive function. We recognized that the distinction between psychogenic and metabolic stress was, to some extent, artificial, because the corresponding increase in cortisol secretion that accompanies activation of the limbichypothalamic- pituitary-adrenal axis elicits obligatory metabolic adaptations regardless of initiating cause. However, therapeutic recommendations are often based on the notion that metabolic stressors are easier to identify and ameliorate than psychogenic ones, so it seemed important to try to determine the impact of metabolic versus psychosocial stressors alone and then in combination. To date, our monkey data suggest that mild metabolic imbalance heightens reactivity to subsequent psychogenic challenge. Other investigators have shown that psychogenic stress heightens the impact of metabolic (exercise) challenge. However, the neural concomitants that transduce either metabolic or psychogenic stress into altered neuroendocrine secretory patterns or heighten Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 46 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 reactivity to mildly stressful events remain poorly defined. Our work in female cynomolgus monkeys suggests that the serotonergic axis differs in stress-sensitive and stress-resistant monkeys. We found that cerebrospinal fluid levels of CRH were comparable in FHA and eumenorrheic women, but that, contrary to expectations, beta-endorphin levels were lower in women with established FHA. Interestingly, CSF levels of cortisol were higher in FHA. If the set point for inhibition of the HPA axis were comparable in FHA and eumenorrheic women, one would expect that CSF CRH levels would be lower in FHA than in eumenorrheic women. Thus, our data suggest that women with FHA display central resistance to the negative inhibitory feedback effects of cortisol. The physiological, cellular, or molecular basis of this stress sensitivity and limbic-hypothalamic-pituitary-adrenal feedback insensitivity in FHA has not been identified, but neurotransmitter systems such as serotonin and GABA remain as candidates. FHA is, in theory, reversible. Based on our understanding of the psychological and behavioral correlates of FHA, we developed a 16-session program of cognitive behavior therapy (CBT) for women with FHA. We did not instruct women to alter exercise or diet habits, but we did address attitudes that underlie stress sensitivity. Women with FHA were randomized to CBT or observation. Over 85% of women treated with CBT recovered either full or partial ovarian function as assessed by weekly estradiol and progesterone levels, whereas only 25% of those randomized to observation displayed reproductive recovery. Those who recovered did not gain weight. In aggregate, our data suggest that metabolic variables alone are rarely the primary cause of FHA and that psychological care has the potential to reverse FHA and thereby spare women with FHA the invasive, risky, and costly procedures of ovulation induction or assisted reproduction.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-77:Fetal Consequences OfOccult Maternal Conditions
250028
EN
Berga
S.L
Journal Article
2022
03
05
It is a medical truism that fetal health depends on maternal health. Several conditions such as adult diabetes and cardiovascular disease have been linked to fetal exposure to poor maternal health and maternal stress. These insights have sparked investigation into the fetal origins of adult disease (FOAD). When the maternal conditions that are recognized to confer fetal consequences are amenable to medical intervention, then consideration should be given to identifying and correcting these conditions prior to conception. This is especially important when conception requires medical assistance, fetal consequences are significant, when screening strategies exist that are readily available and reliable, or when the condition is infectious and preventable by vaccine. The task of ensuring fetal health is more difficult when the maternal conditions are occult. The mechanisms by which fetal development is altered by maternal conditions are many and include epigenetic mechanisms that alter DNA methylation patterns and therefore have the potential to be multigenerational. A short list of occult maternal conditions for which screening might be of benefit includes maternal thrombophilias, impaired maternal glucose tolerance, stress, hypothyroidism, and nutritional deficiencies, especially those that cause folate deficiency. For instance, celiac disease may cause malabsorption, hyper- or hypothyroidism, and infertility due to oligo-ovulation. Newer diagnostic techniques allow us to screen for celiac disease noninvasively and it is important to consider Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 45 screening for celiac disease in women with infertility. Another common but often occult maternal cause of compromised fetal development is hypothyroidism. Worldwide, goiter due to iodine deficiency is a common cause of hypothyroidism and cretinism. Other common causes of maternal hypothyroidism include autoimmune etiologies, including celiac disease, and stress. The mother is the sole source of thyroxine in early gestation and the predominant source during the entire gestation. Women with a known cause of thyroid deficiency due to organic conditions such as Grave’s disease or Hashimoto’s thyroiditis require an increase in thyroxine dose very early in gestation. Alexander EK et al showed that the mean thyroxine requirement increased about 50% by week 8 of gestation (New Engl J Med 2004; 351:241). Thyroxine requirements appear to be greater in women undergoing assisted reproduction and ovulation induction and in those with a multiple gestation. The primary consequence of inadequate fetal exposure to thyroxine is compromised neurodevelopment. There are many important genes that are regulated by thyroxine and this appears to be the mechanism by which inadequate thyroxine exposure compromises fetal neurodevelopment. Since psychosocial stress and inadequate nutrition compromise hypothalamic TRH drive and lead to subtle forms of functional hypothalamic hypothyroidism, it may be more important than previously recognized to mitigate poor maternal psychosocial and nutritional compromise. In summary, recent data on the importance of thyroxine for fetal neurodevelopment support the notion that there should be a high index of suspicion and a low threshold for screening for thyroid disease. Optimally, screening should occur before conception and should include TSH, free thyroxine, and possibly anti-thyroid antibodies such as TSI (thyroid stimulating immunoglobulin) and TPO (thyroid peroxidase). It is more difficult to screen for functional forms of hypothyroidism that accompany psychosocial and metabolic stress and thus amelioration of psychosocial and nutritional stress should receive high priority.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-76:Comparison Of The Efficacy Ofthe Aromatase Inhibitor Letrozoleand Clomiphene Citrate As Adjuvantsto Recombinant Follicle-StimulatingHormone In Controlled OvarianHyperstimulation: A Prospective,Randomized, Blinded Clinical Tria
250029
EN
Barroso
G
Menocal
G
Felix
H
Rojas-Ruiz
JC
Aslan
M
Oehninger
S
Journal Article
2022
03
05
<strong>Objective: </strong>To study the efficacy of the aromatase inhibitor letrozole as adjuvant to recombinant FSH (rFSH) in controlled ovarian hyperstimulation (COH). DESIGN: Prospective, randomized, and blinded clinical study. Setting: Academic tertiary institute. Patients: Forty-one patients with unexplained infertility undergoing intrauterine insemination (IUI) therapy were randomized to receive either letrozole or clomiphene citrate (CC) as adjuvants to rFSH. Interventions: From day 3 to 7 of the cycle 2.5 mg/d letrozole or 100 mg/d CC were administrated. All patients received 75 IU rFSH starting on day 7 of stimulation until the day of hCG administration. Ovulation was triggered with recombinant hCG (250 microg) when the leading follicle(s) reached 18 mm in diameter. A single IUI was performed 36 hours later. The luteal phase was supplemented with micronized progesterone vaginally. Main Outcome Measures: Ovarian stimulation response (E(2) levels and number of follicles) was our primary outcome.
<strong>Results: </strong>There were no differences in demographic characteristics between groups. Although there was a significantly lower peak serum E(2) level in the group receiving letrozole + rFSH compared with CC + rFSH (914+/-187 vs. 1,207+/-309 pg/mL, respectively; p16 mm) preovulatory follicles. A significantly higher endometrial thickness was observed at the time of hCG administration in patients that received letrozole (9.5 +/- 1.5 mm vs. 7.3 +/- 1.1 mm; p=0.0001). The clinical pregnancy rate was similar between groups (23.8% vs. 20%, respectively).
<strong>Conclusion: </strong>The aromatase inhibitor letrozole appears to constitute a good alternative to CC in patients with unexplained infertility undergoing gonadotropin-stimulated COH cycles combined with IUI therapy.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-75:Menopur® Vs Gonal-F® InIran: A Cost Effectiveness AnalysisBased On MERiT Study
250030
EN
Al-Inany
H
Journal Article
2022
03
05
Multifollicular development via Gonadotrophin administration is still an integral component for ovarian stimulation in IVF/ ICSI cycles1. The technological developments of pharmaceutical Gonadotrophins over the last 40 years have shown improvements in specific activity, purity, degradation and impurities2 and recently with the introduction of Highly Purified Menotropins (HP- hMG), both effectiveness and cost effectiveness should be considered together to aid the judgment about whether one drug should be preferred to a comparator3. To examine the cost effectiveness of rec-FSH versus HP-hMG in Iran, it was necessary to build a model that simulates the IVF treatment cycle with its key steps Because of the recurring nature of IVF/ ICSI cycles, it was decided to run a model called Markov analysis model for three successive cycles. The transition probabilities for different health states used in the present analysis were based on results from a recently published randomized controlled trial called MERiT®4,5, for estimation of the cost of live birth in IVF/ICSI cycles. In the case of using rec-FSH, the live birth rate is 31.8%, while when using MENOPUR®, the live birth is 45.2% with an incremental cost effectiveness ratio ICER of 12,506,340 IR ~ 1050 EURO per life birth in favor of MENOPUR®. Cost effectiveness analysis reveals that MENOPUR® is better leading to Live birth rate state than that of rec- FSH
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-74:Pentoxifylline Therapy After Laparoscopic Surgery For Different Stages Of Endometriosis, AProspective, Double Blind,Radomized, Placebo-ControlledStudy
250031
EN
Alborzi
S
Ghotbi
S
Parsanezhad
M.E
Dehbashi
S
Alborzi
M
Journal Article
2022
03
05
<strong>Objective:</strong> To evaluate the effects of pentoxifylline administration on the patients with different stages of endometriosis, for whom laparascopy was performed. Design: prospective, double-blind, randomized, placebo-controlled clinical. Design Classification: Canadian Task Force classification І. Setting: University and private hospitals. Patients: Eighty eight women, all with infertility, some with dysmenorrhea, dyspareunia, or pelvic pain, for whom laparascopic diagnosis of endometriosis was done and as the principal part of the treatment, appropriate surgery was carried out. Interventions: The treatment group received 800 mg of pentoxifylline daily for six months immediately after surgery. The control group received placebo capsules. All of them were followed for one year thereafter. Measurements and Main Results: A comparison of pregnancy rate and recurrence of signs and symptoms in the two mentioned groups was done. Forty three patients were studied in the pentoxifylline group, and forty five in the placebo one. The cumulative pregnancy rate was 39.5% and 35.6% in the treatment and control groups, respectively. The overall recurrence of signs and symptoms was 14% in the former group and 15.6% in the latter one. So there were no statistically significant differences between the two groups in the rates of pregnancy and recurrence (P values: .7 and .832, respectively). Neither there was any significant statistical difference between the same stages in the two groups regarding immunomodulation. Conclusions: According to the results of this study, and while keeping in mind that appropriate and perfect operation is the main aspect of the endometriosis treatment, there is no evidence that immunomodulation with pentoxifylline aids fertility, or recurrence of signs and symptoms in women with different stages of endometriosis (i.e., minimal, mild, moderate, or severe).
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-73:Microscopic Surgery Of Fallopian Tube Using Laparoscope
250032
EN
Alborzi
S
Journal Article
2022
03
05
One of the main causes of infertility is tubal factor. Post surgical adhesions, sexual transmitted diseases, PID, and post partum infection, may lead to tubal and peritubal factor infertility. Surgical approaches to these problems were not successful and after introduction of IVF in the field of reproductive medicine, trend of most infertility specialists is toward ART. In early 1980s, the introduction of microsurgery by laparatomy has created new hopes for these patients. With advances in operative laparoscopy during the 90s surgeons shifted from laparatomy to laparoscopy for patients who needed an operation in abdomen and pelvis. However, microsurgery by laparoscopy is a difficult task and there are not many surgeons in the world that could perform this technique. Since 2003, we have started using laparoscopic microsurgery to treat tubal and peritubal factor infertility. Different surgical techniques such as end to end anastomosis, corneul anastomosis, salpingoovolysis, salpingoneostomy and fimbrioplasty, has been done by laparoscopic microsurgery. Here indications, surgical techniques, and success rates will be discussed and some videos of our operations will be demonstrated.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Donation And Surrogacy From Islamic View
250033
EN
Merghati
S.T
Omani Samani
R
Journal Article
2022
03
05
<strong>Introduction:</strong> So far, there are too many children are born by donation protocols or surrogacy. In Islamic countries upon the third conference of the Islamic Fiqh Council in 1986, all kinds of donations are banned. So, no Islamic country practices donations except Iran in which, all of them are practiced everyday. As Iran represents an Islamic country and most of the Iranian people are Muslims, there can be some social, legal and psychological concerns for the children born by these protocols that should be considered, discussed and clarified for avoiding future complications. This study outlines some proofs and documents for helping the Islamic law-makers, clergy leaders and professionals to pursue a practical guideline in this regard and maybe an upgrade to the previous statement.
<strong>Materials and Methods:</strong> As we can not find the equivalent terms in the Islamic resource, we review the Islamic resources mostly the Holy Quran to find some proofs and we could find out the Islamic ideas. Results: 1) These protocols are compared with adultery in many papers and statements but it has been shown that they are totally different. 2) It has been stated that these protocols bring confusion of lineage that does not. 3) Some relations like intimacy have been discussed that is OK in these families. 4) Two vision of cell donation and multiple marriages have been discussed and proved that cell donation vision is accepted and no fake marriage is OK in Islam. 5) We suggest twomother theory that clarifies these children lineage so other rights linked to the lineage upon Islam. 6) We underline the anonymity of the donor to avoid complication that may come if the donor identity is known, that upon Islam donors will be true parents also, and some rights goes back to them. 7) We have suggestions about the inheritance of the children born by sperm and embryo donation which can not be solved by ordinary Islamic law. <strong>Conclusion:</strong> It has been shown that Islam can accept donation and surrogacy by presenting proofs and documents from Islamic resources and clergy leaders can pursue a practical guideline in this regard.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
O-72:A Comparison Of FollicularResponse Of Ovaries To OvulationInduction After LaparoscopicOvarian Cystectomy Or Fenestrationand Coagulation Versus NormalOvaries In Patients WithEndometrioma
250034
EN
Alborzi
S
Ravanbakhsh
R
Parsanezhad
M.E
Alborzi
M
Dehbashi
S
Journal Article
2022
03
05
<strong>Objective: </strong>To compare follicular response to controlled ovarian hyperstimulation (COH) between normal ovaries and ovaries previously treated by different laparoscopic techniques for ovarian endometrioma. Design: A prospective randomized clinical trial. Settings: University and private infertility clinic. Patients: 81 infertile patients with either unilateral or bilateral endometrioma. Interventions: For 65 patients with unilateral endometrioma, laparoscopic ovarian fenestration and coagulation was performed in 24 cases (group 1) and laparoscopic ovarian cystectomy in 41 others (group 2). In 16 patients who had bilateral endometrioma (group 3) cystectomy was done in one ovary and fenestration and coagulation in the contra-lateral side. All patients underwent COH with clomiphene-citrate and HMG in subsequent cycles. Main Outcome Measures: Number of follicles in each ovary of above patients after COH.
<strong>Results: </strong>Mean number of follicles in group 1 was 2.6 ±1.6 in post fenestration and coagulation ovaries and 2.8±1.6 in normal ovaries. These figures were 3.2±1.1 in postcystectomy ovaries and 3.2±1.7 in normal ovaries in group 2.In group 3 number of follicle was 2.9±1.1 in post-cystectomy ovaries and 3.05±1.3 after fenestration and coagulation. There was no statistical significant difference between these figures.
<strong>Conclusions:</strong> Response of ovaries to COH after laparoscopic ovarian cystectomy or fenestration and coagulation was the same. There was no difference in response to COH between normal ovaries and those operated by laparoscopic techniques mentioned above.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Gender Preferences AndDemand For Sex Selection: A SurveyAmong Pregnant Women InPakistan
250035
EN
Zubair
F
Dahl
E
Sher Shah
S
Ahmed
M
Brosig
B
Journal Article
2022
03
05
<strong>Background: </strong>In its recent report “Human Reproductive Technologies and the Law”, the House of Commons’ Select Committee on Science and Technology called for greater efforts to establish the potential demographic impact of sex selection across all sectors of UK society. Given the well-known preference for boys over girls among some communities, there is concern that a readily available service for social sex selection may upset the balance of the sexes. Of particular interest are the gen-der preferences and the demand for sex selection among Pakistanis.
<strong>Materials and Methods: </strong>We conducted a social survey on gender preferences and demand for preconception sex selection among 301 pregnant women in Karachi, Pakistan, using a self-report ques-tionnaire consisting of 13 questions.
<strong>Results:</strong> 41.5% wish to have a family with an equal number of boys and girls. 3.3% would like to have only boys, 1.0% only girls, 27.6% more boys than girls, 4.3% more girls than boys, and 22.3% stated that they do not care about the sex composition of their family. While 6.3% could imagine em-ploying cytometric sperm separation for social sex selection, 76.1% could not; 17.6% were undecided. 27.2% felt that social sex selection ought to be legal, 48.8% thought it ought to be illegal, and 23.9% were undecided. Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 42 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007
<strong>Conclusion:</strong> Although Pakistani women do show a marked preference for boys over girls, the number of women will-ing to subject them selves to cytometric sperm separation appears to be too small to cause a severe imbalance of the sexes. However, further research among British citizens of Pakistani origin is needed to establish whether or not sex selection poses a serious threat to the sex ratio of UK communities.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Life-Table Analysis OfArtificial Insemination PregnancyRates For Couples With Male Factorand Idiopathic Infertility
250036
EN
Suzuki
K
Kasai
T
Suzuki
M
Miyake
M
Fujie
M
Mizuno
K
Hirata
Sh
Hoshi
K
Journal Article
2022
03
05
<strong>Background: </strong>In the summer of 2002, standard guidelines for the application of assisted reproductive technology were reported by a research group of the Ministry of Health, Labor and Welfare. The present study aimed to examine the relationship between the number of cycles of artificial insemination and the cumulative pregnancy rates according to the cause of infertility.
<strong>Materials and Methods:</strong> Patients who experienced their first cycle of artificial insemination during the period of January 1999- December 2002 were included in the study and were divided into a male factor infertility group and an idiopathic infertility group. Cumulative pregnancy rates resulting from artificial insemination with the husband's semen were calculated by the life-table approach.
<strong>Results:</strong> During the study period, 139 couples entered the assisted reproduction program and underwent 581 cycles. Significant differences were observed in cumulative pregnancy rates between the two groups.
<strong>Conclusion:</strong> It is recommended that couples with male factor infertility and who fail to conceive within six or seven cycles of intrauterine insemination, consider a modification of treatment strategy such as in vitro fertilization, because cumulative pregnancy rates of this group were reached at a plateau within six or seven cycles. In contrast, patients with idiopathic infertility, the cumulative pregnancy rates appeared to increase constantly with each subsequent cycle. It is important to consider modifications of treatment strategy in the light of the cause of infertility.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Analysis Of National Representative Opinion SurveysConcerning Gestational Surrogacy InJapan
250037
EN
Suzuki
K
Hoshi
K
Minai
J
Yanaihara
T
Takeda
Y
Yamagata
Z
Journal Article
2022
03
05
<strong>Objective: </strong>Although gestational surrogacy offers several advantages, this procedure has given rise to some ethical and legal issues.We aimed to clarify the factors affecting the attitude of the Japanese toward gestational surrogacy. Design: Cross-sectional study. Setting and participants: Nationwide opinion surveys concerning assisted reproductive technologies (ART) were carried out in 1999 and 2003. Participants included 2568 and 3647 people from the general public surveyed in 1999 and 2003, respectively (1564 people received only the questionnaire, and 2083 people received a questionnaire and brochure about ART). Main outcome measure: Multivariateadjusted odds ratio and 95% confidence interval from logistic regression models for factors affecting the attitude toward gestational surrogacy. Results: In both surveys, approximately half of respondents approved of gestational surrogacy; 20–30% disapproved of the procedure. People with high socioeconomic status clearly expressed their opinion on this issue. A liberal attitude toward gender role promoted approval of gestational surrogacy; a liberal attitude toward family had the opposite effect. Conclusion: Our findings suggest that socioeconomic status affects people’s Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 41 expression of their opinion regarding this issue, while attitudes toward this procedure were influenced by individual belief. Considering socioeconomic status and diversity of individual belief is required for further discussion on this topic.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Comparison Between TheScientific And Koranic Explanationfor Human Creation
250038
EN
Shaban
J
Journal Article
2022
03
05
People have always been interested in knowing how they originated, and how they were born. Ancient people, filled with curiosity, developed many answers to these questions. All of what we know now about the development of the embryo within the uterus is based on the accumulated scientific knowledge achieved over the centuries and particularly due to advanced researches in resent years after the development of the modern technology. Scattered through out the Koran are many statements about embryology. The progress of embryology through the ages from the earliest records to the present and Enlightenment in this field in the holy Koran and Hadith are discussed. Read in the name of your Lord who created. Created man from a clot. (Al alaq 2-1) This is the first Aya in the Holy Koran and this is the first stage in human Creation. In the Holy Koran God gave, 14 centering ago a detailed description of human creation from clay, ending with death, then resurrection in many verses (Aya). Conclusion: The holy Koran was the first to exactly describe human development 14 centuries ago, long before the discipline of Embryology was even established. Only in the last century, modern science was able to prove in detail and with evidence what was reveled, in TRUTH, in the Holy Koran.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Health Seeking Behavior OfCouples With Secondary Infertility
250039
EN
Sami
N
Saeed Ali
T
Journal Article
2022
03
05
<strong>Objective:</strong> To determine the factors affecting the health-seeking behavior of couples with secondary infertility in Karachi. Design: A descriptive case series. Place and Duration of Study: The data was collected from women attending infertility clinics in five tertiary care hospitals in Karachi from March to June 2003. Patients and Methods: All currently married women, between the age of 15-35 years, with at least one previous conception, irrespective of outcome, attending an infertility clinic and consenting to participate in the study, were included. Women with corrective surgery on vagina and uterus, and cases of primary infertility, were excluded. Multiple logistic regression models were used to determine the association of various factors, affecting the health-seeking behavior, with statistical significance set at p < 0.05 for the covariates and the interaction terms between various factors. Results: The women consulted multiple health care providers for treatment of secondary infertility. The main reasons for seeking treatment were couple’s wish (54.2%), family pressure (22.6%) and want of a son by husbands or in-laws (20.4%). The most commonly sought providers were physicians (74.7%), Traditional Birth Attendants (TBA, 39.5%), Spiritual healers (26%), Hakeems (23%) and Homeopaths (17.2%). Most of the women who consulted non-physicians were illiterate (69.4%) as compared to those who consulted a physician (37.8%, p-value = 0.00). The non-physicians were more commonly consulted by women Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 40 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 belonging to low socioeconomic group. The posttreatment complications were more common among women who consulted nonphysicians. <strong>Conclusion:</strong> Pressure from husbands and inlaws compels women for consulting multiple providers. Health seeking behavior for infertility is affected by the literacy and socioeconomic status of the women.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Payment, Contracts AndRegistration In Oocyte And SpermDonation
250040
EN
Omani Samani
R
Merghati
S.T
Journal Article
2022
03
05
Today many children are born by ART and also a part of them by egg, sperm and embryo donation or surrogacy. There is a need for some guidelines to outline these children’s rights and prevent future problem. These guidelines can not be totally universal because of cultural, religious and ethnical differences. As one of the most important part of donation and surrogacy is payment, which brings other problems and questions like: Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 39 human dignity, advertisement, contracts, registration, law and human rights. Here we discussed the payment, contract and registration upon years of experience in kidney transplantation from living donor in Iran, which had faced similar problem and solved a large part of them. Here are our results: 1) nobody can sell their body’s part and it is against human dignity, so, all of these procedures should be called donation. 2) the payment should be considered as compensation of the donor for their time, drug usage, operation, lab tests… 3) as there is no business on human parts, there can not be a contract on donation, but a contract on compensation is OK. 4) governmental law can not make anyone to donate their body cells, but registration committee can prevent repeat of any contract cancellation by not accepting a donor who had cancelled a contract before. 5) a governmental registration center shall be established to register donors and recipients’ data and save them for preventing future complication, we insist on anonymity of the donors and keep the privacy of the files. 6) registration center should be responsible for contract, payment and data protection, that our suggestion is to design computer network enabling each town to have a registration center. 7) there shall be a governmental law to prevent any advertisement or need notice on the walls of the centers or in newspapers and magazines.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Is There A Limit To TheNumber Of Embryos To BeTransferred/Created For ART?
250041
EN
Milliez
J.M
Journal Article
2022
03
05
Pregnancy rate after ART (Artificial Reproductive Technology) is abusively considered to increase in proportion of the number of embryos transferred. Indeed this is only true up to less than 5 embryos, and is highly dependant on the woman’s age. Above all a policy of too many embryos transfer entails an excessively high number of multiple pregnancy, as high as 26-30%, including 3- 4% triplets. The hazards of multiple pregnancy both for mothers and offspring led several countries to limit by law the number of embryos to be created/transferred during ART. A policy of a single embryo transfer, eventually followed, in case of implantation failure, by the transfer of a cryopreserved embryo during a next cycle, has proven to be significantly as efficient as the transfer of two fresh embryos, with a drastic reduction in multiple pregnancy. The advantage of the transfer of a single embryo at the blastocyst stage is discussed. FIGO Ethics Committee recommends an embryo policy transfer achieving a singleton pregnancy.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Stem Cell Research: Ethicaland Religious Issues
250043
EN
Larijani
B
Journal Article
2022
03
05
<strong>Introduction:</strong> Stem cells are undifferentiated, primitive cells with the unrivalled ability to differentiate into any specialized cell type. The discovery of stem cells early in the 1980s has promised new treatments and possible cures for many debilitating diseases and injuries. Research using embryonic stem cells (ESCs) is a very important area of current biomedical investigation. Iran is one of the first countries which have produced human embryonic stem cells. However, stem cell research have raised a series of ethical and religious questions that are being confronted by multiple international organizations, nations, cultures, and religious traditions. Our aim is to review the main ethical issues in this challengeable field, considering the different religious viewpoints. The national specific guideline for the gamete and embryo research (2005) will be stated.
<strong>Materials and Methods:</strong> For compiling the article, we have searched articles in Google and Ovid search engines, PubMed, and IranMedex sources by using appropriate Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 37 keywords. We have also used the Holy Koran and religious opinions from great Muslim scholars. We have referred to some English, Arabic and Farsi books in this field.
<strong>Results:</strong> Stem cells can be obtained from different sources. Using adult stem cells is relatively free of ethical conflicts. But embryonic stem cell (ESC) research is controversial because harvesting the stem cells destroys the embryo. The status of the pre-implantation embryo is the most sensitive and disputed point in the debate on isolation of human ESCs for research. Considerable differences of opinion exist with regard to the ontological and moral status of the preimplantation embryo. On the other hand, many people believe that permitting this type of research paves the way for reproduction of an entire human and will open the way to a slippery slope of dehumanizing practices, such as embryo farms, cloned babies, the use of foetuses for spare parts, and the commodification of human life. The issue of ''Proportionality'', ''Justice'', ''Resource Allocation'' and ''Subsidiarity'' are a number of other important debates in this field. There is no consensus on the morality of the embryo, even within particular religious traditions. In Islam the embryo, even in the first day of its existence, has the right of life but according to some decrees (Fatwa), the use of embryo for therapeutic or research purposes may be acceptable under necessity if that is takes place before the point at which the embryo is ensouled. Conclusion: Research involving human embryos could be permitted for therapeutic purposes with full considerations and all precautions. Cooperation of scientists, ethicists, jurisprudents and lawyers is essential for establishing a culturally-adapted and well-controlled system at national and international level. Public education and information about the ethical issues raised by stem cell research and its application is necessary.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Attitudes Of Turkish AndIranian People Towards EggDonation
250044
EN
Khalili
M.A
Isikoglu
M
Senol
Y
Journal Article
2022
03
05
<strong>Introduction:</strong> We conducted two seperate descriptive studies in order to reveal the General attitudes of Turkish and Iranian people toward various aspects of oocyte donation (OD). Materials and Methods: The first Study was carried out in two seperate districts of the city of Antalya, Turkey. 400 particioants were chosen by dluster sampling method. The questionnaires were performed by 4th year medical students face to face with the participants. The second study was conducted in Isfahan city, Iran. Two hundred adults (Christians, n=100; Muslims, n=100) were asked to fill out the questionnaires. Results: The Turkish participants consisted of 232 women and 168 men. 64.75% were married, 5% were divorced. 63.75% had children, 15(3.75%) had infertility problems, 263(65.75)% were graduates of high school or university. Approval of egg donation was high in this study sample. Only 61 (15.25%) respondents showed complete objection againts egg donation and more men were in favor. Less than half of the participants think that their religion would prevent egg donation if they need it. More than half of the participants would prefer the use of egg donation treatment rather than adopting a child. For the Iranian participants, 53% of Christians and 69% of Muslims were married. The vast majority of the subjects had formal education; only 3 of the participants were illiterate. Only one-third had complete objection toward OD program. 68% and 43% of the Christians and Muslims were unaware of their religious attitudes on OD, respectively. Most of participants believed in informing the general public about OD in mass media. In addition, nearly half of the participants were in favor of OD over adopting a child. Psychological counseling was recommended by majority of respondents for both donor and the recipient of the eggs. Discussion: These two studies reveal the first data on the attitudes towards OD from countries whose population is mainly muslim. The most important conclusion is the fact that most of the participants do not have any objection against egg donation treatments. However, the vast majority did not know for whom the OD program is suitable. Therefore, the mass media should develop programs for informing the general public regarding the OD treatment program.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Social Sex Selection And TheBalance Of The Sexes: EmpiricalEvidence From Germany, The UKand The US
250045
EN
Dahl
E
Journal Article
2022
03
05
<strong>Introduction:</strong> Preconception sex selection for nonmedical reasons is one of the most controversial issues in bioethics today. The most powerful objection to social sex selection is based on the assumption that it may severely distort the natural sex ratio and lead to a socially disruptive imbalance of the sexes.
<strong>Materials and Methods: </strong>Based on representative social surveys conducted in Germany, the United Kingdom, and the United States, this paper argues that the fear of an impending sex ratio distortion is unfounded.
<strong>Conclusion:</strong> Given the predominant preference for a “gender balanced family”, a widely available service for social sex selection is highly unlikely to upset the balance of the sexes in Western societies.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Using Embryonic Stem Cellsin Therapeutic Research: ATheological Perspective
250046
EN
Aksoy
S
Journal Article
2022
03
05
Stem cell research is a recent technology that has been discussed by the scholars from various disciplines since it has different Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 35 dimensions that relates to these disciplines. Although the scientists in the field of biotechnology argues that this technology will be used for the benefit of the society it requires the legal, ethical, and scientific issues associated with this research be critically addressed and articulated. There is a necessity to distinguish two different types of stem cells, namely adult and embryonic origin. It is generally believed that it is morally less problematic to use adult stem cells, whereas there are some concerns, especially from theological perspective, to use embryonic stem cells in reproductive and therapeutic cloning. In this presentation we aim to address to the usage of embryonic stem cells for therapeutic purposes, and will argue that it is not only allowable to use embryonic stem cells for this purposes but also there are moral imperatives to use them since there is not enough concrete evidence to justify discontinuing the research on stem cell research. We discuss the issue from a theological perspective with special reference to Islam.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
The Beginning Of Human Lifeand The Embryos: A Philosophicaland Theological Perspective
250048
EN
Aksoy
S
Journal Article
2022
03
05
Life is a process which has a beginning and an end. Every health care professional, especially the one who “plays” more at these edges, should have a very clear position on when a human life begins and ends. This is very important, since the moral acceptability of some medical applications depends on the definition of these times. There are different views from the scholars in different academic fields on the time of the beginning of human life. Although there is not much debate on the “humanity” of a newborn, except some marginal philosophers, the moral status of the embryos and fetuses are still debated. In this presentation, it will be discussed whether determining the beginning and the end of life is a matter of moral or medical decisions. As it will immerge from the discussion that, it is a matter of moral decisions, the concept of human life will be defined from social sciences perspective, and the time of its beginning will try to be explored. The presentation will show that human life begins, therefore morally matters, in the womb at the beginning of 8th week after conception. After referring some bioethical concepts, like ‘human being’, ‘human person’ and ‘moral being’, all these conclusions will be applied to Assisted Human Reproduction, genetic cloning and stem cell research. Finally, under the light of these information, applicability of these techniques will be evaluated from cultural and faith tradition perspective.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Correlation Of Somatic CellSteroid Secretion And Quality OfGenerated Oocytes After In-VitroStimulation Of Mouse Follicles
250049
EN
Wang
W
Liu
H.Ch
He
Z
Rosenwaks
Z
Journal Article
2022
03
05
During folliculogenesis, follicular cells are crucial in initiating oocyte development and providing with nutrients and growth regulators for the oocytes. Somatic follicular cell differentiation is well coordinated with oocyte maturation. The objective of this project is to test if follicular cell steroidogenesis can be used as a marker for the quality of the embrace oocytes. We have developed an in-vitro culture system that supports the growth of preantral follicles and retains their competency for fertilization and subsequent embryo development in mouse models. Mechanically isolated mouse preantral follicles were cultivated singly in micro<font>drop</font>lets under oil in medium supplemented with recombinant FSH and LH at 37°C and 5% CO2.Under an optimal concentration of FSH and LH, these follicles underwent dramatic morphological changes, which ultimately led to the formation of antral follicles and the production of oocytes. At the initial stage of invitro culture (IVC)/maturation (IVM) of follicles, a high level of LH or FSH in the medium Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 34 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 facilitates E2 sevretion, enhances granulosa cell (GC) outgrowth, consequently leading to earlier antral formation. However, prolonged culture in high LH and FSH triggers early differentiation and luteinization of GCs, resulting in fewer metaphase II oocytes and blastocysts. Under the optimal concentration of LH (10mIU/ml) and FSH (100mIU/ml), follicular E2 production associated with matured oocytes was significantly higher than that of immature ones. Furthermore, matured follicles producing E2 with the range of 60- 80ng/ml produced oocytes of highest quality. Approximately 89% of MII oocytes showed an optimal level of E2 prior to ovulation and all of them were able to be fertilized and develop into blastocysts; wheras those oocytes producing a undesirable level of E2 degenerated progressively. In summary, active somatic cell steroidogenesis prior to ovulation and an ideal steroid milieu at ovulation are critical for the gereration of competent oocytes after follicular maturation invitro.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Evaluation Of ReproductivePotential After IntracytoplasmicSperm Injection Of Varied HumanSemen Tested By AntiacrosomalAntibodies
250803
EN
Tepla
O
Peknicova
L
Koci
K
Mika
J
Mrazek
M
Elzeinova
F
Journal Article
2022
03
06
<strong>Objective:</strong> To determine whether varied human spermatozoa detected also with monoclonal antibodies against acrosomal proteins, have an influence on fertilization, transfer, pregnancy and implantation rates using ICSI. Design: A retrospective study. Setting: A private IVF center and academic research laboratory. Patients: 1240 men participating in the ICSI program. Interventions: Sperm were divided into seven groups: oligozoospermia, oligoasthenozoospermia, ligoasthenoteratozoospermia, fresh and frozen/thawed epididymal and fresh and frozen/thawed testicular sperm. Fertilization, transfer, pregnancy and implantation rates were recorded in each category. Sperm were tested with antibodies for detection of the of the sperm acrosome. Main outcome measure: Fertilization, transfer, pregnancy and implantation rates, percentage of acrosome-reacted cells. Results: The fertilization rate and statistical evaluation showed differences between morphologically normal and pathological sperm and other groups. The freezing-thawing procedure had no influence on the fertilization of testicular sperm but epididymal frozen/thawed sperm had a higher fertilization rate. Immunofluorescence proved decreasing sperm quality in all groups compared to the control group. This difference is not manifested in other parameters (transfer, pregnancy, implantation rates). Conclusion(s): The spermatozoa with varied semen characteristics and good quality, also detected with specific antibodies, gave the best fertilization rates. The paternal effect is not proved in other parameters.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Trophoblast Cell Activationby Trophinin Ligation Is Implicatedin Human Embryo Implantation
250804
EN
Suguhara
K
Sugiyama
D
Kabir-Salmani
M
Byrne
J
Wolf
DP
Lowitz
KP
Kobayashi
Y
Nadano
D
Aoki
D
Nozawa
S
Nakayama
J
Mustelin
T
Ruoslahti
E
Yamaguchi
N
Fukuda
MN
Journal Article
2022
03
06
During human embryo implantation, trophectoderm mediates adhesion of the blastocyst to the uterine epithelium. The rapid growth of the embryo and invasion of the maternal tissue suggest adhesion-induced activation of the embryonal cells. We show here that ligation of trophinin, a homophilic cell adhesion molecule expressed on trophoblastic cells, induces tyrosine phosphorylation in trophinin-expressing trophoblastic HT-H cells. The phosphorylation could be induced in HT-H cells with the binding of trophinin-expressing cells or anti trophinin antibodies. Trophinin-dependent tyrosine phosphorylation was associated with actin reorganization. We also isolated trophinin-binding peptides from phage libraries. These peptides exhibited the consensus sequence GWRQ and seemed to reproduce the effects of trophinin-mediated cell adhesion. Upon binding of a GWRQ peptide, HT-H cells became highly proliferative and motile. HT-H cells expressed ErbB family receptors and bound EGF and heparin-binding EGF-like growth factor (HBEGF), but ErbB family receptor phosphorylation in these cells required Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 33 GWRQ. In the absence of GWRQ, trophinin interacted with the cytoplasmic protein bystin, which binds to ErbB4 and blocks its autophosphorylation. In HT-H cells, GWRQ peptide dissociated trophinin from bystin, and ErbB4 was activated. Culturing monkey blastocysts in the presence of the peptide increased total number and motility of the trophectoderm cells. These results suggest that trophinin-mediated cell adhesion functions as a molecular switch for trophectoderm activation in human embryo implantation.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Mouse Back Muscle As APromising Site For Human OvarianTissue Xenotransplantation
250805
EN
Soleimani
R
Heytens
E
Lierman
S
Cuvelier
C
Gerris
J
Dhont
M
Van Der Elst
J
De Sutter
P
Journal Article
2022
03
06
<strong>Introduction:</strong> Loss of follicles during initial ischemia constitutes the main limitation of ovarian tissue transplantation (OTT). We investigate the efficiency of the back muscle (B) site for human OTT using a SCID mouse xenografting model.
<strong>Materials and Methods:</strong> 1) Study of vascularization and integration of OT in the grafting site during the first eight days after transplantation and Immunohistochemical staining (Anti-human and anti-mouse CD31 and CD34) to evaluate neovascularization. 2) Follicular development in B and K xenografts, three, five and seven months after grafting.
<strong>Results: </strong>1) Anti-mouse CD34 and CD31 positive endothelial cells were first noticed in B and K grafts on days 3 and 4 respectively. On day 5 all B and 70% of K grafts and on day 8, all B and K grafts were positive for mouse markers, but a higher number of murine blood vessels were counted in the B versus K grafts (14.0±1.3 versus 6.60±0.68, p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
GSH Level, Spindle Area AndRate Of IVF In Presence OfAntioxidant In Mouse OocyteCultured In MEME Medium
250806
EN
Mohammadi
Roushandeh A
Pasbakhsh
P
Shokrgozar
M.A
Alizadeh
Z
Mahmoudi
R
Habibi Roudkenar
M
Journal Article
2022
03
06
<strong>Introduction:</strong> The study was carried out effects of different doses of cysteamine on rate of IVM and IVF. Also the study was carried out to study glutathione (GSH) synthesis in presence of cysteamine without cumulus cells in MEME medium. MII spindle area was analysed by immunocytochemistry for quantification of shape and size of it.
<strong>Materials and Methods:</strong> Female mice were primed with 5 IU of PMSG and GV oocytes were retrieved from the ovary 48 hr later for IVM. The IVM medium was supplemented with 0, 50, 100, 200 and 500 mM of cysteamine. Experiments also included a group of ovulated oocytes (in vivo matured) after priming with PMSG and HCG. Cytoplasmic GSH level was measured by DTNB-GR recycling protocol. For IVF MII oocytes were inseminated with mature mouse sperm and 24 hours after insemination rate of two cell embryo was measured. MII Oocytes were fixed and immunostained for microtubules, and chromosomes and then spindle area were analysed.
<strong>Results:</strong> After IVM, an improvement was observed on MII development in 200 μm cysteamine group. Intracytoplasmic GSH level increased in presence of cysteamine in 200μm cysteamine and Highest level of GSH was produced in In vivo. Spindle area in all in vitro groups exept 500μm increased and Spindle area in 200μm cysteamine compare to in vivo group was insignificant(p>0.05).
<strong>Conclusion: </strong>Our results showed that cysteamine improved IVM rate in dose dependant. Rate of two cell embryo increased significantly in 200μm cysteamine compare to control. Also cysteamine induced glutathione synthesis in MII oocyte and improved microtubule organization in 200μm cysteamine group.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Nuclear Donor Choice,Sperm-Mediated Activation AndEmbryo Aggregation: AMultipronged Approach ToSequentially Improve Cattle CloningEfficiency
250807
EN
Oback
B.J
Journal Article
2022
03
06
Cloning by somatic cell nuclear transfer (SCNT) remains very inefficient with only Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 31 about 1-5% of cloned blast cysts developing into viable offspring. In order to improve cloning efficiency, I first developed a zonafree NT procedure which doubles the throughput in cloned embryo and offspring production in cattle and mouse, increasing both ease of operation and reproducibility. I then used this method to determine at which step the NT procedure could be improved to increase cloning efficiency. I focused on the choice of nuclear donor cell type and cell cycle stage, the artificial activation method and cloned embryo culture conditions. Firstly, I hypothesized that cloning efficiency is inversely correlated with donor cell differentiation status and could be increased by using undifferentiated somatic stem cells as donors. By cloning the world,s first red deer from multipotent antler stem cells and their differentiated progeny, we showed that this was not the case. This finding was confirmed in cattle where myogenic cells of divergent differentiation status resulted in very similar cattle cloning efficiency, suggesting that cell plasticity and epigenetic reprogramming are biologically unrelated and somatic donor cell type is not critical for cloning success.we further demonstrated that, independent of donor cell type and cll line, cloning efficiency can be more than doubled by simple serum-starvation of donor cells. The next step towards improving cloning efficiency was using sperm rather than artificial means to activate cloned embryos. Implantation and birth of live offspring were significantly improved after sperm- mediated activation. Finally, we aggregated individual cloned embryos during in vitro culture to further increase cloning efficiency. In embryonic clones, aggregation led to a2-3 fold significant increase in survival to term. Somatic clones, however, showed compromised in vivo survival, revealing striking biological differences between embryonic and somatic clones in response to aggregation.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Neither Aurora B Activitynor Histone H3 Phosphorylation IsEssential For ChromosomeCondensation During MeioticMaturation Of Porcine Oocytes
250808
EN
Jelı Nkova
L
Kubelka
M
Journal Article
2022
03
06
Aurora kinase B (AURK B) is a chromosomal passenger protein that is essential for a number of processes during mitosis. Its activity is regulated by association with two other passenger proteins, INCENP and Survivin, and by phosphorylation on Thr 232. In this study, we have examined expression and phosphorylation (Thr-232) of Aurora kinase B during meiotic maturation of pig oocytes in correlation with histone H3 phosphorylation and chromosome condensation. We show that histone H3 phosphorylation on Ser-10, but not on Ser-28, correlates with progressive chromosome condensation during oocyte maturation; Ser- 10 phosphorylation starts around the time of the breakdown of the nuclear envelope, with the maximal activity in metaphase I, while Ser-28 phosphorylation does not significantly change in maturing oocytes. Treatment of oocytes with 50 uM Butyrolactone I (BL-I), an inhibitor of cyclindependent kinases, or cycloheximide (10ug/ml), inhibitor of proteosynthesis, results in a block of oocytes in the germinal vesicle (GV) stage, when nuclear membrane remains intact, however, condensed chromosome fibers or highly condensed chromosome bivalents can be seen in the nucleoplasm of BL-I- or cycloheximide-treated oocytes, respectively. In such treated oocytes no or only a very weak activity of Aurora kinase B, as well as phosphorylation of histone H3 on Ser-10 can be detected after 27 hours of treatment, whereas phosphorylation on Ser- 28 is not influenced. These results suggest that Aurora kinase B activity and Ser-10 phosphorylation of histone H3 are not required for chromosome condensation in pig oocytes, but might be required for further processing of chromosomes during meiosis.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Letrozole Effect On In VitroCulture Of Human EndometrialExplants In Fibrin Matrix
250809
EN
Khazaei
M
Montaseri
A
Ghorbani
R
Journal Article
2022
03
06
<strong>Introduction: </strong>In vitro culture of human endometrial tissue introduced as a model for endometriosis. Drug treatment options in endometriosis are unsatisfactory and more researches on this disease are needed. Aromatase inhibitors are candidate drug in the treatment of endometriosis. The aim of the present study is to investigate an aromatase inhibitor (letrozole) effects on human endometrium in three-dimensional (3D) fibrin matrix.
<strong>Materials and Methods:</strong> Normal human endometrial biopsies (N=8) from reproductive age women (mean age 28.3 year) were cultured in fibrin matrix for 21 days as a control and different doses of letrozole (0.1, 1 and 10 μM). Tissue changes were evaluated by two systems: an invert microscopic grading system (Marking criteria) and computerized program analysis. Routine histochemical and immunohistochemichal staining were done to document stromal, epithelial and endothelial cells. Data were analyzed by one way ANOVA and post hoc Tukey test.
<strong>Results: </strong>Letrozole (0.1, 1 and 10 μM) exert a significant (p
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
Clinical In Vitro Maturationof Human Oocytes
250810
EN
Ingolf Nielsen
H
Journal Article
2022
03
06
Oocyte maturation is generally defined as the reinitiation of the first meiotic division leading to metaphase II (MII), combined with the appropriate cytoplasmic processes, which are necessary for proper fertilization and early embryo development. Using in vitro maturation (IVM) techniques, oocytes are harvested in the GV stage (germinal vesicle, prophase I), and matured in special maturation media. Especially two major groups of patients may benefit from IVM: One group consists of regularly cycling women with normal ovaries referred for IVF and ICSI due to severe male infertility problems or tubal factor. The other group consists of women suffering from PCO/PCOS. These women are extremely sensitive to stimulation with follicle stimulating hormone (FSH) and have a significant risk of developing ovarian hyperstimulation syndrome (OHSS). An IVM expert meeting in Copenhagen in autumn 2006 came up with recommendations for the treatment of these two groups of Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 29 patients: Inclusion criteria: Age ≤ 35 years BMI 18 – 25 kg/m2 ≤ 3 previously failed IVF cycles ≤ 3 previous IVM attempts No endocrine abnormalities (e.g. hyperprolactinemia, hyperglycaemia, hyperthyroidism) No ovarian cyst>20 mm on cycle day 3 Estradiol level
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
History And Fundamentals OfOocyte Maturation In Vitr
250811
EN
Ingolf Nielsen
H
Journal Article
2022
03
06
Oocyte maturation is generally defined as the reinitiation of the first meiotic division leading to metaphase II (MII), combined with the appropriate cytoplasmic processes, which are necessary for proper fertilization and early embryo development. Using in vitro maturation techniques, oocytes are harvested in the GV stage (germinal vesicle, prophase I), and matured in special maturation media. Relevant aspects of oogenesis and follicular development will be described. In vitro maturation (IVM) of oocytes was first carried out in rabbits in 1935 by Pincus and Enzmann followed by Robert Edwards, who in 1965 did the first IVM on human oocytes. Ironically, the first IVM baby was the result of in vitro maturation of an immature oocyte from a stimulated cycle (Veeck 1983). Starting with Cha in 1989 the number of babies resulting from in vitro maturation of oocytes in unstimulated cycles has steadily increased. Several reasons for using IVM can be mentioned and will be discussed in this lecture, but especially two major groups of patients are interesting for this procedure: One group consists of regularly cycling women with normal ovaries referred for IVF and ICSI due to severe male infertility problems or tubal factor. The other group consists of women suffering from PCO/PCOS. These women are extremely sensitive to stimulation with follicle stimulating hormone (FSH) and have a significant risk of developing ovarian hyperstimulation syndrome (OHSS). Collection of immature oocytes combined with in vitro maturation may eliminate the risk of OHSS. The IVM techniques are still being developed and improved, and were most recently discussed at the Second International Symposium on In Vitro Maturation of Oocytes in connection with the ESHRE Meeting in Lyon, France, on July 1 2007. At this meeting an attempt was also made to produce an educated estimate of the number of IVM deliveries and ongoing pregnancies. This estimate ended up being around 1150 until the middle of 2007. It should be mentioned that more than half of those are from Asian countries.
Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)
Cell Journal (Yakhteh)
2228-5806
9
supplement1
2007
09
01
A Morphometric Study Onthe Endometrium Of Rat Uterus InHypothyroid And Thyroxine TreatedHypothyroid Rats
250812
EN
Inuwa
M.I
Williams
M.A
Journal Article
2022
03
06
Hypothyroidism increases the rate of pregnancy loss. Other manifestations include menstrual disorder, and infertility. Serum levels of gonadotropins are low in hypothyroid patients. Though studies of uterine ultrastructure are well established as approaches to investigating the pathophysiology of infertility, they have scarcely been extended to the study of hypothyroid related infertility. The present study investigates the effect of hypothyroidism on the ultrastructure of uterine epithelium. Three groups of Wistar rats were studied. Two groups were initially made hypothyroid using methimazole, and the third group was an untreated control. One hypothyroid group was given daily injections of thyroxine for six weeks. The uteri were removed in all three groups, and processed for transmission electron microscopy and morphometry. It was found that absolute epithelial cell volume was decreased in hypothyroidism. The volume of the nucleus had decreased though its relative volume in the cell had Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 28 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 increased. The height of the luminal epithelium in hypothyroid rats also decreased by (33.8%) as compared with controls. Basement membrane thickness was significantly increased in hypothyroidism. The changes were all substantially abrogated by the administration of thyroxine. This study suggests that thyroid hormones might be importantly concerned in the maintenance of the normal structure of uterine epithelial cells.