TY - JOUR ID - 253369 TI - A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Tanaka, Hiromitsu AU - Tokuhiro, Keizo AD - Labaratory of Molecular Biology, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, Japan AD - Department of Genome Editing, Institute of Biomedical Science, Kansai Medical University, Shin-machi, Hirakata City, Osaka, Japan Y1 - 2022 PY - 2022 VL - 24 IS - 9 SP - 552 EP - 554 KW - embryo KW - Haspin KW - inner cell mass KW - Germ Cell KW - Oocyte DO - 10.22074/cellj.2022.8444 N2 - HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element(hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studieshave shown that Haspin is also expressed in trace amounts in somatic cells; HASPIN also functions in oocytes.Haspin expression is regulated by the tissue-specific methylation of Haspin genomic DNA regions, including somaticcells. This study investigated relationship between 193PE and DNA methylation by examining methylation status oftransgenic mice carrying 193PE and a reporter gene. In somatic (liver) cells carrying the reporter gene, 193PE inducedmethylation as well as trace expression of the reporter gene. In the testis, 193PE induced hypomethylation and intensereporter gene expression. Expression of HASPIN in an egg was assessed using human chorionic gonadotrophin toinduce ovulation in female transgenic mice. The results showed that 193PE induced tissue-specific methylation, whichresulted in reporter gene expression in a mouse egg. UR - https://www.celljournal.org/article_253369.html L1 - https://www.celljournal.org/article_253369_5059429a9dcb8687172b9df0d42c792e.pdf ER -