TY - JOUR ID - 253308 TI - Alginate Effects on Human Sperm Parameters during Freezing and Thawing: A Prospective Study JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Feyzmanesh, Somayeh AU - Halvaei, Iman AU - Baheiraei, Nafiseh AD - Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Tissue Engineering and Applied Cell Sciences Division, Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2022 PY - 2022 VL - 24 IS - 7 SP - 417 EP - 423 KW - Alginate KW - encapsulation KW - Rapid Freezing KW - Sperm DNA DO - 10.22074/cellj.2022.8122 N2 - Objective: The main goal was to evaluate the effects of alginate on human sperm parameters during cryopreservation.Materials and Methods: In this prospective study, twenty-five normozoospermic samples were divided into two groups,encapsulated with 1% alginate and the control group. The samples were then frozen by rapid freezing. Different sperm parameters including motility, normal morphology, viability, acrosome reaction, and DNA integrity, were examined before freezing and after thawing.Results: All sperm parameters had a significant decrease after thawing compared to before freezing. Our data showed a significant decrease in sperm motility of the alginate group but sperm viability, normal morphology, and DNA fragmentation were similar between the two groups. However, the rates of intact acrosome and native DNA were significantly lower in the control group compared to the alginate group (45.12 ± 11.1 vs. 55.25 ± 10.69 and 52.2 ± 11.92vs. 68.12 ± 10.15, respectively, P<0.05).Conclusion: It seems that alginate can prevent premature acrosome reaction and protect sperm DNA from denaturation during the rapid freezing process. UR - https://www.celljournal.org/article_253308.html L1 - https://www.celljournal.org/article_253308_6aef2bcc824810e4f4e5877ea1f74d50.pdf ER -