TY - JOUR ID - 252433 TI - Improved Healing of Colonic Anastomosis with Allotransplantation of Axillary Skin Fibroblasts in Rats JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Sufian, Narges AU - Behfar, Mehdi AU - Tehrani, Ali-Asghar AU - Malekinejad, Hassan AD - Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AD - Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AD - Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran Y1 - 2022 PY - 2022 VL - 24 IS - 4 SP - 188 EP - 195 KW - Allogeneic Transplantation KW - Colorectal Surgery KW - Fibroblasts KW - Rats KW - Wound healing DO - 10.22074/cellj.2022.7861 N2 - Objective: Colonic anastomosis is associated with serious complications leading to significant morbidity and mortality. Fibroblasts have recently been introduced as a practical alternative to stem cells because of their differentiation capacity, anti-inflammatory, and regenerative properties. The aim of this study was to evaluate the effects of intramural injection of fibroblasts on the healing of colonic anastomosis in rats. Materials and Methods: Inbred mature male Wistar rats were used in this experimental study (n=36). Fibroblasts were isolated from the axillary skin of a donor rat. In the sham group, manipulation on descending colon was done during laparotomy. A 5 mm segment of the colon was resected, and end-to-end anastomosis was performed. In the control group, 0.5 ml of phosphate buffer saline (PBS) was injected into the colonic wall and in the treatment group, 1×106 fibroblasts were transplanted. Following euthanasia on day 7, intra-abdominal adhesion, leakage and peritonitis were evaluated by necropsy. Mechanical properties were assessed using bursting pressure and tensile tests. Inflammation, angiogenesis, and collagen deposition were examined histopathologically.Results: The mean scores for adhesion and leakage were decreased in the treatment group versus control samples. Lower infiltration of inflammatory cells was observed in the treatment group (P=0.03). Angiogenesis and collagen deposition scores were significantly increased in the fibroblast transplanted group (P=0.03). Tensile mechanical properties of the colon were significantly increased in the treatment group compared to the control sample (P=0.01). There was no significant difference between the control and treatment groups in terms of bursting pressure (P=0.10). Positive weight changes were found in sham and treatment groups, but the control rats lost weight after 7 days. Conclusion: The results suggested that allotransplantation of dermal fibroblasts could improve the necroscopic,  histopathological, and biomechanical indices of colonic anastomosis repair in rats. UR - https://www.celljournal.org/article_252433.html L1 - https://www.celljournal.org/article_252433_cb823573a1c3a6c73485b67b618b385a.pdf ER -