TY - JOUR ID - 252422 TI - CYP19A1 Promoters Activity in Human Granulosa Cells: A Comparison between PCOS and Normal Subjects JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Hashemain, Zohreh AU - Amiri-Yekta, Amir AU - Khosravifar, Mona AU - Alvandian, Faezeh AU - Shahhosseini, Maryam AU - Hosseinkhani, Saman AU - Afsharian, Parvaneh AD - Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran AD - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran AD - Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran Y1 - 2022 PY - 2022 VL - 24 IS - 4 SP - 170 EP - 175 KW - Aromatase KW - Granulosa KW - luciferase KW - Polycystic Ovary Syndrome KW - Promoter DO - 10.22074/cellj.2022.7787 N2 - Objective: Estrogen, a female hormone maintaining several critical functions in women's physiology, e.g., folliculogenesis and fertility, is predominantly produced by ovarian granulosa cells where aromatase enzyme converts androgen to estrogen. The principal enzyme responsible for this catalytic reaction is encoded by the CYP19A1 gene, with a long  regulatory region. Abnormalities in this process cause metabolic disorders in women, one of the most common of which is polycystic ovary syndrome (PCOS). The main purpose of this research was to determine the effect of the promoters on aromatase expression in cells with normal and PCOS characteristics. Materials and Methods: In this experimental study, four promoters of the CYP19A1 gene, including PII, I.3, I.4, and PII/ I .3 promoter fragments, were cloned upstream of the luciferase gene and transfected into normal and PCOS granulosa cells. Subsequently, the effect of follicle-stimulating hormone (FSH) on the activity of these regulatory regions was examined in the presence and absence of FSH. Western blotting was used to confirm aromatase expression in all groups. Data analysis was performed using ANOVA and paired sample t test, compared by post-hoc least significant difference (LSD) test. Results: Luciferase results confirmed the intense activity of PII promoter in the presence of FSH. Moreover, the study demonstrated reduced activity of PII promoter in normal granulosa cells, possibly due to the regulatory region of I.3 next to PII. Conclusion: FSH stimulates transcription of aromatase enzyme by affecting PII promoter, a process regulated by the inhibitory role of the I.3 region in PII activity in granulosa cells. Given the distinct role of these promoters in normal and PCOS granulosa cells, the importance of nuclear factors residing in these regions can be discerned.  UR - https://www.celljournal.org/article_252422.html L1 - https://www.celljournal.org/article_252422_f1050c51373899183b92690e65114ca2.pdf ER -