TY - JOUR ID - 251914 TI - The Impact Of Different Cell Culture Mediums On CD8+T Cells Expansion: A Bioinformatics Study JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Jalili, Arsalan AU - Hajifathali, Abbas AU - Bereimipour, Ahmad AU - Roshandel, Elham AU - Aghdami, Nasser AD - Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran AD - Hematopoeitic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran AD - Department of Regenerative Biomedicine and Cell Terapy, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Y1 - 2022 PY - 2022 VL - 24 IS - 3 SP - 155 EP - 162 KW - Expansion KW - Microarray KW - TCD8+ Cells DO - 10.22074/cellj.2022.7779 N2 - Objective: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signalingpathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select thebest candidate.Materials and Methods: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis.Results: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosomedynamics, telomeres, telomerase, and cellular aging signal pathways.Conclusion: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media.  UR - https://www.celljournal.org/article_251914.html L1 - https://www.celljournal.org/article_251914_c3cec0c91ea8193394ef61a494b272ab.pdf ER -