TY - JOUR ID - 251897 TI - Circ_0000228 Promotes Cervical Cancer Progression Via Regulating MiR-337-3p/TGFBR1 Axis JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Xu, Yongqian AU - Dong, Xiaona AU - Ma, Baoli AU - Mu, Pingping AU - Kong, Xiang AU - Li, Dongmei AD - Department of Gynecology and Obstetrics, Shengli Oilfield Central Hospital, Dongying, Shandong, China. AD - Department of Gynecology and Obstetrics, Shengli Oilfield Central Hospital, Dongying, Shandong, China Y1 - 2022 PY - 2022 VL - 24 IS - 2 SP - 91 EP - 98 KW - cervical cancer KW - miR-337-3p KW - TGFBR1 DO - 10.22074/cellj.2022.7914 N2 - Objective: This study aims to investigate the biological function of circular RNA (circRNA) circ_0000228 in the cervicalcancer (CC).Materials and Methods: In this experimental study, the GSE113696 dataset was downloaded from the Gene ExpressionOmnibus (GEO). GEO2R was employed to obtain differentially expressed circRNA between CC tissues and matched paracancerous tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were employed to detect circ_0000228, microRNA-337-3p (miR-337-3p) and transforming growth factor, beta receptor I (TGFBR1) expression levels in the CC tissues and cells. Following gain-of-function and loss-of-function models establishment, CCK8 and BrdU tests were conducted to examine cell proliferation. Transwell experiment was executed to examine CC cells migration and invasion. A lung metastasis model was utilized to determine the ability of circ_0000228 on the lung metastasis. Bioinformatics analysis, dual-luciferase reporter experiment and RNA immunoprecipitation (RIP) assay were applied to verify the targeting relationship among miR-337-3p, circ_0000228, and TGFBR1.Results: Circ_0000228 expression in the CC tissues and cells was up-modulated. Circ_0000228 overexpression markedly enhanced cell proliferation, migration, and invasion, while knocking down circ_0000228 remarkably repressed cell proliferation, migration, and invasion. MiR-337-3p could be adsorbed by circ_0000228. TGFBR1 was identified as a target gene of miR-337-3p that indirectly and positively modulated by circ_0000228 in the CC cells. Conclusion: Circ_0000228 up-modulates TGFBR1 by targeting miR-337-3p to enhance CC cell proliferation, migrationand invasion. Also, Circ_0000228 is a promising therapeutic target for the CC. UR - https://www.celljournal.org/article_251897.html L1 - https://www.celljournal.org/article_251897_dae3a70aa88a8f1ca21c4c2c03fb3569.pdf ER -