TY - JOUR ID - 251707 TI - Direct Differentiation Of Human Primary Fibroblast Into Hematopoietic-Like Stem Cells; A New Way Without Viral Transduction JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Habibi, Sina AU - Khamisipour, Gholamreza Khamisipour AU - Obeidi, Narges AU - Zare Jaliseh, Saeedeh AD - Department of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, Iran AD - Department of Hematology, Faculty of Allied Medicine, Bushehr University of Medical Sciences, Bushehr, Iran AD - Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2020 PY - 2020 VL - 22 IS - supplement 1 SP - 141 EP - 147 KW - Direct Differentiation KW - Electromagnetic waves KW - Fibroblast KW - Hematopoietic Stem Cells KW - Reprogramming DO - 10.22074/cellj.2020.6846 N2 - Objective: The aim of this study was to investigate the possibility of producing safe hematopoietic stem cells without the use of viral infectious agents that can be used in stem cell transplantation.Materials and Methods: In this experimental study, after single layer cell formation, human primary fibroblast cells weretreated with static electromagnetic fields of 10 and 15 milli Tesla (mT) for 20 minutes each day for seven consecutive days. On the seventh day and immediately after the last radiation, the cells were added to the wells containing specific hematopoietic stem cell expansion media. After 21 days and colony formation, the cells belonging to each group wereevaluated in terms of the expression of CD34, CD38, and GATA-1 genes using quantitative real-time polymerase chain reaction (PCR), as well as surface marker expression of CD34 by flow cytometry.Results: Exposure to 10 mT and 15 mT electromagnetic field increased the expression of CD34 and CD38 genes (P<0.05). This increase in gene expression levels were 2.85 and 1.84 folds, respectively, in the 10mT group and 6.36 and 3.81 folds, respectively, in the 15 mT group. The expression of the GATA-1 gene in the 10 mT and 15 mT groups was not significantly different from that of the control group (P<0.05). Electromagnetic waves caused a marked increase in the expression of the CD34 marker at the surface of reprogrammed cells. The rate of expression was about 42.3% in the 15 mT group and 23.1% in the 10 mT group.Conclusion: The presence of human primary fibroblasts exposed to electromagnetic fields can increase the expressionof specific hematopoietic genes. This method can be suitable for reprogramming cells differentiated into hematopoieticlike stem cells and does not pose the risks of retroviral use. UR - https://www.celljournal.org/article_251707.html L1 - https://www.celljournal.org/article_251707_936077f3f1cbe88a5d123d0d62528aa6.pdf ER -