TY - JOUR ID - 250726 TI - Identification of CCNB2 as A Potential Non-Invasive Breast Cancer Biomarker in Peripheral Blood Mononuclear Cells Using The Systems Biology Approach JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Moradpoor, Raheleh AU - Zali, Hakimeh AU - Gharebaghian, Ahmad AU - Akbari, Mohammad Esmaeil AU - Ajdari, Soheila AU - Salimi, Mona AD - . Department of Basic Sciences, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - .School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences,Tehran, Iran AD - .Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - 4.Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - 5.Department of Immunology, Pasteur Institute of Iran, Tehran, Iran AD - 6.Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran Y1 - 2021 PY - 2021 VL - 23 IS - 4 SP - 406 EP - 413 KW - biomarker KW - Breast cancer KW - Peripheral Blood Mononuclear Cell KW - Systems biology DO - 10.22074/cellj.2021.7053 N2 - ObjectiveBreast cancer (BC) still remains an imperative clinical issue, despite advances in the diagnosis, prognosis and treatment modalities of this malignancy. Hence, progress has been made to identify non-invasive, high sensitive and specific biomarkers. Since immune system affects development of breast cancer, peripheral blood mononuclear cells (PBMCs) -a subpopulation of immune cells- can be considered as a promising tool in the field of BC biomarker research. In the current study, we initially attempted to use concept of the present shared biomarkers in solid tumors and systemic immune profile and then evaluate correlation of these biomarkers to clinical use in cancer research. Materials and MethodsIn this experimental study, available microarray gene expression datasets of BC as well as the related PBMCs were retrieved and downloaded from the Gene Expression Omnibus (GEO) database, followed by analysis using GEO2R along with affylmGUI, a R-based package, to obtain differentially expressed genes (DEGs). Signature genes from 20 types of cancer were also applied to validate DEGs. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was carried out to assess mRNA level of CCNB2 in PBMC of the BC patients and healthy subjects. ResultsDEGs analysis for the transcription profile of BC cells and PBMCs showed two shared targets, CCNB2 and PGK1. Validation with systems biology using reweighted 20 types of cancer signature genes revealed that CCNB2 is the only common target in BC and its related PBMCs, which was further validated by qRT-PCR implying a significant increase in the level of CCNB2 in the BC patients. ConclusionResults of this study demonstrated that PBMCs are affected by BC cells and CCNB2 may be of value as a diagnostic biomarker for breast cancer. However, verification would require future detailed experimental plans. UR - https://www.celljournal.org/article_250726.html L1 - https://www.celljournal.org/article_250726_810114af1345d18a00ad7ccc5579d155.pdf ER -