TY - JOUR ID - 250711 TI - An Integrative Analysis of The Micro-RNAs Contributing in Stemness, Metastasis and B-Raf Pathways in Malignant Melanoma and Melanoma Stem Cell JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Sahranavardfard, Parisa AU - Madjd, Zahra AU - Emami Razavi, Amirnader AU - Ghanadan, Alireza AU - Firouzi, Javad AU - Khosravani, Pardis AU - Ghavami, Saeid AD - .Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran AD - .Department of Pathology, Iran University of Medical Sciences, Tehran, Iran AD - .Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran AD - .Iran National Tumor Bank, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran ;4.Department of Dermatopathology, Razi Skin Hospital, Tehran University of Medical Sciences, Tehran, Iran AD - 5.Department of Human Anatomy and Cell Sciences, University of Manitoba, Manitoba, Canada ;6.Biology of Breathing, Children Hospital Research Institute of Manitoba, University of Manitoba, Winnipeg, Canada ;7.Autophagy Research Ce Y1 - 2021 PY - 2021 VL - 23 IS - 3 SP - 261 EP - 272 KW - Epithelial KW - Mesenchymal Transition KW - Melanoma KW - MicroRNA KW - Network Analysis DO - 10.22074/cellj.2021.7311 N2 - ObjectiveEpithelial-mesenchymal transition (EMT) and the stemness potency in association with BRAF mutation are in dispensable to the progression of melanoma. Recently, microRNAs (miRNAs) have been introduced as the regulator of a multitude of oncogenic functions in most of tumors. Therefore identifying and interpreting the expression patterns of these miRNAs is essential. The present study sought to find common miRNAs regulating all three important pathways in melanoma development. Materials and MethodsIn this experimental study, 18 miRNAs that importantly contribute to EMT and have a role in regulating self-renewal and the BRAF pathway were selected based on current literature and cross-analysis with available databases. Subsequently, their expression patterns were evaluated in 20 melanoma patients, normal tissues, serum from patients and control subjects, and melanospheres. Pattern discovery and integrative regulatory network analysis were used to find the most important miRNAs in melanoma progression. ResultsAmong 18 selected miRNAs, miR-205, -141, -203, -15b, and -9 were differentially expressed in tumor samples than normal tissues. Among them, miR-205, -15b, and -9 significantly expressed in serum samples and healthy donors. Attribute Weighting and decision trees (DT) analysis presented evidence that the combination of miR-205, -203, -9, and -15b can regulate self-renewal and EMT process, by affecting CDH1, CCND1, and VEGF expression. ConclusionWe suggested here that miR-205, -15b, -203, -9 pattern as the key miRNAs linked to melanoma status, the pluripotency, proliferation, and motility of malignant cells. However, further investigations are required to find the mechanisms underlying the combinatory effects of the above mentioned miRNAs. UR - https://www.celljournal.org/article_250711.html L1 - https://www.celljournal.org/article_250711_941d625a8140b43da1abfe42f0099395.pdf ER -