TY - JOUR ID - 250693 TI - Long-Term Effects of Hippocampal Low-Frequency Stimulation on Pro-Inflammatory Factors and Astrocytes Activity in Kindled Rats JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Rohani, Razieh AU - Aliaghaei, Abbas AU - Abdollahifar, Mohammad-Amin AU - Sadeghi, Yousef AU - Zare, Leila AU - Dehghan, Samaneh AU - Heidari, Mohammad Hassan AD - .Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences and Health Services (SBMU), Tehran, Iran AD - .Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Y1 - 2021 PY - 2021 VL - 23 IS - 1 SP - 85 EP - 92 KW - Deep Brain Stimulation KW - Epilepsy KW - GFAP KW - Interleukin KW - 6 KW - TNF KW - α DO - 10.22074/cellj.2021.7139 N2 - ObjectiveEpilepsy is accompanied by inflammation, and the anti-inflammatory agents may have anti-seizure effects. In this investigation, the effect of deep brain stimulation, as a potential therapeutic approach in epileptic patients, was investigated on seizure-induced inflammatory factors. Materials and Methods In the present experimental study, rats were kindled by chronic administration of pentylenetetrazol (PTZ; 34 mg/Kg). The animals were divided into intact, sham, low-frequency deep brain stimulation (LFS), kindled, and kindled +LFS groups. In kindled+LFS and LFS groups, animals received four trains of intra-hippocampal low-frequency deep brain stimulation (LFS) at 20 minutes, 6, 24, and 30 hours after the last PTZ injection. Each train of LFS contained 200 pulses at 1 Hz, 200 µA, and 0.1 ms pulse width. One week after the last PTZ injection, the Y-maze test was run, and then the rats’ brains were removed, and hippocampal samples were extracted for molecular assessments. The gene expression of two pro-inflammatory factors [interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α)], and glial fibrillary acidic protein (GFAP) immunoreactivity (as a biological marker of astrocytes reactivation) were evaluated. Results Obtained results showed a significant increase in the expression of of interleukin-6 (IL-6), tumor necrosis factor (TNF)-α, and GFAP at one-week post kindling seizures. The application of LFS had a long-lasting effect and restored all of the measured changes toward normal values. These effects were gone along with the LFS improving the effect on working memory in kindled animals. Conclusion The anti-inflammatory action of LFS may have a role in its long-lasting improving effects on seizure-induced cognitive disorders. UR - https://www.celljournal.org/article_250693.html L1 - https://www.celljournal.org/article_250693_a6b87c0bfed415a6ec495682d8f2a152.pdf ER -