TY - JOUR ID - 250679 TI - The Effect of Composol Medium on miR-16 Expression during Platelet Storage up to Day 7 at Room Temperature JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Rajabi, Ali AU - Sharifi, Zohreh AU - Yari, Fatemeh AU - Deyhim, Mohammadreza AU - Jalili, Mohammadali AD - Y1 - 2020 PY - 2020 VL - 22 IS - 4 SP - 542 EP - 547 KW - Blood Platelets KW - MicroRNAs KW - MiR KW - 16 KW - Platelet Storage DO - 10.22074/cellj.2021.6790 N2 - ObjectiveMicroRNAs (miRNAs) are short, noncoding RNAs that play vital roles in gene regulation. It has been shown that storage has an effect on platelet miRNAs. MiR-16 is highly expressed in platelets and it appears to target the genes involved in cell death. It has been shown that platelets could be stored in Composol for a longer period of time. The aim of the present study was to assess and compare the expression pattern of miR-16 in platelet concentrates (PCs) in plasma and Composol media. Materials and Methods In an experimental study, ten PC bags were collected and each bag was divided into two separate bags, one with the 70% Composol and the other with only plasma. Both bags were stored for 7 days at 22˚C and tested on days 1, 3, 5, and 7 of storage. For each sample, we performed quantitative real-time polymerase chain reaction (qRT-PCR). The water-soluble tetrazolium salt-1 (WST-1) test was used to assess platelet viability in all of the samples. Statistical analysis was done by SPSS and REST software. A P < 0.05 was considered statistically significant. Results miR-16 was significantly elevated during the storage days, with fold changes of 3.47 (plasma) and 2.77 (Composol). The Composol group had significantly decreased miR-16 expression compared with the plasma group. Results of the WST-1 test showed less decrease in optical density (OD) in the Composol group (0.93 ± 0.4) during the storage days compared with the plasma group (0.75 ± 0.3). Conclusion Our finding supported results from previous studies that reported an increase in miR-16 expression during platelet storage. In addition, miR-16 down-regulation in Composol medium implied that Composol might be a good solution for long-term platelet storage because it has the potential to elevate the shelf-life of platelets stored at 22˚C. UR - https://www.celljournal.org/article_250679.html L1 - https://www.celljournal.org/article_250679_0c2c61ff78b0967e76a43ab086edbc7d.pdf ER -