TY - JOUR ID - 250632 TI - Designing A Transgenic Chicken: Applying New Approaches toward A Promising Bioreactor JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Bahrami, Salahadin AU - Amiri-Yekta, Amir AU - Daneshipour, Abbas AU - Jazayeri, Seyedeh Hoda AU - Mozdziak, Paul Edward AU - Sanati, Mohammad Hossein AD - .Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran AD - .Physiology Graduate Program, North Carolina State University, Raleigh, NC, USA AD - .Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;.Department of Medical Genetics, National Institute of Genetic Engineering and Biotechn Y1 - 2019 PY - 2019 VL - 22 IS - 2 SP - 133 EP - 139 KW - Chickens KW - CRISPR/CAS9 KW - Ovalbumin KW - Recombinant protein KW - Transgenes DO - 10.22074/cellj.2020.6738 N2 - Specific developmental characteristics of the chicken make it an attractive model for the generation of transgenic organisms. Chicken possess a strong potential for recombinant protein production and can be used as a powerful bioreactor to produce pharmaceutical and nutritional proteins. Several transgenic chickens have been generated during the last two decades via viral and non-viral transfection. Culturing chicken primordial germ cells (PGCs) and their ability for germline transmission ushered in a new stage in this regard. With the advent of CRISPR/Cas9 system, a new phase of studies for manipulating genomes has begun. It is feasible to integrate a desired gene in a predetermined position of the genome using CRISPR/Cas9 system. In this review, we discuss the new approaches and technologies that can be applied to generate a transgenic chicken with regards to recombinant protein productions. UR - https://www.celljournal.org/article_250632.html L1 - https://www.celljournal.org/article_250632_28bcbbfa5eff5857783cd4591dd4c664.pdf ER -