TY - JOUR ID - 250588 TI - Autologous Bone Marrow Stem Cell Transplantation in Liver Cirrhosis after Correcting Nutritional Anomalies, A Controlled Clinical Study JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Esmaeilzadeh, Abbas AU - Ommati, Homeira AU - Kooshyar, Mohammad Mahdi AU - Jarahi, Lida AU - Akhavan Rezayat, Kambiz AU - Saberi, Samaneh AU - Vosough, Massoud AU - Ghassemi, Ali AD - Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Gastroenterology and Hepatology Research Center, Mashhad, Iran AD - Department of Hematology and Oncology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - 4HPGC Group, Medical Biotechnology Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran AD - 5Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;6Department of Stem Cells and Developmental Biology, Cell Science Research AD - 7Department of Pediatric Hematology and Oncology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2019 PY - 2019 VL - 21 IS - 3 SP - 268 EP - 273 KW - Bone marrow stem cells KW - Cell therapy KW - Cirrhosis KW - Regenerative medicine DO - 10.22074/cellj.2019.6108 N2 - ObjectiveLiver transplantation is the gold standard approach for decompensated liver cirrhosis. In recent years, stem cell therapy has raised hopes that adjusting some clinical and laboratory parameters could lead to successful treatments for this disease. Cirrhotic patients may have multiple systemic abnormalities in peripheral blood and irregular cell populations in bone marrow (BM). Correcting these abnormalities before BM aspiration may improve the effectiveness of cell-based therapy of liver cirrhosis. Materials and MethodsIn this controlled clinical trial study, 20 patients with decompensated liver cirrhosis were enrolled. Patients were randomly assigned to control and experimental groups. Blood samples were obtained to measure vitamin B12, folate, serum iron, total iron bonding capacity (TIBC) and ferritin before any intervention. Furthermore, the iron storage and fibrosis level in BM biopsies, as well as the percentage of different cell populations, were evaluated. Prior to cell isolation for transplantation, we performed palliative supplement therapy followed by a correction of nutritional deficiencies. Mononuclear cells (MNCs) were then isolated from BM aspirates and transfused through peripheral vein in patients in the experimental group. The model of end-stage liver disease (MELD) score, The international normalized ratio (INR), serum albumin and bilirubin levels were assessed at 0 (baseline), 3 and 6 months after cell transplantation. ResultsThe MELD score (P=0.0001), INR (P=0.012), bilirubin (P < 0.0001) and total albumin (P < 0.0001) levels improved significantly in the experimental group after cell transplantation compared to the baseline and control groups. Moreover, the increase in serum albumin levels of patients in the experimental group was statistically significant 6 months after transplantation. ConclusionWe have successfully improved the conditions of preparing -BM-derived stem cells for transplantation. Although these cells are relatively safe and have been shown to improve some clinical signs and symptoms temporarily, there need to be more basic studies regarding the preparation steps for effective clinical use (Registration number: IRCT2014091919217N1). UR - https://www.celljournal.org/article_250588.html L1 - https://www.celljournal.org/article_250588_00c9b0111e4fb4e7b1fc101d5f6ea69a.pdf ER -