TY - JOUR ID - 250523 TI - Low Frequency Electrical Stimulation Attenuated The Epileptiform Activity-Induced Changes in Action Potential Features in Hippocampal CA1 Pyramidal Neurons JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Ghasemi, Zahra AU - Naderi, Nima AU - Shojaei, Amir AU - Ahmadirad, Nooshin AU - Raoufy, Mohammad Reza AU - Mirnajafi-Zadeh, Javad AD - Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran AD - Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran Y1 - 2018 PY - 2018 VL - 20 IS - 3 SP - 355 EP - 360 KW - Action potential KW - Brain stimulation KW - Hippocampus KW - Rat DO - 10.22074/cellj.2018.5443 N2 - ObjectiveElectrical low frequency stimulation (LFS) is a new therapeutic method that moderates hyperexcitability during epileptic states. Seizure occurrence is accompanied by some changes in action potential (AP) features. In this study, we investigated the inhibitory action of LFS on epileptiform activity (EA) induced-changes in AP features in hippocampal CA1 pyramidal neurons. Materials and Methods In this experimental study, we induced EA in hippocampal slices by increasing the extracellular potassium (K+) concentration to 12 mM. LFS (1 Hz) was applied to the Schaffer collaterals at different pulse numbers (600 and 900) at the beginning of the EA. Changes in AP features recorded by whole-cell patch clamp recording were compared using phase plot analysis. Results Induction of EA depolarized membrane potential, decreased peak amplitude, as well as the maximum rise and decay slopes of APs. Administration of 1 Hz LFS at the beginning of EA prevented the above mentioned changes in AP features. This suppressive effect of LFS depended on the LFS pulse number, such that application of 900 pulses of LFS had a stronger recovery effect on AP features that changed during EA compared to 600 pulses of LFS. The constructed phase plots of APs revealed that LFS at 900 pulses significantly decreased the changes in resting membrane potential (RMP), peak amplitude, and maximum rise and decay slopes that appeared during EA. Conclusion Increasing the numbers of LFS pulses can magnify its inhibitory effects on EA-induced changes in AP features. UR - https://www.celljournal.org/article_250523.html L1 - https://www.celljournal.org/article_250523_014c9cf550ca78f94e461d5bd858a408.pdf ER -