TY - JOUR ID - 250491 TI - Evaluating The Effect of Melatonin on HAS2 , and PGR Expression, as Well as Cumulus Expansion, and Fertility Potential in Mice JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Ezzati, Maryam AU - Roshangar, Leila AU - Soleimani Rad, Jafar AU - Karimian, Nahid AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Tissue Engineering, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran;Department of Tissue Engineering, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AD - 4Department of Advanced Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Y1 - 2018 PY - 2018 VL - 20 IS - 1 SP - 108 EP - 112 KW - Hyaluronan Synthase KW - 2 KW - Melatonin KW - Mouse Oocyte KW - Progesterone Receptor DO - 10.22074/cellj.2018.4894 N2 - ObjectiveInfertility is a worldwide health problem which affects approximately 15% of sexually active couples. One of the factors influencing the fertility is melatonin. Also, protection of oocytes and embryos from oxidative stress inducing chemicals in the culture medium is important. The aim of the present study was to investigate if melatonin could regulate hyaluronan synthase-2 (HAS2 ) and Progesterone receptor (PGR ) expressions in the cumulus cells of mice oocytes and provide an in vitro fertilization (IVF) approach. Materials and Methods In this experimental study, for this purpose, 30 adult female mice and 15 adult male mice were used. The female mice were superovulated using 10 U of pregnant mare serum gonadotropin (PMSG) and 24 hours later, 10 U of human chorionic gonadotropin (hCG) were injected. Next, cumulus oocyte complexes (COCs) were collected from the oviducts of the female mice by using a matrix-flushing method. The cumulus cells were cultured with melatonin 10 µM for 6 hours and for real-time reverse transcription-polymerase chain reaction (RT-PCR) was used for evaluation of HAS2 and PGR expression levels. The fertilization rate was evaluated through IVF. All the data were analyzed using a t test. Results The results of this study showed that HAS2 and PGR expressions in the cumulus cells of the mice receiving melatonin increased in comparison to the control groups. Also, IVF results revealed an enhancement in fertilization rate in the experimental groups compared to the control groups. Conclusion To improve the oocyte quality and provide new approaches for infertility treatment, administration of melatonin as an antioxidant, showed promising results. Thus, it is concluded that fertility outcomes can be improved by melatonin it enhances PGR . UR - https://www.celljournal.org/article_250491.html L1 - https://www.celljournal.org/article_250491_a1fe0cf70a791e93aef39bcb03e293c1.pdf ER -