TY - JOUR ID - 250469 TI - Effects of Re-Vitrification of Mouse Morula and Early Blastocyst Stages on Apoptotic Gene Expression and Developmental Potential JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Majidi Gharenaz, Nasrin AU - Movahedin, Mansoureh AU - Mazaheri, Zohreh AD - Y1 - 2018 PY - 2018 VL - 19 IS - 4 SP - 614 EP - 619 KW - Apoptosis KW - embryo KW - genes KW - Mouse DO - 10.22074/cellj.2018.4892 N2 - ObjectiveRe-vitrification of embryos immediately after thawing or after a culture period could be used to preserve the extra embryos after embryo transfer. This study aims to clarify the effect of re-vitrification on Bax and Bcl-2 gene expressions of compact and early blastocyst stage embryos. Materials and Methods This experimental study was performed on mouse embryos. We collected 8-cell stage embryos (n=400) from female mature mice, 60-62 hoursafter injection of human chorionic gonadotropin (hCG). The embryos were divided into 5 groups: fresh (n=80), vitrified at the 8-cell stage (n=80), vitrified at the blastocyst stage (n=80), vitrified at the 8-cell stage, and re-vitrified at the compact (n=80) and early blastocyst stages (n=80). Embryos were vitrified by cryolock. We analyzed the developmental rates of the vitrified-warmed embryos with the chi-square test. Quantitative polymerase chain reaction (qPCR) data were analyzed with SPSS version 16 using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. P < 0.05 were considered statistically significant. Results The expanded blastocyst formation rate showed a significant difference in re-vitrified embryos compared with fresh embryos (P < 0.05). However, this result was similar between the two re-vitrified groups. Our data showed a significant difference in expression of the Bax and Bcl-2 genes between re-vitrified and fresh embryos (P < 0.05). Expressions of the Bax and Bcl-2 genes showed no significant difference between the two re-vitrified groups. Conclusion Based on our study, re-vitrification could affect developmental rate and expressions of the Bax and Bcl2 genes. UR - https://www.celljournal.org/article_250469.html L1 - https://www.celljournal.org/article_250469_c150727923d2d31cd491e6ced526b3e8.pdf ER -