TY - JOUR ID - 250435 TI - Methoxyamine Enhances 5-Fluorouracil-Induced Radiosensitization in Colon Cancer Cell Line HT29 JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Khoei, Samideh AU - Poorabdollahi, Roghayeh AU - Mostaar, Ahmad AU - Faeghi, Fariborz AD - Razi Drug Research Centre, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran AD - Department of Radiology Technology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - 4Department of Medical Physics and Medical Engineering, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran Y1 - 2017 PY - 2017 VL - 19 IS - 2 SP - 283 EP - 291 KW - 5 KW - Fluorouracil KW - Methoxyamine KW - Radiation KW - Comet assay KW - Colon cancer DO - 10.22074/cellj.2016.4295 N2 - ObjectiveThis study intended to observe the effects of methoxyamine (Mx) on cytotoxic effects and DNA damage caused by 5-Fluorouracil (5-FU) in combination with gamma radiation in a human colon cancer cell line, HT29. Materials and Methods In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Next, the cells were irradiated with 2 Gy gamma radiation. After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays. Results Cytotoxicity and DNA damage increased with increasing 5-FU concentration. The 1 mM Mx concentration had no significant effect on cytotoxicity and DNA damage from 5-FU; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-FU when used in combination with 2 Gy gamma radiation. Conclusion Mx combined with 5-FU enhanced the radiosensitivity of colon cancer cells. UR - https://www.celljournal.org/article_250435.html L1 - https://www.celljournal.org/article_250435_2b6493f67a779832020bcac592fea4e3.pdf ER -