TY - JOUR ID - 250374 TI - Mesenchymal Stromal Cells Implantation in Combination with Platelet Lysate Product Is Safe for Reconstruction of Human Long Bone Nonunion JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Labibzadeh, Narges AU - Emadedin, Mohsen AU - Fazeli, Roghayeh AU - Mohseni, Fatemeh AU - Hosseini, Seyedeh Esmat AU - Moghadasali, Reza AU - Mardpour, Soura AU - Azimian, Vajiheh AU - Ghorbani Liastani, Maede AU - Mirazimi Bafghi, Ali AU - Baghaban Eslaminejad, Mohamadreza AU - Aghdami, Nasser AD - Y1 - 2016 PY - 2016 VL - 18 IS - 3 SP - 302 EP - 309 KW - Fractures Ununited KW - Mesenchymal Stromal Cells KW - Platelet Lysate DO - 10.22074/cellj.2016.4557 N2 - ObjectiveNonunion is defined as a minimum of 9 months since injury without any visible progressive signs of healing for 3 months. Recent literature has shown that the application of mesenchymal stromal cells is safe, in vitro and in vivo, for treating long bone nonunion. The present study was performed to investigate the safety of mesenchymal stromal cell (MSC) implantation in combination with platelet lysate (PL) product for treating human long bone nonunion. Materials and MethodsIn this case series clinical trial, orthopedic surgeons visited eighteen patients with long bone nonunion, of whom 7 complied with the eligibility criteria. These patients received mesenchymal stromal cells (20 million cells implanted once into the nonunion site using a fluoroscopic guide) in combination with PL product. For evaluation of the effects of this intervention all the patients were followed up by taking anterior-posterior and lateral X-rays of the affected limb before and 1, 3, 6, and 12 months after the implantation. All side effects (local or systemic, serious or non-serious, related or unrelated) were observed during this time period. ResultsFrom a safety perspective the MSC implantation in combination with PL was very well tolerated during the 12 months of the trial. Four patients were healed; based on the control Xray evidence, bony union had occurred. ConclusionResults from the present study suggest that the implantation of bone marrow-derived MSCs in combination with PL is safe for the treatment of nonunion. A double blind, controlled clinical trial is required to assess the efficacy of this treatment (Registration Number: NCT01206179). UR - https://www.celljournal.org/article_250374.html L1 - https://www.celljournal.org/article_250374_99b21c068e09ad3e6a572fe683fe6539.pdf ER -