TY - JOUR ID - 250349 TI - Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Saylan, Aslihan AU - Duman, Selcuk AD - Y1 - 2016 PY - 2016 VL - 18 IS - 1 SP - 83 EP - 88 KW - Annexin V KW - Apoptosis KW - Hyaluronic acid KW - human DO - 10.22074/cellj.2016.3990 N2 - ObjectiveIn 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. Materials and MethodsThis observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. Human serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups annexin V was used to determine the rate of apoptosis of these sperm. ResultsFollowing swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher apoptosis rate in the HSA group compared to the HA group. ConclusionThe addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility. UR - https://www.celljournal.org/article_250349.html L1 - https://www.celljournal.org/article_250349_3a8bc55687ee9410672a20231ff8fdef.pdf ER -