TY - JOUR ID - 250306 TI - Expression of COLLAGEN 1 and ELASTIN Genes in Mitral Valvular Interstitial Cells within Microfiber Reinforced Hydrogel JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Eslami, Maryam AU - Javadi, Gholamreza AU - Agdami, Nasser AU - Shokrgozar, Mohammad Ali AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;Department of Genetics,Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran;Applied Biotechnology Research Center, Tehran M AD - Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran AD - 4Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran AD - 5Cell Bank Division, Pasteur Institute of Iran (IPI), Tehran, Iran Y1 - 2015 PY - 2015 VL - 17 IS - 3 SP - 478 EP - 488 KW - Tissue engineering KW - Heart valve KW - ELASTIN KW - COLLAGEN I KW - Real KW - Time PCR DO - 10.22074/cellj.2015.22 N2 - ObjectiveThe incidence of heart valve disease is increasing worldwide and the number of heart valve replacements is expected to increase in the future. By mimicking the main tissue structures and properties of heart valve, tissue engineering offers new options for the replacements. Applying an appropriate scaffold in fabricating tissue-engineered heart valves (TEHVs) is of importance since it affects the secretion of the main extracellular matrix (ECM) components, collagen 1 and elastin, which are crucial in providing the proper mechanical properties of TEHVs. Materials and MethodsUsing real-time polymerase chain reaction (PCR) in this experi- mental study, the relative expression levels of COLLAGEN 1 and ELASTIN were obtained for three samples of each examined sheep mitral valvular interstitial cells (MVICs)-seeded onto electrospun poly (glycerol sebacate) (PGS)-poly (ε-caprolactone) (PCL) microfibrous, gelatin and hyaluronic acid based hydrogel-only and composite (PGS-PCL/hydrogel) scaffolds. This composite has been shown to create a synthetic three-dimensional (3D) microenvironment with appropriate mechanical and biological properties for MVICs. ResultsCell viability and metabolic activity were similar among all scaffold types. Our results showed that the level of relative expression of COLLAGEN 1 and ELASTIN genes was higher in the encapsulated composite scaffolds compared to PGS-PCL-only and hydrogel-only scaffolds with the difference being statistically significant (P < 0.05). ConclusionThe encapsulated composite scaffolds are more conducive to ECM secretion over the PGS-PCL-only and hydrogel-only scaffolds. This composite scaffold can serve as a model scaffold for heart valve tissue engineering. UR - https://www.celljournal.org/article_250306.html L1 - https://www.celljournal.org/article_250306_773792f39b3983b27142bdbb7eb2b40d.pdf ER -