TY - JOUR ID - 250269 TI - Evaluation of mRNA Contents of YBX2 and JHDM2A Genes on Testicular Tissues of Azoospermic Men with Different Classes of Spermatogenesis JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Najafipour, Reza AU - Moghbelinejad, Sahar AU - Samimi Hashjin, Amir AU - Rajaei, Farzad AU - Rashvand, Zahra AD - Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran;Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran AD - Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran AD - Department of Medical Genetics, Qazvin University of Medical Sciences, Qazvin, Iran Y1 - 2015 PY - 2015 VL - 17 IS - 1 SP - 121 EP - 128 KW - Azoospermia DO - 10.22074/cellj.2015.518 N2 - ObjectiveAnimal model studies have shown that MSY2 and JHDM2A genes have an important role in spermatogenesis process and fertility of male mice. But the potential role of these genes in human spermatogenesis and fertility is not known yet. Therefore, we evaluated expression ratios of these genes in testis tissues of men with normal and impaired spermatogenesis. Materials and MethodsIn this experimental study, after RNA extraction and cDNA syn- thesis from 50 non-obstructive azoospermic and 12 normal testis tissues, the expression ratios of genes were evaluated by real time polymerase chain reaction (PCR) technique. Hematoxcylin and eosin (H&E) staining was used for histological classification of testis tissues. For statistical analysis, one way analysis of variance (ANOVA) test was carried out. ResultsOur results showed a significant reduction in mRNA level of YBX2 in samples with impaired spermatogenesis (p < 0.001) compared to samples with qualitatively normal spermatogenesis and normal spermatogenesis; however, in JHDM2A gene, despite sensible reduction in gene expression level in men with impaired spermatogenesis, no significant differences were shown (p > 0.05). Furthermore in YBX2, a significant negative correlation was demonstrated between the efficiency score of spermatogenesis and the threshold cycle (CT) (r=-0.7, p < 0.0001), whereas in JHDM2A, this negative correlation was not significant (r=-0.4, p=0.06). ConclusionGenerally, these data indicated that YBX2 and JHDM2A genes may play an important role in male infertility, and suggested that these molecules can act as useful biomarkers for predicting male infertility. UR - https://www.celljournal.org/article_250269.html L1 - https://www.celljournal.org/article_250269_a720765dd01f3f7cefe0aa45590098d6.pdf ER -