TY - JOUR ID - 250268 TI - Radioprotective Effect of Melatonin on Radiation-Induced Lung Injury and Lipid Peroxidation in Rats JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Tahamtan, Raziyeh AU - Shabestani Monfared, Ali AU - Tahamtani, Yasser AU - Tavassoli, Alireza AU - Akmali, Maasoomeh AU - Mosleh-Shirazi, Mohammad Amin AU - Naghizadeh, Mohammad Mehdi AU - Ghasemi, Danial AU - Keshavarz, Mojtaba AU - Haddadi, Gholam Hassan AD - Department of Biophysics and Biochemistry, Cellular and Molecular Biology Research Centre, Babol University of Medical Sciences, Babol, Iran AD - Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran AD - Department of Pathology, Fasa University of Medical Sciences, Fasa, Iran AD - 4Department of Biochemistry, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - 5Department of Radiotherapy, Physics Unit, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran AD - 6Department of Biostatistics, Fasa University of Medical Sciences, Fasa, Iran AD - 7Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran AD - 8Young Researchers and Elite Club, Shiraz Branch, Islamic Azad University of Shiraz, Shiraz Iran AD - 9Department of Medical Physics, Fasa University of Medical Sciences, Fasa, Iran Y1 - 2015 PY - 2015 VL - 17 IS - 1 SP - 111 EP - 120 KW - Radiation KW - Melatonin KW - Pulmonary fibrosis DO - 10.22074/cellj.2015.517 N2 - ObjectiveFree radicals generated by ionizing radiation attack various cellular components such as lipids. The lung is a very radiosensitive organ and its damage is a doselimiting factor in radiotherapy treatments. Melatonin (MLT), the major product of the pineal gland acts as a radioprotective agent. This study aims to investigate the radioprotective effects of MLT on malondialdehyde (MDA) levels and histopathological changes in irradiated lungs. Materials and MethodsIn this experimental study, a total of 62 rats were divided into five groups. Group 1 received no MLT and radiation (unT), group 2 received oral MLT (oM), group 3 received oral MLT and their thoracic areas were irradiated with 18 Gy (oMR), group 4 received MLT by intraperitoneal (i.p.) injection and their thoracic areas were irradiated with 18 Gy (ipM-R), group 5 received only 18 Gy radiation in the thoracic area (R). Following radiotherapy, half of the animals in each group were sacrificed at 48 hours for evaluation of lipid peroxidation and early phase lung injuries. Other animals were sacrificed in the eighth week of the experiment for evaluation of the presence of late phase radiation induced lung injuries. ResultsPre-treatment of rats with either i.p injection (p < 0.05) and oral administration of MLT (p < 0.001) significantly reduced MDA levels in red blood cell (RBC) samples compared to the R group. Furthermore, i.p. injection of MLT decreased MDA levels in plasma and tissue (p < 0.05). In the early phase of lung injury, both administration of MLT significantly increased lymphocyte (p < 0.05) and macrophage frequency (p < 0.001). MLT reduced the lung injury index in the lungs compared to the R group (p < 0.05). ConclusionThe result of this study confirms the radioprotective effect of MLT on lipid peroxidation, and in both early and late phases of radiation induced lung injuries in an animal model. UR - https://www.celljournal.org/article_250268.html L1 - https://www.celljournal.org/article_250268_86b978470258d6e00198cb433e6b7e76.pdf ER -