TY - JOUR ID - 250240 TI - Differentiation Potential of O Bombay Human-Induced Pluripotent Stem Cells and Human Embryonic Stem Cells into Fetal Erythroid-Like Cells JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Ganji, Fatemeh AU - Abroun, Saeid AU - Baharvand, Hossein AU - Aghdami, Nasser AU - Ebrahimi, Marzieh AD - Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran AD - Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;Department of Hematology, School of Medical Sciences, Tarbiat AD - Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;Department of Regenerative Biomedicine at Cell Science Resear Y1 - 2015 PY - 2015 VL - 16 IS - 4 SP - 426 EP - 439 KW - Induced Pluripotent Stem Cells KW - Differentiation KW - Hemangioblasts KW - Erythroid Cells DO - 10.22074/cellj.2015.489 N2 - ObjectiveThere is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells (hiPSCs) have provided a valuable alternative source to differentiate into any desired cell type as a therapeutic promise to cure many human disease. Materials and MethodsIn this experimental study, we examined the erythroid differentiation potential of normal Bombay hiPSCs (B-hiPSCs) and compared results to human embryonic stem cell (hESC) lines. Because of lacking ABO blood group expression in B-hiPSCs, it has been highlighted as a valuable source to produce any cell type in vitro. ResultsSimilar to hESC lines, hemangioblasts derived from B-hiPSCs expressed approximately 9% KDR+CD31+ and approximately 5% CD31+CD34+. In semisolid media, iPSC and hESC-derived hemangioblast formed mixed type of hematopoietic colony. In mixed colonies, erythroid progenitors were capable to express CD71+GPA+HbF+ and accompanied by endothelial cells differentiation. ConclusionFinally, iPS and ES cells have been directly induced to erythropoiesis without hemangioblast formation that produced CD71+HbF+ erythroid cells. Although we observed some variations in the efficiency of hematopoietic differentiation between iPSC and ES cells, the pattern of differentiation was similar among all three tested lines. UR - https://www.celljournal.org/article_250240.html L1 - https://www.celljournal.org/article_250240_0aa8babaa21996edec0e0ada146e706d.pdf ER -