TY - JOUR ID - 250211 TI - The Effects of Exendine-4 on Insulin Producing Cell Differentiation from Rat Bone Marrow-Derived Mesenchymal Stem Cells JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Nejad-Dehbashi, Fereshteh AU - Hashemitabar, Mahmoud AU - Orazizadeh, Mahmoud AU - Bahramzadeh, Somaieh AU - Shahhosseini Pourshoushtary, Elham AU - Khorsandi, Layasadat AD - Cell and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran AD - Cell and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran;Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Y1 - 2014 PY - 2014 VL - 16 IS - 2 SP - 187 EP - 194 KW - Exendin KW - 4 KW - Mesenchymal stem cells KW - Insulin KW - producing Cells KW - PDX KW - 1 KW - GLUT KW - 2 DO - N2 - ObjectiveThe aim of this study was to evaluate the effect of exendin-4 (EX-4) on differentiation of insulin-producing cells (IPCs) from rat bone marrow-derived mesenchymal stem cells (RAT-BM-MSCs). Materials and Methods In this experimental study, RAT-BM-MSCs were cultured and the cells characterized by flow cytometry analysis of cell surface markers. RAT-BM-MSCs were subsequently treated with induction media with or without EX-4. After induction, the presence of IPCs was demonstrated with dithizone (DTZ) staining and gene expression profiles for pancreatic cell differentiation markers (PDX-1, GLUT-2, insulin) were assessed using reverse transcription polymerase chain reaction (RT-PCR). Insulin excreted from differentiated cells was analyzed with radioimmunoassay (RIA). The two-tailed student’s t-test was used for comparison of the obtained values. Results The percentage of DTZ-positive cells significantly increased in EX-4 treated cells (p < 0.05). Expression of the islet-associated genes PDX-1, GLUT-2 and insulin genes in EX-4 treated cells was markedly higher than in the cells exposed to differentiation media without EX-4. RIA analysis demonstrated significant release of insulin with the glucose challenge test in EX-4 treated cells compared to EX-4 untreated cells. Conclusion The results of this study have demonstrated that EX-4 can enhance differentiation of IPCs from RAT-BM-MSCs. UR - https://www.celljournal.org/article_250211.html L1 - https://www.celljournal.org/article_250211_0c4943ddb524512bd2b69e73cadf69c7.pdf ER -