TY - JOUR ID - 248808 TI - Detection Of Nitric Oxide Reduction During Ischaemia-Reperfusion By EPR Spectroscopy JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Kadkhodaei, M. AU - Zhang, B. AU - Hanson, G.R. AU - Willgoss, D.A. AU - Gobe, G.C. AU - Endre, Z.H. AD - PHYSIOLOGY DEPARTMENT, TEHRAN MEDICAL SCIENCES UNIVERSITY, TEHRAN, IRAN AD - Y1 - 2001 PY - 2001 VL - 3 IS - 3 SP - 137 EP - 142 KW - Nitric Oxide KW - ELECTRON PARAMAGNETIC RESONANCE KW - Kidney KW - ISCHAEMIA. REPERFUSION DO - N2 - Introduction: Acute renal failure is a common consequence of sepisis due to concurrent renal ischaemia. The role of nitric oxide (NO) in endotoxaemia and in ischaemic injury in the kidney is not well defined.Material and Methods: In this study we have used an animal model of sepsis induced by injection of bacterial lipopolysaccharide (LPS) in the rat and measured renal nitric oxide by X-band electron paramagnetic resonance (EPR) spectroscopy using the spin trap Fe2+-N-methyl-D-glucamine dithiocarbamate [Fe(MGD)2] given by intravenous injection 6 minutes before sacrifice.Results: The characteristic EPR spectrum of [Fe(NO)(MGD)2] was observed in kidneys of rats treated with LPS for 5h. Rat kidneys subjected to 20 min ischaemia and 5 min reperfusion had lower concentrations of [Fe(NO)(MGD)2] (1.0 ± 0.6 (M) compared to the contralateral nonischaemic kidneys (1.5 ± 0.9 (M, P<0.05).Conclusion: This study shows reduced levels of NO after renal ischaemia in vivo. UR - https://www.celljournal.org/article_248808.html L1 - https://www.celljournal.org/article_248808_43735fcfdc3482aac56fd7e54667533e.pdf ER -