TY - JOUR ID - 248447 TI - Effect Of Β-Mercaptoethanol With And Without BSO (DL-Buthionine Sulfoximine) On Resumption Of Meiosis, In Vitro Maturation And Embryo Development Of Immature Mouse Oocytes JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Eimani, Hossein AU - Hassani, Fatemeh AU - Haerie-Rohani, Seyed Ali AU - Nasr Esfahani, Mohammad Hossein AU - Dalman, Azam AU - Rezazadeh Valojerdi, Mojtaba AU - Ashtiani, Saeed Kazemi AU - Shahverdi, Abdol Hossein AU - Baharvand, Hossein AU - Eftekhari Yazdi, Poopak AU - Samani, Reza Omani AD - Anatomy Department, Faculty of Medicine, Baghiyatallah University - Embryology Department, Royan Institute, Tehran, Iran AD - Y1 - 2006 PY - 2006 VL - 7 IS - 4 SP - 236 EP - 241 KW - IVM KW - Mouse KW - Oocyte KW - BSO KW - Β-MERCAPTOETHANOL DO - N2 - Introduction: The purpose of this study was to evaluated the effect of β-mercaptoethanol on resumption of meiosis, in vitro maturation of immature mouse oocytes and resulting embryo development with and without BSO (DL-Buthionine sulfoximine).Material and Methods: Germinal vasicle (GV) were recovered from 6-8 weeks old NMRI ovaries and cultured in maturation medium in MEMα supplemented with 7.5IU/ml hCG, 100mIU/ml rhFSH, 5% FCS (control group) and adding 100μm β-mercaptoethanol (group 1) or with 5mM BSO + 100μm β-mercaptoethanol (group 2) for 24h. The matured oocytes then were fertilized and cultured for 5 days. Fertilization and development were accomplished in T6 medium.Results: The percentage of GV oocytes reaching to metaphase I (or undergo GVBD) were 78.5%, 85%, 86% in control group, group 1 and group 2 respectively, that no significant difference was detected between groups. The proportion of oocytes that progressed to the metaphase II (MII) stage was minimum within 5mM BSO group (group 2) and maximum within β-mercaptoetanol group (group 1) with significant difference comparing with control and each other (P≤0.05). The percentage of embryos reaching to morula stage within β-mercaptoetanol group was significantly higher than the control group (5% and 12.2% respectively). None of oocytes treated with BSO could pass the 8 cell stage.Conclusion: β-mercaptoetanol enhances IVM and improves embryo development. While adding BSO into the maturation medium even with β-mercaptoetanol decreases maturation and declines the embryo development. UR - https://www.celljournal.org/article_248447.html L1 - https://www.celljournal.org/article_248447_28d9a0d4b0ab8fad639525a87f9136f6.pdf ER -