TY - JOUR ID - 248235 TI - Differentiation Of Murine Embryonic Stem Cells Into Endothelial Cells JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Fathi, F. AU - Jafari Kermani, A. AU - Mowla, SJ. AU - Poladi, A. AD - Anatomy Department, Cellular and Molecular Research Center, Kurdistan University of Medical Sciences AD - Genetic Department, Tarbiat Modaress University Y1 - 2008 PY - 2008 VL - 9 IS - 4 SP - 262 EP - 267 KW - Embryonic stem cells KW - Endothelial cells KW - EGM-2 KW - Differentiation DO - N2 - Objective: In this investigation Murine Embryonic Stem (ES) cells were differentiated into endothelial cells.Materials and Methods: Murine ES cells (CCE cell line) exposed to Alpha-MEM medium containing 10% FBS for 4 days. Then obtained Flk-1 (Flk-1: Vascular Endothelial Growth Factor Receptor 2) Positive cells were cultuted in Endothelial Growth Medium-2 (EGM-2) until the last day of experiment. Differentiated cells were evaluated by immunocytochemistry, RT-PCR and Tube Formation Assays.Results: When the ES cells cultured in collagen coated dishes containing Alpha-MEM & FBS, Flk-1 positive cells were obtained. After transfering Flk-1 positive cells into fibronectin coated dishes containing EGM2, the cells were assumed a relatively uniform endothelial cell morphology and could be propagated and expanded. Immunocytochemical and RT-PCR analysis of differentiated cells showed that they take up acetylated low-density lipoprotein (LDL), express Flk-1, CD31 and bind the BS-l lectin. When placed in Matrigel, these Murine ES cell–derived endothelial cells formed capillary-like structures characteristic of endothelial cellsConclusion: ES cell–derived endothelial cells provide a novel means to examine the mechanisms of endothelial cell development, and may open up new therapeutic strategies. UR - https://www.celljournal.org/article_248235.html L1 - https://www.celljournal.org/article_248235_9ac167e95898971e8272b7a03cae9315.pdf ER -