%0 Journal Article %T Effects of Different Perfusing Routes through The Portal Vein, Hepatic Vein, and Biliary Duct on Whole Rat Liver Decellularization %J Cell Journal (Yakhteh) %I Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR) %Z 2228-5806 %A Jambar Nooshin, Bahram %A Tayebi, Tahereh %A Babajani, Amirhesam %A Khani, Mohammad Mehdi %A Niknejad, Hassan %D 2023 %\ 01/01/2023 %V 25 %N 1 %P 35-44 %! Effects of Different Perfusing Routes through The Portal Vein, Hepatic Vein, and Biliary Duct on Whole Rat Liver Decellularization %K Biliary duct %K Decellularization %K Hepatic vein %K Portal vein %K Tissue engineering %R 10.22074/cellj.2022.557600.1081 %X Objective:Organ transplantation is the last therapeutic choice for end-stage liver failure, which is limited by the lack ofsufficient donors. Decellularized liver can be used as a suitable matrix for liver tissue engineering with clinical applicationpotential. Optimizing the decellularization procedure would obtain a biological matrix with completely removed cellularcomponents and preserved 3-dimensional structure. This study aimed to evaluate the decellularization efficacy throughthree anatomical routes.Materials and Methods:In this experimental study, rat liver decellularization was performed through biliary duct (BD),portal vein (PV), and hepatic vein (HV); using chemical detergents and enzymes. The decellularization efficacy wasevaluated by measurement of DNA content, extracellular matrix (ECM) total proteins, and glycosaminoglycans (GAGs).ECM preservation was examined by histological and immunohistochemical (IHC) staining and scanning electronmicroscopy (SEM). Scaffold biocompatibility was tested by the MTT assay for HepG2 and HUVEC cell lines.Results:Decellularization through HV and PV resulted in a transparent scaffold by complete cell removal, while the BDroute produced an opaque scaffold with incomplete decellularization. H&E staining confirmed these results. MaximumDNA loss was obtained using 1% and 0.5% sodium dodecyl sulfate (SDS) in the PV and HV groups and the DNAcontent decreased faster in the HV group. At the final stages, the proteins excreted in the HV and PV groups weresignificantly less than the BD group. The GAGs level was diminished after decellularization, especially in the PV andHV groups. In the HV and PV groups the collagen amount was significantly more than the BD group. The IHC and SEMimages showed that the ECM structure was preserved and cellular components were entirely removed. MTT assayshowed the biocompatibility of the decellularized scaffold.Conclusion:The results revealed that the HV is a more suitable route for liver decellularization than the PV and BD. %U https://www.celljournal.org/article_255056_df7a2c0906f7ae5d24667c3719403cac.pdf