%0 Journal Article %T Integrative Analysis Of LncRNAs In Kidney Cancer To Discover A New LncRNA (LINC00847) As A Therapeutic Target For Staphylococcal Enterotoxin Tst Gene %J Cell Journal (Yakhteh) %I Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR) %Z 2228-5806 %A Safarpour-Dehkordi, Maryam %A Doosti, Abbas %A Jami, Mohammad-Saied %D 2020 %\ 10/01/2020 %V 22 %N supplement 1 %P 101-109 %! Integrative Analysis Of LncRNAs In Kidney Cancer To Discover A New LncRNA (LINC00847) As A Therapeutic Target For Staphylococcal Enterotoxin Tst Gene %K Apoptosis %K Long Non-Coding RNA %K MicroRNA %K mRNA %K TSST-1 Toxin %R 10.22074/cellj.2020.6996 %X Objective: Bacterial toxin can cause cell death through induction of apoptosis in cancer cell lines as well as changes in the expression patterns of long non-coding RNAs (lncRNAs) and genes. In the present study, the effect of tst gene on ACHN cell lines was reported along with proposing a novel pathway of apoptosis in kidney cancer.Materials and Methods: In this experimental study, effective lncRNAs and genes were predicted from different criteriafor renal cell carcinoma (RCC) by bioinformatics methods and lncRNA-miRNA-mRNA interaction was constructed; then the effect of Staphylococcus aureus tst gene on induction of apoptosis pathways on ACHN and HDF cell lines was investigated.Results: After creation of lncRNA-miRNA-mRNA interaction, changes in expression levels of lncRNA LINC00847 (P=0.0024) and PTEN gene (P=0.0027) were identified, as potential apoptosis biomarkers for kidney cancer, after treating ACHN cell line by pCDNA3.1 (+)-tst compared to the empty vector. In contrast, there was no statistically significant difference in DICER1 expression levels in ACHN-tst cell (P≥0.05). In addition, transfection by pcDNA3.1 (+)-tst could increase ACHN cell apoptosis level (P<0.0001) compared to the pcDNA3.1 (+) group; but no significant effect was observed on normal cells.Conclusion: It is suggested that lncRNA LINC00847, discovered in this study, could provide a new landscape for researches aimed to determine relationship between functional lncRNA and RCC pathways. pcDNA3.1 (+)-tst wasfound to increase apoptosis in the transfected cells. %U https://www.celljournal.org/article_251702_e06dd593b48d94caa95da65886ae074a.pdf