%0 Journal Article %T Rapid Diagnosis Of Neisseria Meningitides By PCR Method %J Cell Journal (Yakhteh) %I Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR) %Z 2228-5806 %A Ataee, R.A. %A Ali Zadegan, M. Gorban %A Hajia, M. %A Tavana, A. Mehrabi %A Godarzi, Z. %A Nakhjavani, F. %D 2006 %\ 07/01/2006 %V 8 %N 2 %P 92-97 %! Rapid Diagnosis Of Neisseria Meningitides By PCR Method %K Meningitis %K NEISSERIA MENINGITIDIS %K PCR %K ctrA Gene %R %X Introduction: The aim of this study was to setup, optimize and introduce a sensitive and specific PCR detection method for identification of Neisseria meningitidis DNA in clinical samples.Material and Methods: Capsular transport gene A (ctrA) was selected as a specific target sequence. This primer pair amplifies 101bp of the target gene. Neisseria meningitidis strain: ATCC; 13090 and Neisseria meningitides serogroup C were used as a standard organism for optimization experiments. A range of bacterial pathogens were used for specificity testing, including, Haemophilus influenzae Type b: ATCC; 49766, Escherichia coli: ATCC;35218, Enterobacter, Klebsiella pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus group D. Phenol – chloroform method was used for DNA extraction. Amplified product was detected by gel agarose electrophoresis, stained by ethidiome bromide.Results: Our results confirmed amplification of the expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg of Neisseria meningitidis DNA as a final detection limit.Conclusion: As a conclusion, PCR is a method with high sensivity and specificity and specificity which can be performed within 3 hours, and therefore utilization of this test in the clinical laboratories can help rapid diagnosis of Neisseria meningitides in clinical samples. %U https://www.celljournal.org/article_248426_db5997174a56bb917fc12f94c429a614.pdf