@article { author = {Jomehpour, Mahshid and Khosravi, Mohammadmahdi and Janfada, Mehrnaz and Abroun, Saeid and Vahdat, Sadaf}, title = {Establishment of A Three-Dimensional Culture Condition for The U266 Cell Line Based on Peripheral Blood Plasma-Derived Fibrin Gels}, journal = {Cell Journal (Yakhteh)}, volume = {25}, number = {4}, pages = {229-237}, year = {2023}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2023.562849.1138}, abstract = {Objective: The study of pathophysiology as well as cellular and molecular aspects of diseases, especially cancer,requires appropriate disease models. In vitro three-dimensional (3D) structures attracted more attention to recapitulatediseases rather than in vitro two-dimensional (2D) cell culture conditions because they generated more similarphysiological and structural properties. Accordingly, in the case of multiple myeloma (MM), the generation of 3Dstructures has attracted a lot of attention. However, the availability and cost of most of these structures can restrict theiruse. Therefore, in this study, we aimed to generate an affordable and suitable 3D culture condition for the U266 MMcell line.Materials and Methods: In this experimental study, peripheral blood-derived plasma was used to generate fibrin gelsfor the culture of U266 cells. Moreover, different factors affecting the formation and stability of gels were evaluated.Furthermore, the proliferation rate and cell distribution of cultured U266 cells in fibrin gels were assessed.Results: The optimal calcium chloride and tranexamic acid concentrations were 1 mg/ml and 5 mg/ml for gel formationand stability, respectively. Moreover, the usage of frozen plasma samples did not significantly affect gel formation andstability, which makes it possible to generate reproducible and available culture conditions. Furthermore, U266 cellscould distribute and proliferate inside the gel.Conclusion: This available and simple fibrin gel-based 3D structure can be used for the culture of U266 MM cells in acondition similar to the disease microenvironment.}, keywords = {Blood plasma,Fibrin,Multiple Myeloma,Three-Dimensional Culture}, url = {https://www.celljournal.org/article_701713.html}, eprint = {https://www.celljournal.org/article_701713_769ebeb15abd88b639ab2eee05b2887b.pdf} }