@article { author = {Chaichian, Shahla and Mehdizadeh Kashi, Abolfazl and Tehermanesh, kobra and Pirhajati Mahabadi, Vahid and Minaeian, Sara and Eslahi, Neda}, title = {Effect of PLGA Nanoparticle-Mediated Delivery of miRNA 503 on The Apoptosis of Ovarian Endometriosis Cells}, journal = {Cell Journal (Yakhteh)}, volume = {24}, number = {11}, pages = {697-704}, year = {2022}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2022.557554.1069}, abstract = {Objective:One of the challenges in gene therapy is the transfer of the gene to the target cell. MicroRNAs (miRNAs)regulate gene expression after transcription by binding directly to the messenger and play a vital role in cell behaviorsand the pathogenesis of some diseases. This study was aimed at developing poly (lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) for gene delivery to endometriotic cyst stromal cells (ECSCs).Materials and Methods:In this experimental study, endometriosis cells were isolated from women with severeendometriosis (DIE) and digested by the enzymatic method (40 μg/ml DNAase I and 300 μg/ml collagenase type 3).PLGA-based NPs were synthesized and characterized. The size of sole PLGA NPs and PLGA/miRNA were 60 ± 4 nmand 70 ± 5.1 nm respectively. Poly lactic-co-glycolic-based NPs were used as vector carriers for miRNA 503 transfectionin endometriosis cells. The cells were divided into the five groups of control and four doses (25, 50, 75, and 100 μm) ofmiRNA 503/PLGA at 12, 24, 48, and 72 hours. Viability and apoptosis were evaluated by the MTT assay and AnnexinKits. Data were analyzed by one-way analysis of variance.Results:The results show that the size of PLGA/miRNA complex with dynamic light scattering (DLS) was 70 ± 5.1nm and zeta potential values of the PLGA/PEI/miRNA complexes were 27.9 mV. Based on the MTT assay results,the optimal dose of miRNA 503/PLGA was 75 μm, at which the viability of ECSCs was 52.6% ± 1.2 (P≤0.001), andthe optimal time was 48 hours. The apoptotic rates of ECSCs treated with PLGA/miRNA503 (34.75 ± 4.9%) weresignificantly higher than those of ECSCs treated with PLGA alone (3.35 ± 2.58%, P≤0.01).Conclusion:Cell death increased with increasing the concentration of miRNA; thus, it can be suggested as a treatmentfor endometriosis.}, keywords = {Apoptosis,miRNA 503,Nanoparticle,Ovarian Endometriosis}, url = {https://www.celljournal.org/article_254346.html}, eprint = {https://www.celljournal.org/article_254346_f1457366327588f3683d9724488e570d.pdf} }