@article { author = {Pirani, Hossein and Bakhtiari, Ali and Amiri, Bahareh and Salehi, Omid Reza}, title = {Beneficial Mitochondrial Biogenesis in Gastrocnemius Muscle Promoted by High-Intensity Interval Training in Elderly Female Rats}, journal = {Cell Journal (Yakhteh)}, volume = {25}, number = {1}, pages = {11-16}, year = {2023}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2022.557565.1078}, abstract = {Objective:Exercise can attenuate mitochondrial dysfunction caused by aging. Our study aimed to compare 12weeks of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the expressionof mitochondria proteins [e.g., AMP-activated protein kinase (AMPK), Estrogen-related receptor alpha (ERRα), p38mitogen-activated protein kinase (P38MAPK), and Peroxisome proliferator-activated receptor gamma coactivator1-alpha (PGC1-α)] in gastrocnemius muscle of old female rats.Materials and Methods:In this experimental study, thirty six old female Wistar rats (18-month-old and 270-310 g) weredivided into three groups: i. HIIT, ii. MICT, and iii. Control group (C). The HIIT protocol was performed for 12 weeks with16-28 minutes (2 minutes training with 85-90% VO2max in high intensity and 2 minutes training with 45-75% VO2max low intensity). The MICT was performed for 30-60 minutes with the intensity of 65-70% VO2max. The gastrocnemius muscle expression of AMPK, ERRα, P38MAPK, and PGC1α proteins were determined by Western blotting.Results:The expression of AMPK (P=0.004), P38MAPK (P=0.003), PGC-1α (P=0.028), and ERRα (P=0.006) in HIITwas higher than C group. AMPK (P=0.03), P38MAPK (P=0.032), PGC-1α (P=0.015), and ERRα (P=0.028) in MICTwas higher than the C group. Also expression of AMPK (P=0.008), P38MAPK (P=0.009), PGC-1α (P=0.020) and ERRα(P=0.014) in MICT was higher than MICT group.Conclusion:It seems that exercise training has beneficial effects on mitochondrial biogenesis, but the HIIT trainingmethod is more effective than MICT in improving mitochondrial function in aging.}, keywords = {Aging,Exercise,Mitochondrial,Muscle}, url = {https://www.celljournal.org/article_254301.html}, eprint = {https://www.celljournal.org/article_254301_e5227b983a6cd283d37f53a2d6a413dd.pdf} }