@article { author = {Asadi, Marjan and Gholampour, Mohammad Ali and Kompani, Farzad and Alizadeh, Shaban}, title = {Expression of Long Non-Coding RNA H19 in Acute Lymphoblastic Leukemia}, journal = {Cell Journal (Yakhteh)}, volume = {25}, number = {1}, pages = {1-10}, year = {2023}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2022.8315}, abstract = {Objective:Long non-coding RNA (lncRNA) H19 has essential roles in growth, migration, invasion, and metastasis ofmost cancers. H19 dysregulation is present in a large number of solid tumors and leukemia. However, the expressionlevel of H19 in acute lymphoblastic leukemia (ALL) has not been elucidated yet. The current study aimed to exploreH19 expression in ALL patients and cell lines.Materials and Methods:This experimental study was conducted in bone marrow (BM) samples collected from 25patients with newly diagnosed ALL. In addition, we cultured the RPMI-8402, Jurkat, Ramos, and Daudi cell linesand assessed the effects of internal (hypoxia) and external (chemotherapy medications L-asparaginase [ASP] andvincristine [VCR]) factors on h19 expression. The expressions of H19, P53, c-Myc, HIF-1α and β-actin were performedusing quantitative real-time polymerase chain reaction (qRT-PCR) method.Results:There was significantly increased H19 expression in the B-cell ALL (B-ALL, P<0.05), T-cell ALL (T-ALL,P<0.01) patients and the cell lines. This upregulation was governed by the P53, HIF-1α, and c-Myc transcriptionfactors. We observed that increased c-Myc expression induced H19 expression; however, P53 adversely affected H19expression. In addition, the results indicated that chemotherapy changed the gene expression pattern. There was aconsiderable decrease in H19 expression after exposure to chemotherapy medications; nonetheless, hypoxia inducedH19 expression through P53 downregulation.Conclusion:Our findings suggest that H19 may have an important role in pathogenesis in ALL and may act as apromising and potential therapeutic target.}, keywords = {Acute lymphoblastic leukemia,lncRNA,H19,Hypoxia}, url = {https://www.celljournal.org/article_253362.html}, eprint = {https://www.celljournal.org/article_253362_1f50619259aef43b9ec6570910081804.pdf} }