@article { author = {Mardani, Mohammad and Ganji, Rasoul and Ghasemi, Nazem and Kazemi, Mohammad and Razavi, Shahnaz}, title = {Impact of Intraventricular Human Adipose-Derived Stem Cells Transplantation with Pregnenolone Treatment on Remyelination of Corpus Callosum in A Rat Model of Multiple Sclerosis}, journal = {Cell Journal (Yakhteh)}, volume = {24}, number = {12}, pages = {748-756}, year = {2022}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {}, abstract = {ObjectiveMultiple sclerosis (MS) is known as a nerve tissue disorder, which causes demyelination of central nervoussystem (CNS) fibers. Cell-based treatment is a novel strategy for the treatment of demyelinating diseases such asMS. Adipose-derived stem cells (ADSCs) have neuroprotective and neuroregenerative effects and pregnenolone as aneurosteroid has remarkable roles in neurogenesis. We intend to examine the impact of intraventricular transplantationof human ADSCs and systemic injection of pregnenolone on the remyelination of a rat model cuprizone-induceddemyelination.Materials and MethodsThis experimental study was performed on 36 male Wistar rats that received a regular dietand a cuprizone diet for 3 weeks for M.S. induction. Through lipoaspirate surgery, human-ADSCs (hADSCs) wereobtained from a patient. Six groups of rats (n=6): healthy, MS, sham, pregnenolone injection, ADSCs transplantation,and pregnenolone injection/ADSCs transplantation were included in this study. For assessment of remyelination,transmission electron microscopy (TEM), immunohistochemistry staining, real-time reverse transcription-polymerasechain reaction (RT-PCR), and enzyme-linked immunosorbent assay (ELISA) were performed.ResultsTEM outcomes revealed an increase in the thickness of the fibers myelin in the treatment groups (P<0.05). Wealso observed a significant upregulation of MBP, PDGFR-α, and MOG after treatment with hADSCs and pregnenolonecompared to other study groups (P<0.001). These results were confirmed by immunostaining analysis. Moreover, therewas no significant difference between the ADSCs/pregnenolone group and the control group regarding the level ofMBP, A2B5, and MOG proteins in ELISA.ConclusionOur data implied that the remyelination and cell recovery were more improved by intraventricular ADSCstransplantation and pregnenolone injection after inducing a rat model of MS.}, keywords = {Adipose-Derived Stem Cells,intraventricular,multiple sclerosis,Pregnenolone}, url = {https://www.celljournal.org/article_253345.html}, eprint = {https://www.celljournal.org/article_253345_b7a3f9fd03854243926dd8b750854c29.pdf} }