@article { author = {Vosough, Massoud and Nikfam, Sepideh and Torabi, Shukoofeh and Sadri, Bahareh and Ahmadi Amoli, Hadi and Basi, Ali and Niknejadi, Maryam and Hossein-Khannazer, Nikoo and Hosseini, Seyedeh-Esmat and Mardpour, Soura and Azimian, Vajiheh and Jaroughi, Neda and Aghdami, Nasser and Amirzehni, Hamid Reza and Anushirvani, Amir and Malekzadeh, Reza and Baharvand, Hossein and Mohamadnejad, Mehdi}, title = {Mesenchymal Stromal Cell Therapy Improves Refractory Perianal Fistula In Crohn’s Disease: Case Series Clinical Interventional Study}, journal = {Cell Journal (Yakhteh)}, volume = {24}, number = {2}, pages = {62-68}, year = {2022}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2022.7981}, abstract = {Objective: Perianal fistulas in Crohn’s disease (CD) are the main challenges in inflammatory bowel diseases (IBDs). Some of the fistulas are refractory to any therapeutic strategy. The aim of this study was to evaluate the therapeutic effects of mesenchymal stromal cells (MSCs) as a novel promising modality for the treatment of fistulizing CD.Materials and Methods: This case series clinical interventional study was conducted from 2014 to 2017 at Shariati Hospital, an IBD referral center in Tehran, Iran. Refractory adult patients with CD who had draining perianal fistulas were enrolled in this study. All patients were examined by a colorectal surgeon and the fistula imaging studies were performed by pelvic magnetic resonance imaging (MRI). After autologous bone marrow (BM) aspiration and MSCs isolation, the cells were cultured and passaged under current good manufacturing practice (cGMP) conditions. Four intra-fistula injections of cells, each containing 40×106 MSCs suspended in fibrin glue, were administered by an expert surgeon every 4 weeks. Procedure safety, feasibility and closure of the perianal fistulas at week 24 were assessed. Clinical examination and MRI findings were considered as the primary end points.Results: In total, 5 patients (2 males and 3 females) were enrolled in this study. No adverse events were observed during the six-month follow-up in these patients. Both the Crohn’s Disease Activity Index (CDAI) and Perianal Disease Activity Index (PDAI) scores decreased in all patients after cell injections and one patient achieved complete remission with closure of fistulas, discontinuation of fistula discharge, and closure of the external opening.Conclusion: Local injection of MSCs combined with fibrin glue is potentially a safe and effective therapeutic approach for complex perianal fistulas in patients with CD.}, keywords = {Cell therapy,Crohn’s Disease,Mesenchymal Stromal Cells,Perianal Fistulas}, url = {https://www.celljournal.org/article_251731.html}, eprint = {https://www.celljournal.org/article_251731_24be43b85c26f94202817989304f5334.pdf} }