@article { author = {Jeong, Da Som and Kim, Yu Cheon and Hoon Oh, Ji and Hee Kim, Myoung}, title = {Akt1 Decreases Gcn5 Protein Stability Through Regulating The Ubiquitin-Proteasome Pathway In Mouse Embryonic Fibroblasts}, journal = {Cell Journal (Yakhteh)}, volume = {24}, number = {1}, pages = {51-54}, year = {2022}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2022.7961}, abstract = {General control non-derepressible 5 (Gcn5) is a member of histone acetyltransferase (HAT) that plays key roles duringembryogenesis as well as in the development of various human cancers. Gcn5, an epigenetic regulator of Hoxc11, has been reported to be negatively regulated by Akt1 in the mouse embryonic fibroblasts (MEFs). However, the exact mechanism by which Akt1 regulates Gcn5 is not well understood. Using protein stability chase assay, we observed thatGcn5 is negatively regulated by Akt1 at the post-translational level in MEFs. The stability of Gcn5 protein is determined by the competitive binding with the protein partner that interacts with Gcn5. The interaction of Gcn5 and Cul4a-Ddb1complex predominates and promotes ubiquitination of Gcn5 in the wild-type MEFs. On the other hand, in the Akt1-null MEFs, the interaction of Gcn5 and And-1 inhibits binding of Gcn5 and Cul4a-Dbd1 E3 ubiquitin ligase complex, therebyincreasing the stability of the Gcn5 protein. Taken together, our study indicates that Akt1 negatively controls Gcn5 via the proteasomal degradation pathway, suggesting a potential mechanism that regulates the expression of Hox genes.}, keywords = {Akt1,Gcn5,Proteasome Endopeptidase Complex,Ubiquitin}, url = {https://www.celljournal.org/article_251729.html}, eprint = {https://www.celljournal.org/article_251729_afb901f6a3100d5b305d04555e12adba.pdf} }