@article { author = {Ahmadalizadeh Khanehsar, Mona and Hoseinbeyki, Moslem and Fakhr Taha, Masoumeh and Javeri, Arash}, title = {Repression of TGF-β Signaling in Breast Cancer Cells by miR-302/367 Cluster}, journal = {Cell Journal (Yakhteh)}, volume = {21}, number = {4}, pages = {444-450}, year = {2019}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2020.6193}, abstract = {ObjectiveEpigenetic alterations of the malignantly transformed cells have increasingly been regarded as an important event in the carcinogenic development. Induction of some miRNAs such as miR-302/367 cluster has been shown to induce reprogramming of breast cancer cells and exert a tumor suppressive role by induction of mesenchymal to epithelial transition, apoptosis and a lower proliferation rate. Here, we aimed to investigate the impact of miR-302/367 overexpression on transforming growth factor-beta (TGF-β) signaling and how this may contribute to tumor suppressive effects of miR-302/367 cluster. Materials and Methods In this experimental study, MDA-MB-231 and SK-BR-3 breast cancer cells were cultured and transfected with miR-302/367 expressing lentivector. The impact of miR-302/367 overexpression on several mediators of TGF-β signaling and cell cycle was assessed by quantitative real-time polymerase chain reaction (qPCR) and flow cytometry. Results Ectopic expression of miR-302/367 cluster downregulated expression of some downstream elements of TGF-β pathway in MDA-MB-231 and SK-BR-3 breast cancer cell lines. Overexpression of miR-302/367 cluster inhibited proliferation of the breast cancer cells by suppressing the S-phase of cell cycle which was in accordance with inhibition of TGF-β pathway. Conclusion TGF-β signaling is one of the key pathways in tumor progression and a general suppression of TGF-β mediators by the pleiotropically acting miR-302/367 cluster may be one of the important reasons for its anti-tumor effects in breast cancer cells.}, keywords = {Breast cancer,MiR,302/367,Reprogramming,Transforming Growth Factor,Beta}, url = {https://www.celljournal.org/article_250609.html}, eprint = {https://www.celljournal.org/article_250609_fa12ccd7244d1ff5aef12ef3ab7e0aad.pdf} }