@article { author = {Saylan, Aslihan and Duman, Selcuk}, title = {Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method}, journal = {Cell Journal (Yakhteh)}, volume = {18}, number = {1}, pages = {83-88}, year = {2016}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2016.3990}, abstract = {ObjectiveIn 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. Materials and MethodsThis observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. Human serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups annexin V was used to determine the rate of apoptosis of these sperm. ResultsFollowing swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher apoptosis rate in the HSA group compared to the HA group. ConclusionThe addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility.}, keywords = {Annexin V,Apoptosis,Hyaluronic acid,human}, url = {https://www.celljournal.org/article_250349.html}, eprint = {https://www.celljournal.org/article_250349_3a8bc55687ee9410672a20231ff8fdef.pdf} }