@article { author = {Rajabi Fomeshi, Motahareh and Ebrahimi, Marzieh and Mowla, Seyed Javad and Firouzi, Javad and Khosravani, Pardis}, title = {CD133 Is Not Suitable Marker for Isolating Melanoma Stem Cells from D10 Cell Line}, journal = {Cell Journal (Yakhteh)}, volume = {18}, number = {1}, pages = {21-27}, year = {2016}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2016.3983}, abstract = {ObjectiveCutaneous melanoma is the most hazardous malignancy of skin cancer with a high mortality rate. It has been reported that cancer stem cells (CSCs) are responsible for malignancy in most of cancers including melanoma. The aim of this study is to compare two common methods for melanoma stem cell enriching; isolating based on the CD133 cell surface marker and spheroid cell culture. Materials and MethodsIn this experimental study, melanoma stem cells were enriched by fluorescence activated cell sorting (FACS) based on the CD133 protein expression and spheroid culture of D10 melanoma cell line,. To determine stemness features, the mRNA expression analysis of ABCG2, c-MYC, NESTIN, OCT4-A and -B genes as well as colony and spheroid formation assays were utilized in unsorted CD133+, CD133- and spheroid cells. Significant differences of the two experimental groups were compared using student’s t tests and a two-tailed value of P < 0.05 was statistically considered as a significant threshold. ResultsOur results demonstrated that spheroid cells had more colony and spheroid forming ability, rather than CD133+ cells and the other groups. Moreover, melanospheres expressed higher mRNA expression level of ABCG2, c-MYC, NESTIN and OCT4-A com- pared to other groups (P < 0.05). ConclusionAlthough CD133+ derived melanoma cells represented stemness fea- tures, our findings demonstrated that spheroid culture could be more effective meth- od to enrich melanoma stem cells.}, keywords = {Melanoma,Cancer Stem Cell,CD133,Spheroid}, url = {https://www.celljournal.org/article_250342.html}, eprint = {https://www.celljournal.org/article_250342_25ef0d4ca714e2b96701d53d5b0f74aa.pdf} }