@article { author = {Khorsandi, Layasadat and Saremy, Sadegh and Khodadadi, Ali and Dehbashi, Fereshteh}, title = {Effects of Exendine-4 on The Differentiation of Insulin Producing Cells from Rat Adipose-Derived Mesenchymal Stem Cells}, journal = {Cell Journal (Yakhteh)}, volume = {17}, number = {4}, pages = {720-729}, year = {2016}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {10.22074/cellj.2016.3844}, abstract = {ObjectiveTo evaluate the effect of Exendine-4 (EX-4), a Glucagon-like peptide 1 (GLP-1) receptor agonist, on the differentiation of insulin-secreting cells (IPCs) from rat adipose-derived mesenchymal stem cells(ADMSCs). Materials and MethodsIn this experimental study, ADMSCs were isolated from rat adi- pose tissue and exposed to induction media with or without EX-4. After induction, the existence of IPCs was confirmed by morphology analysis, expression pattern analysis of islet-specific genes (Pdx-1, Glut-2 and Insulin) and insulin synthesis and secretion. ResultsIPCs induced in presence of EX-4 were morphologically similar to pancre- atic islet-like cells. Expression of Pdx-1, Glut-2 and Insulin genes in EX-4 treated cells was significantly higher than the cells exposed to differentiation media without EX-4. Compared to EX-4 untreated ADMSCs, insulin release from EX-4 treated ADMSCs showed a nearly 2.5 fold (P < 0.05) increase when exposed to a high glucose (25 mM) medium. The percentage of insulin positive cells in the EX-4 treated group was ap- proximately 4-fold higher than in the EX-4 untreated ADMSCs. ConclusionThe present study has demonstrated that EX-4 enhances the differen- tiation of ADMSCs into IPCs. Improvement of this method may help the formation of an unlimited source of cells for transplantation.}, keywords = {Exendin,4,Differentiation,Insulin,Secreting Cells,Adipose tissue,Mes,enchymal Stem Cells}, url = {https://www.celljournal.org/article_250335.html}, eprint = {https://www.celljournal.org/article_250335_2c13779ab961b319b7f10bdd04f1c844.pdf} }