@article { author = {Hajizadeh-Sikaroodi, Shohreh and Hosseini, Ahmad and Fallah, Ali and Estiri, Hajar and Noormohammadi, Zahra and Salehi, Mohammad and Ghaderian, Sayyed Mohammad Hossein and Akhavan Niaki, Haleh and Soleimani, Masoud and Kazemi, Bahram}, title = {Lentiviral Mediating Genetic Engineered Mesenchymal Stem Cells for Releasing IL-27 as a Gene Therapy Approach for Autoimmune Diseases}, journal = {Cell Journal (Yakhteh)}, volume = {16}, number = {3}, pages = {255-262}, year = {2014}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {}, abstract = {ObjectiveAutoimmune diseases precede a complex dysregulation of the immune system. T helper17 (Th17) and interleukin (IL)-17 have central roles in initiation of inflammation and subsequent autoimmune diseases. IL-27 significantly controls autoimmune diseases by Th17 and IL-17 suppression. In the present study we have created genetic engineered mesenchymal stem cells (MSCs) that mediate with lentiviral vectors to release IL-27 as an adequate vehicle for ex vivo gene therapy in the reduction of inflammation and autoimmune diseases. Materials and Methods In this experimental study, we isolated adipose-derived MSCs (AD-MSCs) from lipoaspirate and subsequently characterized them by differentiation. Two subunits of IL-27 (p28 and EBI3) were cloned in a pCDH-513B-1 lentiviral vector. Expressions of p28 and EBI3 (Epstein-Barr virus induced gene 3) were determined by real time polymerase chain reaction (PCR). MSCs were transduced by a pCDH-CMV-p28-IRES- EBI3-EF-copGFP-Pur lentiviral vector and the bioassay of IL-27 was evaluated by IL-10 expression. Results Cell differentiation confirmed true isolation of MSCs from lipoaspirate. Restriction enzyme digestion and sequencing verified successful cloning of both p28 and EBI3 in the pCDH-513B-1 lentiviral vector. Real time PCR showed high expressions level of IL-27 and IL-10 as well as accurate activity of IL-27. Conclusion The results showed transduction of functional IL-27 to AD-MSCs by means of a lentiviral vector. The lentiviral vector did not impact MSC characteristics.}, keywords = {Autoimmune Disease,Gene therapy,IL,27,Mesenchymal stem cells}, url = {https://www.celljournal.org/article_250219.html}, eprint = {https://www.celljournal.org/article_250219_93419ad64c0c929b88d74a513c6d2405.pdf} }