@article { author = {Ataee, R.A. and Ali Zadegan, M. Gorban and Hajia, M. and Tavana, A. Mehrabi and Godarzi, Z. and Nakhjavani, F.}, title = {Rapid Diagnosis Of Neisseria Meningitides By PCR Method}, journal = {Cell Journal (Yakhteh)}, volume = {8}, number = {2}, pages = {92-97}, year = {2006}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {}, abstract = {Introduction: The aim of this study was to setup, optimize and introduce a sensitive and specific PCR detection method for identification of Neisseria meningitidis DNA in clinical samples.Material and Methods: Capsular transport gene A (ctrA) was selected as a specific target sequence. This primer pair amplifies 101bp of the target gene. Neisseria meningitidis strain: ATCC; 13090 and Neisseria meningitides serogroup C were used as a standard organism for optimization experiments. A range of bacterial pathogens were used for specificity testing, including, Haemophilus influenzae Type b: ATCC; 49766, Escherichia coli: ATCC;35218, Enterobacter, Klebsiella pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus group D. Phenol – chloroform method was used for DNA extraction. Amplified product was detected by gel agarose electrophoresis, stained by ethidiome bromide.Results: Our results confirmed amplification of the expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg of Neisseria meningitidis DNA as a final detection limit.Conclusion: As a conclusion, PCR is a method with high sensivity and specificity and specificity which can be performed within 3 hours, and therefore utilization of this test in the clinical laboratories can help rapid diagnosis of Neisseria meningitides in clinical samples.}, keywords = {Meningitis,NEISSERIA MENINGITIDIS,PCR,ctrA Gene}, url = {https://www.celljournal.org/article_248426.html}, eprint = {https://www.celljournal.org/article_248426_db5997174a56bb917fc12f94c429a614.pdf} }