@article { author = {Mohammadi Motlagh, Hamid Reza and Mansouri, Kamran and Shakiba, Yadollah and Keshavarz, Maryam and Khodarahmi, Reza and Siami, Abbas and Mostafaie, Ali}, title = {Anti-Angiogenic Effect Of Aqueous Extract Of Shallot (Allium Ascalonicum) Bulbs In Rat Aorta Ring Model}, journal = {Cell Journal (Yakhteh)}, volume = {11}, number = {2}, pages = {190-195}, year = {2009}, publisher = {Royan Institute, Iranian Academic Center for Education Culture and Research (ACECR)}, issn = {2228-5806}, eissn = {2228-5814}, doi = {}, abstract = {Objective: Angiogenesis is a key process in the promotion of cancer and its metastasis. Many natural health products inhibit angiogenesis. Because of the lack of molecular studies on anti-tumor and anti-angiogenic effects of shallot (Allium ascalonicum); except a few clinical studies on other shallot properties, such as the anti-proliferative effect of shallot chloroformic extract on two tumor cell lines, the present study focuses on the anti-angiogenic effect of aqueous shallot extract using an aorta ring model.Materials and Methods: Aortic rings were obtained by cross-sectioning, at 1-2 mm intervals, the thoracic aorta of 4-8 weeks old Wistar male rats and cultured them in a thin drop of type I collagen gel. After 3 days of culturing and first sprouting, the extract of A. ascalonicum (from 25 to 800 μg/ml) was added to cultures. The results of anti-angiogenic activity were investigated by microscope. The cytotoxicity of extract at different doses on HUVECs was measured by trypan blue assay.Results: The results showed that the shallot extract has suitable anti-angiogenic effect in a range of 50 to 800 μg/ml, but in 25 μg/ml, the extract has no considerable effect. In addition, a tangible cytotoxic effect on endothelial cells at the above mentioned doses was observed.Conclusion: Our study showed that aqueous extract of A. ascalonicum bulbs has noticeable anti-angiogenic activity without toxic effect on the cells in doses that ranged from 50-800 μg/ ml. Therefore, A. ascalonicum can be a potential candidate for further investigations used in angiogenesis-related pathologic conditions.}, keywords = {Angiogenesis,Allium ascalonicum,Aortic Rings,Endothelial cells,Aqueous extract}, url = {https://www.celljournal.org/article_248127.html}, eprint = {https://www.celljournal.org/article_248127_48a096cb4ebec8e64cff650f142deb97.pdf} }